Yıl 2019, Cilt 16 , Sayı 1, Sayfalar 11 - 22 2019-01-25

An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period
Origanum onites Tentürü Üretim Şartlarının Tepki Yüzey Yöntemi ile Optimizasyonu: Katı/Sıvı Oranı, Etanol Konsantrasyonu ve Depolama Süresinin Etkisi

Mahmut KAPLAN [1] , Mustafa Mücahit YILMAZ [2] , Rabia USLU [3] , Serap KÖPRÜ [4] , Hürmet GÖZELLE [5] , Gamze MUHDEREM [6]


In this study, Origanum onites was used for the production of tincture, a hydroalcholic extract, and manufacturing conditions

were optimized to determine the processing factor variable levels to increase the final quality of samples. For this purpose,

response surface methodology study was applied and Box Behnken design was used for the optimization. Three factors were

selected namely liquid/solid ratio, ethanol concentration and storage period for the preparation of samples. Total phenolic

content, antiradical activity and efficient of concentration (EC50) values were determined to evaluate the bioactivity of tincture

samples. It was observed that the total phenolic content of the tincture samples was affected by ethanol concentration and

liquid/solid ratio. Total phenolic contents of tincture samples were in the range of 964.1-7020.8 mg GAE/ L samples while the

efficient concentration (EC50) ranged from 44.88 to 89.56. Increase of solid level increased the bioactivity values of samples,

but this increment decreased the tincture yield significantly. Optimization results showed that the highest total phenolic content

would be at 50% ethanol concentration, 6 ml ethanol level which means the liquid/solid ratio is 1:5 and 15 days storage for the

tincture samples.

Bu çalışmada, bir hidroalkolik ekstrakt tipi olan tentür üretimi amacıyla Origanum onites bitkisi kullanılmış ve son ürün

kalitesini artırmaya yönelik olarak üretim şartlarını optimize etmek üzere proses faktör seviyelerinin belirlenmesi amaçlanmıştır.

Bu amaçla, optimizasyon işlemi için tepki yüzey yöntemi tabanlı Box-Behnken deneme tasarımından faydalanılmıştır.

Örneklerin hazırlanması amacıyla sıvı/katı oranı, etanol konsantrasyonu ve depolama süresi faktör olarak seçilmiştir. Tentür

örneklerinin biyoaktif özelliklerinin belirlenmesi amacıyla toplam fenolik madde miktarı, antiradikal kapasite ve EC50 değeri

hesaplanmıştır.Sıvı/katı oranı ve etanol konsantrasyonunun örneklerin toplam fenolik madde miktarı üzerinden etkili olduğu

görülmüştür. Örneklerin toplam fenolik madde değerleri 964.1-7020.8 mg GAE/ L, EC50 değerleri ise 44.88-89.56 aralığında

değişim göstermiştir. Örnek konsantrasyonunun artışı toplam fenolik madde miktarını artırırken, bu artış tentür veriminde

düşüşe yol açmıştır. Optimizasyon sonuçları en yüksek toplam fenolik madde miktarının, %50 etanol konsantrasyonunda, 1:5

katı/sıvı oranında, 15 günlük depolama süresi sonunda elde edilebileceğini göstermiştir.

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Birincil Dil en
Konular Fen
Bölüm Makaleler
Yazarlar

Yazar: Mahmut KAPLAN (Sorumlu Yazar)
Ülke: Turkey


Yazar: Mustafa Mücahit YILMAZ
Ülke: Turkey


Yazar: Rabia USLU
Ülke: Turkey


Yazar: Serap KÖPRÜ
Ülke: Turkey


Yazar: Hürmet GÖZELLE

Yazar: Gamze MUHDEREM

Tarihler

Başvuru Tarihi : 1 Şubat 18
Kabul Tarihi : 22 Şubat 18
Yayımlanma Tarihi : 25 Ocak 2019

Bibtex @araştırma makalesi { jotaf392334, journal = {Tekirdağ Ziraat Fakültesi Dergisi}, issn = {1302-7050}, eissn = {2146-5894}, address = {}, publisher = {Namık Kemal Üniversitesi}, year = {2019}, volume = {16}, pages = {11 - 22}, doi = {10.33462/jotaf.516734}, title = {An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period}, key = {cite}, author = {KAPLAN, Mahmut and YILMAZ, Mustafa Mücahit and USLU, Rabia and KÖPRÜ, Serap and GÖZELLE, Hürmet and MUHDEREM, Gamze} }
APA KAPLAN, M , YILMAZ, M , USLU, R , KÖPRÜ, S , GÖZELLE, H , MUHDEREM, G . (2019). An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period. Tekirdağ Ziraat Fakültesi Dergisi , 16 (1) , 11-22 . DOI: 10.33462/jotaf.516734
MLA KAPLAN, M , YILMAZ, M , USLU, R , KÖPRÜ, S , GÖZELLE, H , MUHDEREM, G . "An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period". Tekirdağ Ziraat Fakültesi Dergisi 16 (2019 ): 11-22 <https://dergipark.org.tr/tr/pub/jotaf/issue/42795/392334>
Chicago KAPLAN, M , YILMAZ, M , USLU, R , KÖPRÜ, S , GÖZELLE, H , MUHDEREM, G . "An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period". Tekirdağ Ziraat Fakültesi Dergisi 16 (2019 ): 11-22
RIS TY - JOUR T1 - An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period AU - Mahmut KAPLAN , Mustafa Mücahit YILMAZ , Rabia USLU , Serap KÖPRÜ , Hürmet GÖZELLE , Gamze MUHDEREM Y1 - 2019 PY - 2019 N1 - doi: 10.33462/jotaf.516734 DO - 10.33462/jotaf.516734 T2 - Tekirdağ Ziraat Fakültesi Dergisi JF - Journal JO - JOR SP - 11 EP - 22 VL - 16 IS - 1 SN - 1302-7050-2146-5894 M3 - doi: 10.33462/jotaf.516734 UR - https://doi.org/10.33462/jotaf.516734 Y2 - 0018 ER -
EndNote %0 Tekirdağ Ziraat Fakültesi Dergisi An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period %A Mahmut KAPLAN , Mustafa Mücahit YILMAZ , Rabia USLU , Serap KÖPRÜ , Hürmet GÖZELLE , Gamze MUHDEREM %T An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period %D 2019 %J Tekirdağ Ziraat Fakültesi Dergisi %P 1302-7050-2146-5894 %V 16 %N 1 %R doi: 10.33462/jotaf.516734 %U 10.33462/jotaf.516734
ISNAD KAPLAN, Mahmut , YILMAZ, Mustafa Mücahit , USLU, Rabia , KÖPRÜ, Serap , GÖZELLE, Hürmet , MUHDEREM, Gamze . "An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period". Tekirdağ Ziraat Fakültesi Dergisi 16 / 1 (Ocak 2019): 11-22 . https://doi.org/10.33462/jotaf.516734
AMA KAPLAN M , YILMAZ M , USLU R , KÖPRÜ S , GÖZELLE H , MUHDEREM G . An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period. Tekirdağ Ziraat Fakültesi Dergisi. 2019; 16(1): 11-22.
Vancouver KAPLAN M , YILMAZ M , USLU R , KÖPRÜ S , GÖZELLE H , MUHDEREM G . An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period. Tekirdağ Ziraat Fakültesi Dergisi. 2019; 16(1): 22-11.