CIDR Uygulanan Kıvırcık ve Karacabey Merinosu Koyunlarda T3, T4 ve Kortizol Düzeylerinin Belirlenmesi

Yıl 2018, Cilt: 15 Sayı: 2, 112 - 118, 15.05.2018

Şeniz Öziş Altınçekiç Mehmet Koyuncu Serdar Duru

Öz

Bu çalışma, östrus senkronizasyonunu
sağlamak amacıyla kullanılan CIDR uygulamasının, Kıvırcık ve Karacabey Merinosu
koyunlarında uygulama esnasında bir stres kaynağı olup olmadığının belirlenmesi
amacıyla yürütülmüştür. Bu amaçla koyunlara CIDR aparatlarının takıldığı 1. gün,
7. gün ve çıkarıldığı 14. gün alınan kan örneklerinde T3 (triiodothyronine), T4
(thyroxine) ve kortizol hormonu seviyeleri ölçülmüştür. Bu çalışmada 40 baş
Kıvırcık ve 40 baş Karacabey Merinosu olmak üzere toplam 80 baş koyun
kullanılmıştır. Koyunların boyun toplardamarından her üç dönemde 10 ml kan
EDTA’lı tüplere alınarak ticari RIA kitleri ile T3, T4 ve kortizol konsantrasyonları
belirlenmiştir. Her üç dönemde alınan kan örneklerinde T3, T4 ve kortizol
hormonu ortalama değerleri Kıvırcık koyunlarında sırasıyla 1.76 (ng/mL), 7.92
(µg/dL), 5.30 (µg/dL);
Karacabey Merinosunda ise sırasıyla 1.69 (ng/mL), 7.00 (µg/dL) ve 4.19 (µg/dL)
olarak bulunmuştur. T4 ve kortizol bakımından ırklar arasındaki farklılık önemli
(P<0.01) bulunurken,  dönemler arasındaki
farklılık sadece T3 hormonu için önemli bulunmuştur (P<0.01).

Anahtar Kelimeler

Kıvırcık, Karacabey Merinosu, stres, CIDR, T3, T4

Determination of T3, T4 and Cortisol Levels in CIDR applied Kıvırcık and Karacabey Ewes

Öz

This study has been conducted in order to determine whether the CIDR
application on Kıvırcık and Karacabey Merino ewes used for supplying estrous
synchronization a stress factor or not. For this purpose; T3
(triiodothyronine), T4 (thyroxine) and cortisol hormone levels of sheep in
their blood samples were measured on day 1 when CIDR apparatus were mounted, on
day 7 and on day 14 when they were removed. In this study, 80 head of ewes in
total were used which consisted of 40 head Kıvırcık and 40 head Karacabey
Merino ewes. 10 ml of blood from jugular veins of sheep were taken at the
specified periods into EDTA tubes, and their T3, T4 and cortisol concentrations
were identified by commercial RIA kits. In Kıvırcık race, these values were
identified as 1.76 (ng/mL), 7.92 (µg/dL), 5.30 (µg/dL) respectively while in
Karacabey Merino race as 1.69 (ng/mL), 7.00 (µg/dL) and 4.19 (µg/dL)
respectively. In terms of T4 and cortisol, the difference between races were
found significant (P<0.01) while difference between the periods were found
significant only for T3 hormone (P<0.01).

Anahtar Kelimeler

Kivircik sheep, Karacabey Merino, stress, CIDR, T3, T4

Kaynakça

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