Comparative Analysis of the Physico-chemical and Microbiological Profiles of Mammalian Milk: A Multi-Species Study on Cow, Sheep, Goat, Camel, Buffalo, Iraqi Reem Gazelle (Gazella subgutturosa marica), and Human Milk

Öz

The current study presents a comprehensive assessment of the physico-chemical properties, mineral and trace element concentrations, and microbiological analysis of milk samples collected from different type of animals including cow, sheep, goat, buffalo, camel, Iraqi Reem Gazelle (Gazella subgutturosa marica) and Mother milk, from different regions in Salah Al-Din, Kirkuk, and Sulaymaniyah Governorates. ANOVA analysis revealed a significant difference (P≤0.01) among different types of analyzed milk samples in all determined characterizations. Significantly, gazelle milk was statistically increased in the percentages of fat, total proteins, total solids, ash, solid non-fat and calorific values were respectively reached (10.32%, 7.40%, 18.61%, 1.163%, 13.24%, 137.58 Kcal/100 g). Besides that, mother milk was a significant increase in lactose and water contents were (6.81% and 88.22%), whereas goat milk showed high value in water content (87.38%). In physical properties, buffalo and cow milk were significantly (P≤0.01) higher than other species in freezing point which indicated as (- 0.518 and - 0.534 °C). Iraqi Reem Gazelle (Gazella subgutturosa marica) milk distinguished increased of specific gravity and titratable acidity were reached (1.039 and 0.271%), while mother milk was superior in pH value (7.46). Regarding to mineral and trace element levels, Iraqi Reem Gazelle (Gazella subgutturosa marica) milk has better in Ca, P, and Fe levels were (2691.93, 1922.37, 12.30 PPM), when goat milk showed a more favorable Mg and K concentrations were reached (1223.93 and 1815.20 PPM), meanwhile, camel milk was notable for its high concentrations of Cl and Na were reached (2841.93 and 591.40 PPM). According to microbial analysis which indicated that superior of goat milk in total count, Coliform count and yeast were (7.11, 2.94 and 2.79 CFU/mL), while camel milk recorded higher averages in the coliform count was (3.16 CFU/mL), then cow milk was superior in molds count (1.65 CFU/mL). Also, human and gazelle milk was superior in methylene blue reduction test. The results demonstrated that found many significantly differences within all analyzed milk samples in their chemical, physical, mineral levels and microbiological analysis, which this highlights the qualitative differences in milk quality and is considered an important indicator in assessing the safety and quality of different dairy products.

Anahtar Kelimeler

• Milk species
• Physical
• Chemical properties
• Microbiological
• Mineral analysis

Proje Numarası

Not applicable

Etik Beyan

The present study was conducted following the international and national ethical standards. Ethical approval was obtained from the Research Ethics Committees of Tikrit University and Community of ethical board in Kirkuk Health Directorate (Approval No: 552 in 01October 2024) – Republic of Iraq.

Teşekkür

The authors would like to thanks College of Agriculture – University of Kirkuk for providing research lab facilities and many gratitude to Kirkuk Health Directories and Al Naser Hospital (Woman and children Hospital) for their kind help with Human milk. Great thanks to Faculties of Food science and Basic Education in Shirqat, Tikrit University for all their facilities and assistance helps.

Kaynakça

1. Abdulwahid, J. and Al-Fayad, M. (2022). Evaluation of different chemical and physical components of milk in cows, buffaloes, sheep, and goats. Archives of Razi Institute, 77(1): 477–481.
2. Abuelnaga, A. Ata, N., Abd El, K., Hedia, R., Soliman, M., Kandil, M. and Arafa, A. (2022). Microbiological evaluation and molecular discrimination of milk samples from humans and different animals. World's Veterinary Journal, 12(1): 9-18.
3. Ahmida, N., Shaboun, S. and Ahmida, M. (2021). Comparative study on the physiochemical and nutritional properties of fresh milk samples collected from farms animals in Benghazi City, Libya. Journal of Pure & Applied Sciences, 20(2): 49-53.
4. Alatalo, D. and Hassanipour, F. (2020). An experimental study on human milk rheology: Behavior changes from external factors. Fluids, 5(2): 42-61.
5. Aldhalemi, M. and Al-Mosawi, R. (2019). Study the effect of lactation period on the Biochemical properties of breast milk during the first year of Breast-feeding. Kufa Journal for Agricultural Sciences, 11(2):‏ 11-18. Alebie, A., Molla, A., Adugna, W., Tesfaye, A. and Ejo, M. (2021). Prevalence, isolation, identification, and risk factors of major bacterial cause of camel subclinical mastitis. Bio Med Research International, 2021(1): 1-6.
6. Alhassani W. (2024). Camel milk: Nutritional composition, therapeutic properties, and benefits for human health. Open Veterinary Journal, 14(12): 3164–3180.
7. Allen, L., Islam, M., Kac, G., Michaelsen, K., Moore, S., Andersson, M. and Figueiredo, A. (2025). Reference values for minerals in human milk: The mothers, infants and lactation quality (MILQ) study. Advances in Nutrition, 16(1): 1-15. ‏
8. Amr, M. and Farid, A. (2024). Impact of cow, buffalo, goat or camel milk consumption on oxidative stress, inflammation and immune response post weaning time. Scientific Reports, 14(1): 9967- 9978.

9. Anum, N., Lashari, M., Riaz, R., Ali, A., Ahmed, H. and Tahir, M. (2024). Evaluation of proximate composition and mineral profile of raw milk from three livestock species. Insights in Animal Science, 1(2): 31-36. ‏
10. AOAC (2019) Official Methods of Analysis of the Association of Official Analytical Chemists: Official Methods of Analysis of AOAC International. 21st Edition, AOAC, Washington DC, U. S. A.
11. AOAC (2023) Official Methods of Analysis of the Association of Official Analytical Chemists: Official Methods of Analysis of AOAC International. 22st Edition, AOAC, Washington DC, U. S. A.
12. Aqeela, S., Thania, I. and Ibrahimb, M. (2021). Treated cow milk quality analysis in high-temperature short time (HTST) thermal treatment using f-value and methylene blue reduction test (MBRT). Journal of Agro Biotechnology, 12(1S): 124-132.
13. Arrichiello, A., Auriemma, G. and Sarubbi, F. (2022). Comparison of nutritional value of different ruminant milks in human nutrition. International Journal of Functional Nutrition, 3(4): 1-10.
14. Bala, F. (2024). Human milk nutrients: the influence of maternal diet and nutritional status. International Journal Health Science Research, 14(4): 394-405. ‏
15. Barłowska, J., Szwajkowska, M., Litwińczuk, Z. and Król, J. (2011). Nutritional value and technological suitability of milk from various animal species used for dairy production. Comprehensive Reviews in Food Science and Food Safety, 10(6): 291-302.
16. Βasdeki, A. M., Fatouros, D. G., Βiliaderis, C. G. and Moschakis, T. (2021). Physicochemical properties of human breast milk during the second year of lactation. Current Research in Food Science, 8(4): 565-576. ‏ Berruga, M., de la Vara, J., Licón, C., Garzón, A., García, A. J., Carmona, M. and Molina, A. (2021). Physicochemical, microbiological and technological properties of red deer (Cervus elaphus) milk during lactation. Animals, 11(3): 906-920.
17. Bindulekha, D. (2019). Determination of microbiological quality of milk from different breeds of cattle using methylene blue reduction test. Journal of Emerging Technologies and Innovative Research, 6(5): 613-618.
18. Björklund, K., Vahter, M., Palm, B., Grandér, M., Lignell, S. and Berglund, M. (2012). Metals and trace element concentrations in breast milk of first time healthy mothers: a biological monitoring study. Environmental Health, 11(1): 92-100.
19. Boquien, C. (2018). Human Milk: An ideal food for nutrition of preterm newborn. Frontier in Pediatrics. 16(6): 295- 304.
20. Brockway, M., Daniel, A., Reyes, S., Gauglitz, J., Granger, M., Mc Dermid, J. and Azad, M. (2024b). Human milk bioactive components, child growth, and body composition in the first 2 years: a systematic review. Advances in Nutrition, 15(1): 100127 - 100140. ‏
21. Brockway, M., Daniel, A., Reyes, S., Granger, M., McDermid, J., Chan, D., Refvik, R., Sidhu, K., Musse, S., Patel, P., Monnin, C., Lotoski, L., Geddes, D., Jehan, F., Kolsteren, P., Allen, L., Hampel, D., Eriksen, K. G., Rodriguez, N. and Azad, M. (2024a). Human milk macronutrients and child growth and body composition in the first two years: A systematic review. Advances in Nutrition, 15(1): 100149 - 100178.
22. Butts, C., Hedderley, D., Herath, T., Paturi, G., Glyn-Jones, S., Wiens, F., Stahl, B. and Gopal, P. (2018). Human milk composition and dietary intakes of breastfeeding women of different ethnicity from the Manawatu-Wanganui Region of New Zealand. Nutrients, 10(9): 1231- 1247.
23. Ceniti, C., Britti, D., Trimboli, F., Morittu, V., Lopreiato, V. and Costanzo, N. (2019). Evaluation of freezing point in milk from buffaloes reared in Calabria, Italy. Italian Journal of Food Safety, 8(2): 7895- 7899.
24. Chen, Z., Leinisch, F., Greco, I., Zhang, W., Shu, N., Chuang, C. and Davies, M. (2019). Characterisation and quantification of protein oxidative modifications and amino acid racemisation in powdered infant milk formula. Free Radical Research, 53(1): 68–81.
25. Chenouf, A., Yabrir, B., Khirani, A., Boukerch, A., Hakem, A. and Houali, K. (2024). Mineral composition, nitrogen distribution of goat’s milk produced under algerian steppe condition across lactation stage. Rural Sustainability Research, Scientific Journal of Latvia University of Life Sciences and Technologies, 51(346): 20-28.
26. Choudhary, V., Bais, B., Kumar, Y., Goklaney, D., Saran, D., Kanwar, P. and Kumar, A. (2024). Comparative analysis of physico- chemical properties of buffalo, camel and blended (buffalo 70%: Camel 30%) Milk. Journal of Camel Practice and Research, 1(31): 77-81.
27. Cissé, H., Muandze-Nzambe, J., Somda, N., Sawadogo, A., Drabo, S., Tapsoba, F., Zongo, C., Traoré, Y. and Savadogo, A. (2019). Assessment of safety and quality of fermented milk of camels, cows, and goats sold and consumed in five localities of Burkina Faso. Veterinary World, 12(2): 295–304.
28. Claeys W., Verraes C., Cardoen S., De Block J., Huyghebaert A., Raes K., Dewettinck K. and Herman L. (2014). Consumption of raw or heated milk from different species: An evaluation of the nutritional and potential health benefits, Food Control, 45(3): 188-201.
29. de la Vara, J., Berruga, M., Cappelli, J., Landete-Castillejos, T., Carmona, M., Gallego, L. and Molina, A. (2018). Some aspects of the ethanol stability of red deer milk (Cervus elaphus hispanicus): A comparison with other dairy species. International Dairy Journal, 139 (86): 103-109.
30. Demmelmair, H. and Koletzko, B. (2018). Lipids in human milk. Best practice & research. Clinical Endocrinology and Metabolism, 32(1): 57– 68.
31. Desidera, F., Skeie, S. B., Devold, T. G., Inglingstad, R. A., Smistad, M. and Porcellato, D. (2025). A comparative analysis of goat milk quality on Norwegian farms with focus on somatic cell count and seasonal variation. Journal of Dairy Science, 108(10): 10548-10560.
32. Dewey, K., Wessells, K., Arnold, C., Prado, E., Abbeddou, S., Adu-Afarwuah, S. and Stewart, C. (2021). Characteristics that modify the effect of small-quantity lipid-based nutrient supplementation on child growth: an individual participant data meta-analysis of randomized controlled trials. The American Journal of Clinical Nutrition, 114(1): 15S-42S.
33. Eldeib, S., Damodaran, J., Gad, S., Haddad, H. and Elmesserey, M. (2025). Goat milk vs. cow milk vs human milk? A comprehensive comparison of nutritional composition in infant nutrition. International Journal of Clinical Studies & Medical Case Reports, 48(5): 1-4. Elmahal, S., Elfaki, A. and Ahmed, M. (2018). Study the physical properties of different types of raw milk in Alguzera farms (Sudan).‏ Journal of Applied Physics, 8(4): 44-48.
34. Emakpor, O., Edo, G., Jikah, A., Ikpekoro, V., Agbo, J., Ainyanbhor, I. and Akpoghelie, P. (2024). Buffalo milk: an essential natural adjuvant. Discover Food, 4(1): 38 - 49. Engindeniz, S., Taşkın, T., Gbadamonsi, A., Ahmed, A., Cisse, A., Seioudy, A., Kandemir, Ç. and Koşum, N. (2021). Analysis of preferences for milk and milk products of consumers. Journal of Tekirdağ Agricultural Faculty, 18(3): 470-481. (In Turkish)
35. Esquerra-Zwiers, A., Heter, M., Perecki, A., Jackson, O., Jongekryg, D. and Yurk, B. (2024). Comparison of human milk bottle with infant/toddler test weights in the community setting. BMC nutrition, 10(1): 105-114. ‏
36. Faraz, A., Waheed, A., Tauqir, N., Mirza, R., Ishaq, H. and Nabeel, M. (2020). Characteristics and composition of camel (Camelus dromedarius) milk: The white gold of desert. Advanced Animal Veterinary Science, 8(7): 766-770.
37. Faye, B. and Konuspayeva, G. (2024). Camel milk composition and future market potential. CABI Reviews, 19(1): 1-23.
38. Gagara, M., Sessou, P., Dossa, F., Azokpota, P., Youssao, I., Gouro, S. and Farougou, S. (2022). Hygienic quality of raw and fermented cow milk in the local milk sector of the Liptako-Gourma area in Niger. Veterinary world, 15(6): 1541–1549.
39. Gemechu, T., Eshetu, M., Mummed, Y. Y., Galmessa, U. and Kehaliew, A. (2025). Impact of Climate Change and Variability on the Production and Composition of Dairy Cow Milk in the Central Part of Ethiopia. Food Chemistry Advances, 7: 1-8.
40. Han, B., Zhang, L. and Zhou, P. (2023). Comparative proteomics of whey proteins: New insights into quantitative differences between bovine, goat and camel species. International Journal of Biological Macromolecules, 227(1): 10–16.
41. Hariyani A., Hazra T, Thesiya A. and Ahuja K. (2024). Composition of milk: A comparative analysis on goat, cow and buffalo milk from Amreli region of Gujarat. Indian Journal Animal Health, 63(1): 41-49.
42. IDF (2009). Milk And Milk Products - Determination of Fat Content - General Guidance on The Use of Butyrometric Methods. IDF 152:2009. International Dairy Federation, Brussels, Belgium.
43. Izzadeen, S., Mustafa, N. and Mustafa, M. (2021). Evaluation of morning and evening milk traits in some dairy animals in Erbil city. Tikrit Journal for Agricultural Sciences, 21(3): 75-81. ‏
44. Jandal, J. M. (1996). Comparative aspects of goat and sheep milk. Small Ruminant Research, 22(2): 177-185. Jilo, K. and Tegegne, D. (2016). Chemical composition and medicinal values of camel milk. International Journal of Research Studies in Biosciences, 4(4): 13-25.
45. Jooybari, B. and Ferdosian, F. (2024). Determination of sodium, chlorine, and bromine concentrations in breast milk using neutron activation: Correlation, regression, and machine learning predictions. Journal of Food Composition and Analysis, 136: 106754. ‏
46. Kaskous, S., Alasaad, A., Nouh, A., Mohamed, U., Sauerwein, H., and Bruckmaier, R. (2012). The concentration of lactoferrin and other milk constituents in the Syrian Shami camel during different lactation seasons. Journal of Agricultural Sciences, 28(2): 273-287. ‏(In Arabic)
47. Khaldi, Z., Nafti, M. and Jilani, M. (2022). Baseline composition and microbial quality assessment of raw milk from small ruminants and Maghrebi camels in the oasis area of Tunisia. Tropical Animal Health and Production, 54(6): 391- 402. ‏
48. Kontodimos, I., Chatzimanoli, E., Kasapidou, E., Basdagianni, Z., Karatzia, M., Amanatidis, M. and Margaritis, N. (2023). Characterization of bioactive compounds and element content in goat milk and cheese products. Biology and Life Sciences Forum. 26(1): 98-105.
49. Konuspayeva, G., Faye, B. and Bengoumi, M. (2022). Mineral status in camel milk: A critical review. Animal Frontiers, 12(4): 52-60.
50. Legesse, A., Adamu, F., Alamirew, K. and Feyera, T. (2017). A comparative study on the physicochemical parameters of milk of camel, cow and goat in Somali Regional State, Ethiopia. Chemical Sciences Journal, 8(4): 1-5.
51. Li, S., Saharawat, A., Ye, A., Dave, A. and Singh, H. (2023). Characteristics of red deer (Cervus elaphus) milk: lactational changes in composition and processing impacts on structural and gelation properties. Foods, 12(7): 1517- 1532.
52. Liao, J., Yang, J., Suo, H. and Song, J. (2025). Buffalo milk: Nutritional composition, bioactive properties, and advances in processing technologies-a comprehensive review. Food Chemistry, X(29): 102647 – 102676.
53. Lima, M., Teixeira-Lemos, E., Oliveira, J., Teixeira-Lemos, L., Monteiro, A. and Costa, J. (2018). Nutritional and Health Profile of Goat Products: Focus on Health Benefits of Goat Milk. Eds: Sándor Kukovics, In-Tech Publishing, United Kingdom.
54. Liu, J., Zhu, Z., Huang, Y., Qin, J., Wang, L., Wei, M. and Song, Y. (2025). Comparative analysis of sheep, goat, and cow milk based on non-targeted metabolomics. International Dairy Journal, 168(2): 1-7. ‏
55. Loshi, I., Nikqi, E., Kljusurić, J. and Knights, V. (2024). Mineral composition of fresh cow's milk and the importance of minerals in the human diet. Journal of Hygienic Engineering & Design, 48(2): 30-34.
56. Mahmood, A. and Usman, S. (2010). A comparative study on the physicochemical parameters of milk samples collected from buffalo, cow, goat and sheep of Gujrat, Pakistan. Pakistan Journal of Nutrition, 9(12):1192-1197.
57. Mahrous, E., Abdelgawad, M., Saad, S. and Abdou, S. (2023). Chemical and Bacteriological Assessment of Raw Milk Collected from Some Dairy Farms in El-Behera Governorate, Egypt. Zagazig Veterinary Journal, 51(4): 310-325. ‏
58. Malacarne, M., Franceschi, P., Formaggioni, P., Pisani, G., Petrera, F., Abeni, F. and Summer, A. (2015). Minerals content and distribution in milk from red deer (Cervus elaphus), fallow deer (Dama dama) and roe deer (Capreolus capreolus). Small Ruminant Research, 130: 208-215. ‏
59. Malos, I., Ghizdareanu, A, Vidu, L., Matei, C. and Pasarin, D. (2025). The role of whey in functional microorganism growth and metabolite generation: A biotechnological perspective. Foods, 14(9): 1488.
60. Mandiá, N., Bermejo-Barrera, P., Herbello, P., López-Suárez, O., Fraga, J. M., Fernández-Pérez, C. and Couce, M. (2021). Human milk concentrations of minerals, essential and toxic trace elements and association with selective medical, social, demographic and environmental factors. Nutrients, 13(6): 1-21. ‏
61. McGregor, R. and Poppitt, S. (2013). Milk protein for improved metabolic health: a review of the evidence. Nutrition and Metabolism, 10(1): 46- 59.
62. Melese, A., Egzabher, G., Birara, M. and Kebede, M. (2023). Physicochemical and nutritional characteristics of milk collected from camels, cows and goats in Korahay Zone, Somali Region, Ethiopia: a comparative study. Journal Agricultural Science Food Research, 14(2): 1-11. ‏
63. Melia, S., Yuherman, Y., Ferawati, F., Jaswandi, J., Purwanto, H. and Purwati, E. (2018). Nutrition quality and microbial content of buffalo, cow, and goat milk from West Sumatera. ‏Indonesian Center for Animal Research and Development. 23(3): 150-157.
64. Meng, F., Uniacke-Lowe, T., Ryan, A. and Kelly, A. (2021). The composition and physico-chemical properties of human milk: A review. Trends in Food Science & Technology, 112(1): 608-621. ‏
65. Mohammed, A., Muhammed, A., and Jumaa, M. (2026). Impact of mixing camel milk with other species milk on the physiochemical properties and sensory acceptability of yogurt. Journal of Food Science and Technology, 23(171):151-168. ‏
66. Mohammed, T. and Zewude, A. (2022). Assessment of bacteriological quality of raw bulk milk of camel, cow and goat from local markets in Yabello District, Borana Zone, Oromia Regional State. Journal Microbiological Modern Technology, 9(4): 1-10. ‏
67. Morozov, V., Hansman, G., Hanisch, F., Schroten, H. and Kunz, C. (2018). Human milk oligosaccharides as promising antivirals. Molecular Nutrition & Food Research, 62(6): e1700679. ‏
68. Motas, M., Jiménez, S., Oliva, J., Cámara, M. and Pérez-Cárceles, M. (2021). Heavy metals and trace elements in human breast milk from industrial/mining and agricultural zones of southeastern Spain. International Journal of Environmental Research and Public Health, 18(17): 1-15. ‏
69. Muhammad, S. and Tomo, F. (2023). Bacteriological and physicochemical study of retailed cow milk collected from different locations in Birnin Kebbi. Applied Science and Technology Research Journal, 2(1): 49-55.
70. Osthoff, G., Hugo, A., Madende, M., Schmidt, L., Kobeni, S., and Deacon, F. (2021). Milk composition of free-ranging impala (Aepyceros melampus) and tsessebe (Damaliscus lunatus lunatus), and comparison with other African Bovidae. Animals, 11(2): 516. ‏
71. Özdemir, D. and Kahyaoğlu, D. (2020). Identification of microbiological, physical, and chemical quality of milk from milk collection centers in Kastamonu Province. Turkish Journal of Veterinary & Animal Sciences, 44 (1): 118-130.
72. Özdemir, G. (2024). A Research on satisfaction levels of water buffalo breeders in Sivas Province, Türkiye. Journal of Tekirdag Agricultural Faculty, 21(2): 444-456.
73. Pal, U., Mandal, P., Rao, V. and Das, C. (2011). Quality and utility of goat milk with special reference to India: An overview. Asian Journal of Animal Sciences, 5(2): 56-63.
74. Polidori, P., Rapaccetti, R., Klimanova, Y., Zhang, J., Santini, G. and Vincenzetti, S. (2022). Nutritional parameters in colostrum of different mammalian species. Beverages, 8(3): 54-66.
75. Prajapati, D., Kapadiya, D., Jain, A., Mehta, B., Darji, V. and Aparnathi, K. (2017). Comparison of Surti goat milk with cow and buffalo milk for physicochemical characteristics, selected processing-related parameters and activity of selected enzymes. Veterinary World, 10(5): 477–484.
76. Prosser, C. (2021). Compositional and functional characteristics of goat milk and relevance as a base for infant formula. Journal of food Science, 86(2): 257-265.
77. Rafiq, S., Huma, N., Pasha, I., Sameen, A., Mukhtar, O. and Khan, M. (2016). Chemical composition, nitrogen fractions and amino acids profile of milk from different animal species. Asian-Australasian Journal of Animal Sciences, 29(7): 1022–1028.
78. Rahim, A., Mohammed, S. and Abbas, M. (2008). Constituents of Dorcas gazelles (Gazella dorcas) Milk. Sudan Academy of Sciences Journal, 2(1): 97-103. ‏
79. Ranvir, S., Hooda, A., Bose, A. and Kumar, H. (2023). Chemistry of Raw Milk: Composition, Distribution, and Factors. Ed(s): Megh R. G., Suvartan G. R. and Junaid A. M., Tylor and Francis Group, Apple Academic Press, United States of America.
80. Ren, Q., Liu, Y., Keijzer, P., Wichers, H. and Hettinga, K. (2025). Effect of lipid droplet size on the digestion of goat milk proteins with different casein: Whey protein ratios. Food Hydrocolloids, 163(3): 1-9. ‏
81. Roy, D., Ye, A., Moughan, P. and Singh, H. (2020a). Gelation of milks of different species (dairy cattle, goat, sheep, red deer, and water buffalo) using glucono-δ-lactone and pepsin. Journal of Dairy Science, 103(7): 5844-5862. ‏ Roy, D., Ye, A., Moughan, P. and Singh, H. (2020b). Composition, structure, and digestive dynamics of milk from different species—A review. Frontiers in Nutrition, 7(1): 1-17. ‏
82. Sabahelkhier, M., Faten, M. and Omer, F. (2012). Comparative determination of biochemical constituents between animals (goat, sheep, cow and camel) milk with human milk. Research Journal of Recent Sciences, 1(5): 69-71. ‏
83. Salhi, O., Dahmani, H., Nabi, M., Ouchetati, I., Mekri, M., Mihoubi, M. and Khelifi N. (2025). Comparative analysis of physicochemical properties and fatty acid composition of milk from different ruminant species: implications for dairy production and nutrition. Journal of the Saudi Society of Agricultural Sciences, 24(4): 1-10. ‏
84. Salih, A. and Al-Ani, M. (2014). Analysis of Iraqi Camels Milk Components. Journal of Al-Anbar University for Pure Science, 8(3): 35-40. (In Arabic)
85. Seifu, E. (2022). Recent advances on camel milk: Nutritional and health benefits and processing implications—A review. AIMS Agriculture and Food, 7(4): 777-804. ‏
86. Shitahun, Y., Endaye, M. and Kassa, A. (2025). Assessment on level of selected metals and proximate composition of raw cow milk samples from selected sites of Bahir Dar City and its surrounding. Food Chemistry Advances, 7(1): 1-8. ‏
87. Soliman, G. (2005). Comparison of chemical and mineral content of milk from human, cow, buffalo, camel and goat in Egypt. The Egyptian Journal of Hospital Medicine, 21(1): 116-130. ‏
88. Sudharani, K., Swarnalatha, G. and Rao, K. (2021). Evaluation and comparative study on the physico-chemical parameters of milk samples collected from Buffalo, cow, sheep and goat of north coastal Andhra Pradesh. The Pharmacology Innovation Journal. 10(8): 1214-1219. ‏
89. Sushila, S., Balda, A., Balhara, N., Aarushi, A. and Giri, A. (2024). Literature review and health risks assessment of heavy metal contamination in human milk. Discover Minerals, 1(1): 2 – 11.
90. Szyller, H., Antosz, K., Batko, J., Mytych, A., Dziedziak, M., Wrześniewska, M. and Pytrus, T. (2024). Bioactive components of human milk and their impact on child’s health and development, literature review. Nutrients, 16(10): 1487- 1496. ‏
91. Tamimi, N. and Al-Shihani, S. (2020). Assessment of the Milk Components of Iraqi Dromedary Camels. Basrah Journal of Veterinary Research, 19(3): 80‐89. ‏
92. Tegegn, A. (2021). Microbial safety, physical properties and chemical composition of cow milk in Ethiopia, A review. Global Journal of Animal Scientific Research, 9(1): 51-75.
93. Thannoun, A. and Abbo, A. (2010). Effect of Lactation period on the chemical and fatty acids composition of breast milk in Mosul city. Mesopotamia Journal of Agriculture, 38(1): 12-23. ‏ Uztemur, A., Orman, A., Kara, Ç. and Wronski, T. (2024). Dietary enrichment and feed preferences of threatened goitered gazelle (Gazella subgutturosa) in captivity. Applied Animal Behaviour Science, 281(2): 1-8.
94. Van Saun, R. and Wagner, D. (2025). Nutrition of Deer. In: Deer Veterinary Medicine, Ed: Foster, A. P., John Wiley and Sons Ltd., United States of America.
95. Wang, Y., Bekhit, A., Morton, J. and Mason, S. (2017). Nutritional value of deer milk. In Nutrients in Dairy and their Implications on Health and Disease. Eds: Ronald R. W., Victor R. P. and Sherma Z., Academic Press (Elsevier), Cambridge, United States of America.
96. Wilkens, M. and Muscher-Banse, A. (2020). Review: regulation of gastrointestinal and renal transport of calcium and phosphorus in ruminants. Animal, 14(1): s29–s43. ‏
97. Yadav, N., Yadav, R. and Pokhrel, B. (2018). Qualitative analysis of milk available in local market of Janakpurdham, Nepal. Tribhuvan University Journal of Microbiology, 5(2): 97-100. ‏
98. Yasmin, I., Iqbal, R., Liaqat, A., Khan, W., Nadeem, M., Iqbal, A. and Khaliq, A. (2020). Characterization and comparative evaluation of milk protein variants from Pakistani dairy breeds. Food Science of Animal Resources, 40(5): 689- 698‏.
99. Yau, D., Ummi, A. and Nura, T. (2020). Comparative study of the physicochemical parameters goat, cow and camel’s milk collected from Maigatari Town, Jigawa State, Nigeria. International Journal of Food Science and Nutrition, 5(3): 155-161. ‏
100. Zhang, Y., Zhang, X., Mi, L., Li, C., Zhang, Y., Bi, R., Pang, J. and Li, Y. (2022). Comparative proteomic analysis of proteins in breast milk during different lactation periods. Nutrients, 14(17): 1-15.
101. Zhu, Z., Zhu, X., Kong, F. and Guo, W. (2019). Quantitatively determining the total bacterial count of raw goat milk using dielectric spectra. Journal of dairy science, 102(9): 7895-7903. ‏ Zou, Y., Chen, Y., Meng, Q., Wang, Y. and Zhang, Y. (2024). Cow milk fatty acid and protein composition in different breeds and regions in China. Molecules, 29(21): 1-15.