Reactions of Some Hordeum Genotypes to Rhynchosporium commune and Investigation of Rrs1 Resistance Locus in Resistant Genotypes Using Molecular Markers

Öz

The fungus Rynchosporium commune causes scald in barley. In this study, 27 barley cultivars, 15 barley landraces, 10 Hordeum spontaneum and 8 Hordeum bulbosum genotypes were evaluated to scald and 5 cultivars (Avcı 2002, Çetin 2000, Sinanbey, Cirit, Cacabey), 3 Hordeum spontaneum genotypes (Hs4, Hs8, Hs9) and 4 Hordeum bulbosum genotypes (Hb2, Hb3, Hb4, Hb8) were found to be resistant. None of the fifteen barley landraces showed resistance to the disease. The presence of Rrs1 resistance gene in barley genotypes against R. commune was investigated by testing the resistant barley genotypes with CAPS markers (11_1476, 11_0010, 11_0823 and 11_0315) indicating the region closest to the Rrs1 locus and markers associated with barley leaf scald disease resistance genes (HVS3-SCAR, Bmac209-SSR, Bmac67-SSR, Ebmac871-SSR, Bmag0006-SSR). In the 11_0823 CAPS marker closest to the region, a resistant band profile was observed in Avcı 2002, Sinanbey, Cirit, and Cacabey varieties. In the HVS3-SCAR marker, Avcı 2002, Sinanbey, Cirit, Cacabey varieties and Hb2, Hb3, Hb4 H. bulbosum genotypes; in the Ebmac871-SSR and Bmac209-SSR markers, Avcı 2002 and Sinanbey varieties and Hs4, Hs8 and Hs9 H. spontaneum genotypes; in the Bmag 0006-SSR marker, Çetin 2000, Cirit and Cacabey varieties and Hs4, Hs8 and Hs9 H. spontaneum genotypes; in the Bmac67-SSR marker, Çetin 2000, Cirit and Cacabey varieties and Hs8 H. spontaneum genotype resistant band profiles were observed. The best and most sustainable way to control barley leaf scald disease caused by Rhynchosporium commune is to utilize resistant varieties. Introgressing and pyramiding different loci through marker-assisted selection is crucial for resistance breeding. These markers used for tagging Rrs1 barley leaf scald resistance may facilitate pyramiding studies and marker-assisted selection in breeding programs.

Anahtar Kelimeler

• Rhynchosporium commune
• Scald
• Rrs1
• Barley
• Barley landraces
• Wild barley

Etik Beyan

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Bazı Hordeum Genotiplerinin Rhynchosporium commune’ye Karşı Reaksiyonları ve Dayanıklı Olarak Belirlenen Genotiplerde Rrs1 Dayanıklılık Lokusunun Moleküler Markörler Kullanılarak Araştırılması

Öz

Rhynchosporium commune fungusu konukçusu olduğu arpa bitkisinde arpa yaprak lekesi hastalığına neden olmaktadır. Bu çalışmada, 27 arpa çeşidinin, 15 yerel arpa çeşidinin, 10 Hordeum spontaneum, 8 Hordeum bulbosum yabani arpa genotipinin arpa yaprak lekesi hastalığına karşı reaksiyonları değerlendirilmiş ve 5 çeşit (Avcı 2002, Çetin 2000, Sinanbey, Cirit, Cacabey), 3 adet H. spontaneum genotipi (Hs4, Hs8, Hs9) ve 4 adet Hordeum bulbosum genotipi (Hb2, Hb3, Hb4, Hb8) dayanıklı olarak belirlenmiştir. On beş arpa yerel çeşidinin hiçbiri hastalığa karşı dayanıklılık göstermemiştir. Dayanıklı olarak belirlenen arpa genotipleri Rrs1 lokusuna en yakın bölgeyi işaret eden, CAPS markörleri (11_1476, 11_0010, 11_0823 ve 11_0315) ve arpa yaprak lekesi hastalığı dayanıklılık genleri ile ilişkili markörler (HVS3-SCAR, Bmac209-SSR, Bmac67-SSR, Ebmac871-SSR, Bmag0006-SSR) ile test edilerek R. commune etmenine karşı arpa genotiplerinde Rrs1 dayanıklılık geninin varlığı araştırılmıştır. Bölgeye en yakın olan 11_0823 CAPS marköründe Avcı 2002, Sinanbey, Cirit ve Cacabey çeşitlerinde dayanıklı bant profili belirlenmiştir. HVS3-SCAR marköründe Avcı 2002, Sinanbey, Cirit, Cacabey çeşitlerinde ve Hb2, Hb3, Hb4 H. bulbosum genotiplerinde, Ebmac871-SSR ve Bmac209-SSR markörlerinde Avcı 2002 ve Sinanbey çeşitleri ile Hs4, Hs8 ve Hs9 H. spontaneum genotipleri, Bmag 0006-SSR marköründe Çetin 2000, Cirit ve Cacabey çeşitleri ile Hs4, Hs8 ve Hs9 H. spontaneum genotiplerinde, Bmac67-SSR marköründe ise Çetin 2000, Cirit ve Cacabey çeşitleri ile Hs8 H. spontaneum genotiplerinde dayanıklı bant profili saptanmıştır. Rhynchosporium commune etmeninin neden olduğu arpa yaprak lekesi hastalığı ile mücadelede etkili ve sürdürülebilir kontrol yöntemi dayanıklı çeşit kullanımıdır. Markör destekli seleksiyon kullanılarak farklı lokusların introgresyonu ve piramitlenmesi dayanıklılık ıslahı açısından önemlidir. Rrs1 arpa yaprak lekesi hastalığına dayanıklılığın etiketlenmesi için kullanılan bu markörlerin ıslah programlarında piramitleme ve markör destekli seçimi kolaylaştıracağı düşünülmektedir.

Anahtar Kelimeler

• Rhynchosporium commune
• Arpa yaprak lekesi hastalığı
• Rrs1
• Arpa
• Arpa yerel çeşitleri
• Yabani arpa

Etik Beyan

Bu çalışma için etik kuruldan izin alınmasına gerek yoktur.

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