Germination and Early Growth Performances of Mung Bean (Vigna radiata (L.) Wilczek) Genotypes Under Salinity Stress

Yıl 2020, , 318 - 328, 29.09.2020

Berk Benlioğlu , Uğur Özkan

https://doi.org/10.33462/jotaf.677216

Cited By: 5

Öz

Salinity is the abiotic stress factor that most restricts agricultural production after drought. In this study, mung beans (Vigna radiata (L.) Wilczek) genotypes were analyzed the resistance performance to salinity stress in germination stage and early seedling stage. In accordance with this purpose, 17 mung bean genotypes were used in the study. Genotypes used in the study were treated with distilled water (0) and 2 different salt doses (4 and 8 mmhos cm-1 NaCl) as the control group. Petri dishes were allowed to stay at room temperature (25 ° C) for 9 days, and salt solution was added when required. The data obtained from the study, were obtained on the average of the measurements made on the 9th day and then analyzed. Germination power, root length, shoot length, fresh weight and dry weight were determined as the analyzed parameters in the study. Increasing NaCl doses generally affected all parameters negatively. According to the analysis of variance with the results obtained, the genotypic effect was found to be statistically significant in all parameters, which was analyzed in this study. Stress dose was found to be statistically significant in all parameters except the dry weight parameter. In addition to this, genotype × stress dose interaction was determined to be statistically significant in germination power, root length and shoot length parameters. Genotypes, which were numbered No.20 and No.24, had maximum results in statistically important parameters such as germination power (97.00-94.11%), root length (1.557-1.563 cm) and shoot length (2.033-1.793 cm) under applied highest salt dose. As a result of this, No.20 and No.24 genotypes were determined to be the more tolerant to salt stress than other genotypes used in the study. As a conclusion, it is suggested that No.20 and No.24 genotypes can be used as parental plants in the breeding of new varieties tolerant to salinity in the future.

Anahtar Kelimeler

Vigna radiata (L.) Wilczek, Abiotic stress, NaCl, Germination rate, Early seedling

Kaynakça

• Abd-Alla, M.H., Vuong, T.D. and Harper, J.E. 1998. Genotypic differences in nitrogen fixation response to NaCl stress in intact and grafted soybean. Crop Science 38: 72.
• Ashraf, M. and Rasul, E. 1988. Salt tolerance of mung bean (Vigna radiata (L.) Wilczek) at two growth stages. Plant and Soil, 110(1), 63-67.
• Ashraf, Muhammad, and Majid, R. Foolad. 2013. "Crop breeding for salt tolerance in the era of molecular markers and marker‐assisted selection." Plant Breeding 132.1: 10-20.
• Bhattacharjee, A., Maity, S., Banerjee, G., Roy, M., Pal, C., Pal, B. and Chakrabarti, D. 2000. Chemical induced prolongation of seed viability and stress tolerance capacity of mung bean seedlings. Seed Science and Technology (Switzerland), 28(1), 155-162.
• Culha, S. and Cakirlar, H. 2011. The Effect of Salinity on Plants and Salt Tolerance Mechanisms. Afyon Kocatepe University Journal of Sciences and Engineering, Vol: 11(2):11-34.
• Dubey, R.S. 1985. Effect of salinity on nucleic acid metabolism of germi-nating rice seeds differing in salt tolerance, Plant Physiol. Biochemistry. Vol:12, p:9–16.
• HanumanthaRao, B., Nair, R. M. and Nayyar, H. 2016. Salinity and high temperature tolerance in mungbean [Vigna radiata (L.) Wilczek] from a physiological perspective. Frontiers in Plant Science, 7, 957.
• Hug, S.M.I., Larher, F. 1983. Osmoregulation in higher plants: effects of NaCI salinity on non-nodulated Phaseolus aureus L. I. Growth and mineral content. New phytologist. 93: 203-208.
• ISTA, 2016. International Seed Testing Association. International Rules for Seed Testing.
• Kaya, M. D., Ipek, A. and Ozturk, A. 2003. Effects of different soil salinity levels on germination and seedling growth of safflower (Carthamus tinctorius L.). Turkish Journal of Agriculture and Forestry, 27(4), 221-227.
• Maliwal, G.L. and Paliwal, K.V. 1982. Salt tolerance of some mungbean (Vigna radiata), urdbean (Vigna mungo) and gaur (Cyamopsis tetragonoloba) varieties at germination and early stages. Legume Research 5: 23-30.
• Misra, N., Murmu, B., Singh, P. and Misra, M. 1996. Growth and proline accumulation in mungebean seedlings as affected by sodium chloride. Biologia Plantarum 58: 531-536.
• Misra, N. and Dwivedi, U. N. 2004. Genotypic difference in salinity tolerance of green gram cultivars. Plant Science, 166(5), 1135-1142.
• Mohammed, A. H. M. A. 2007. Physiological aspects of mungbean plant (Vigna radiata L. Wilczek) in response to salt stress and gibberellic acid treatment. Research Journal of Agriculture and Biological Sciences, 3, 200-213.
• Moose, S. P. and Mumm, R. H. 2008. Molecular plant breeding as the foundation for 21st century crop improvement. Plant physiology, 147(3), 969-977.
• Ozgen, M., Ertunc, F., Kinaci, G., Yildiz, M., Birsin, M., Ulukan, H., Koyuncu, N. and Sancak, C. 2005. New approaches and applications in agricultural Technologies. Turkey Agricultural Engineering Technical Conference 3-7 January 2005 p.
• Paliwal, K. V. and Maliwal, G. L. 1980. Growth and nutrient uptake relationship of some crops in saline substrate. Annals of the Arid Zone 19: 251-253.
• Parida, A.K. and Das, A.B. 2005.Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety. 60, 324–349.
• Pitman, M.G. and Lauchli, A. 2002. Global Impact of Salinity and Agricultural Ecosystems. Salinity: Environment-Plants-Molecules Published by Kluwer Academic Publishers. Dordrecht. The Netherlands. 522 p.
• Promila, K. and Kumar, S. 2000. Vigna radiata seed germination under salinity. Biologia Plantarum 43: 423–426.
• Rabie, G. H. 2005. Influence of arbuscular mycorrhizal fungi and kinetin on the response of mungbean plants to irrigation with seawater. Mycorrhiza, 15(3), 225-230.
• Raptan, P. K., Hamid, A., Khaliq, Q. A., Solaiman, A. R. M., Ahmed, J. U. and Karim, M. A. 2001. Salinity Tolerance of Blackgram and Mungbean. 한국작물학회지, 46(5), 380-386.
• Saha, P., Chatterjee, P. and Biswas, A.K. 2010. NaCl pretreatment alleviates salt stress by enhancement of antioxidant defense and osmolyte accumulation in mungbean (Vigna radiata L. Wilczek). Indian Journal of Experimental Biology 48: 593-600.
• SAS, Institute Inc. 2015. JMP® Statistical Discovery Software, version 12.0; SAS Institute Inc., Cary, NC, USA, 2015.
• Sheoran, I.S. and Garg, O.P. 1978. Effect of salinity on activities of RNAse, DNAse and protease during germination and early seedling growth of mung bean, Physiologia Plantarum, Vol: 44, p:171–174.
• Siemonsma, J.S. and Na Lampang, A. 1992. Vigna radiata (L.) Wilczek. In: Plant Resources of South-East Asia 1, Pulses, Editors: van der Maesen L.J.G. and Somaatmadja S., Pudoc, Leiden, Netherlands, pp. 71-74.
• Singh, D. P. and Singh, B. B. 2011. Breeding for tolerance to abiotic stresses in mungbean. Food Legumes, 24(2), 83-90.
• Tuteja, N. 2007. Mechanisms of High Salinity Tolerance in Plants. Methods in Enzymology, 428: 419-438.
• Yıldız, M. and Ozgen, M. 2004. The effect of a submersion pretreatment on in vitro explant growth and shoot regeneration from hypocotyls of flax (Linum usitatissimum). Plant Cell, Tissue and Organ Culture, 77(1):111-115.
• Zhu J.K. 2002. Salt and Drought Stress Signal Transductıon in Plants. Annual Review of Plant Biology. 53:247-273.
• Cakmakci, S. and Dallar, A. 2019. Farklı Sıcaklık ve Tuz Konsantrasyonlarının Bazı Silajlık Mısır Çeşitlerinin Çimlenme Özellikleri Üzerine Etkileri. Tekirdağ Ziraat Fakültesi Dergisi, 16(2), 121-132.
• Demirbas, S. and Balkan, A. 2018. Tuz Stresi Koşullarında Bazı Tritikale Çeşitlerinin Hidrojen Peroksit Ön Uygulamasına Tepkileri. Tekirdağ Ziraat Fakültesi Dergisi, 15(2), 5-13.