The Correlation of Some Nutrient Elements and Antibacterial Activity of the Basil (Ocimum basilicum)

Yıl 2020, Cilt: 17 Sayı: 3, 381 - 391, 29.09.2020

Muazzez Gürgan Eser Sevinc Adiloglu

https://doi.org/10.33462/jotaf.699792

Cited By: 4

Öz

This study aimed to analyze the correlation of some macro and micro nutritional elements of the basil plant (Ocimum basilicum) with the antibacterial activity of the basil extract on two Gram positive and two Gram negative bacteria. The extract of the basil plants grown in laboratory conditions was obtained by solid-liquid extraction with ethanol after drying and burning of the freshly harvested above ground parts. The macro (P, K, Ca, and Mg), micro (Fe, Cu, Zn, Mn, and B) nutrients and a functional element (Al) amounts were detected by ICP-OES. The results revealed that the basil extract was compatible with herbaceous perennial plants in terms of macro nutrient elements P, K, Ca and Mg. Moreover, some micronutrients (Fe, Mn, Zn, B) and the functional element Al was found to be significantly high. The basil extract was significantly effective on inhibiting the Gram negative E. coli, P. aeruginosa, and Gram positive S. aureus and E. faecium. The element B was found to be positively correlated with the antibacterial activity of the basil plant suggesting as the B content increases, the antibacterial activity of basil extract is supposed to increase, too. The P and K contents were found to be positively correlated with antibacterial activity, while Ca and Mg contents were negatively correlated. The correlation analysis of the nutritional elements, this extract can be suggested to be more preferrable against Gram positive bacteria, especially when the Fe, Cu, Zn, Mn levels are high. These results revealed the importance of the macro and micro nutrient contents of medicinal plants which can be used traditionally against various diseases. In basil cultivation the soils low in lime may convert the negative correlation of Ca and Mg into a positive correlation in terms of antibacterial effect. Conscious and proper fertilization therefore is suggested to be effective on antibacterial activity of the basil plant.

Anahtar Kelimeler

Ocimum basilicum, Antibacterial activity, Macro elements, Micro elements, Correlation

Kaynakça

• Adiloglu, A., & Adiloglu, S. (2006). The Effect of Boron (B) Application on the Growth and Nutrient Contents of Maize in Zinc (Zn) Deficient Soil. Bulgarian Journal of Agricultural Science (Vol. 12).
• Bais, H. P., Walker, T. S., Schweizer, H. P., & Vivanco, J. M. (2002). Root specific elicitation and antimicrobial activity of rosmarinic acid in hairy root cultures of Ocimum basilicum. Plant Physiology and Biochemistry, 40(11), 983–995. http://doi.org/10.1016/S0981-9428(02)01460-2
• Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79. http://doi.org/10.1016/J.JPHA.2015.11.005
• Çalişkan, B., & Çalişkan, A. C. (2018). Potassium Nutrition in Plants and Its Interactions with Other Nutrients in Hydroponic Culture. In Potassium - Improvement of Quality in Fruits and Vegetables Through Hydroponic Nutrient Management. InTech. http://doi.org/10.5772/intechopen.71951
• Delcour, A. H. (2009, May). Outer membrane permeability and antibiotic resistance. Biochimica et Biophysica Acta - Proteins and Proteomics. NIH Public Access. http://doi.org/10.1016/j.bbapap.2008.11.005
• Dobrin, A., Ivan, E. Ş., Jerca, I. O., Bera, I.-R., Ciceoi, R., & Samih, A. A. (2018). The Accumulation of Nutrients and Contaminants in Aromatic Plants Grown in a Hydroponic System. “Agriculture for Life, Life for Agriculture” Conference Proceedings, 1(1), 284–289. http://doi.org/10.2478/alife-2018-0042
• Dumbravă, D.-G., Moldovan, C., Raba, D.--I., & Popa, M.-V. (2012). Vitamin C, chlorophylls, carotenoids and xanthophylls content in some basil (Ocimum basilicum L.) and rosemary (Rosmarinus officinalis L.) leaves extracts (Vol. 18). Retrieved from http://
• Estetlili, Bihter Colak Pekcan, Tulin , Cobanoglu, Ozgur, Aydogdu, Erol, Turan, Sevim, Anac, D. (2014). Bazı Tıbbi ve Aromatik Bitkilerin Ağır Metal ve Temel Besin Elementi İçerikleri Essential Plant Nutrients and Heavy Metals Concentrations of Some Medicinal and Aromatic Plants. Journal of Agricultural Sciences, 20, 239–247.
• IBM. (2016). IBM SPSS Statistics Version 21.
• Johansen, C., Edwards, D. G., & Loneragan, J. F. (1968). Interactions Between Intact Barley Plants. Potassium and Calcium in Their Absorption by I. Effects of Potassium on Calcium Absorption’. Plant Physiol (Vol. 43). Retrieved from www.plantphysiol.org
• Kacar, B., & İnal, A. (2010). Plant Analysis. (N. Yayınevi, Ed.). Ankara.
• Kurniawan, S. B., Purwanti, I. F., & Titah, H. S. (2018). The effect of pH and aluminium to bacteria isolated from aluminium recycling industry. Journal of Ecological Engineering, 19(3), 154–161. http://doi.org/10.12911/22998993/86147
• Lajayer, H. A., Hadian, J., MOTESHAREZADEH, B., & GHORBANPOUR, M. (2014). Assessing different levels of zinc and copper impacts on micro- and macro elements accumulation and translocation in root , shoot , infusion and decoction of basil ( Ocimum basilicum ... IMPACTS ON MICRO- AND MACRO ELEMENTS. South Western Journal of Horticulture, Biology and Environment, 5(2), 105–123.
• Mills H. A. and Jones J. B. Jr. (1996). Plant Analysis Handbook II: A practical sampling, preparation, analysis, and interpretation guide. MicroMacro Publishing.
• Moradali, M. F., Ghods, S., & Rehm, B. H. A. (2017, February 15). Pseudomonas aeruginosa lifestyle: A paradigm for adaptation, survival, and persistence. Frontiers in Cellular and Infection Microbiology. Frontiers Research Foundation. http://doi.org/10.3389/fcimb.2017.00039
• Okmen, G., & Balpınar, N. (2018). Cumhuriyet Science Journal CSJ. Cumhuriyet Science Journal, 39(3), 573–580. http://doi.org/http://dx.doi.org/10.17776/csj.356185
• Purushothaman, B., Prasannasrinivasan, R., Suganthi, P., Ranganathan, B., Gimbun, J., & Shanmugam, K. (2018). A comprehensive review on Ocimum basilicum. Journal of Natural Remedies, 18(3), 71–85. http://doi.org/10.18311/jnr/2018/21324
• Sayin, Z., Ucan, U. S., & Sakmanoglu, A. (2016). Antibacterial and Antibiofilm Effects of Boron on Different Bacteria. Biological Trace Element Research, 173(1), 241–246. http://doi.org/10.1007/s12011-016-0637-z
• Shweash, M., Abdulhameed, A., Farhan, Y. M., & Nasser, S. J. (2014). Antimicrobial activity of ethanolic extract of leaves sweet basil ( Ocimum basilicum ) against diarrhea caused by Escherichia coli in vitro. International Journal of Science and Nature, 5(4), 713–718.
• Soetan, K. O., Olaiya, C. O., & Oyewole, O. E. (2010). The importance of mineral elements for humans, domestic animals and plants: A review. African Journal of Food Science, 4(5), 200–222. Retrieved from http://www.academicjournals.org/ajfs
• Suppakul, P., Miltz, J., Sonneveld, K., & Bigger, S. W. (2003, May 21). Antimicrobial properties of basil and its possible application in food packaging. Journal of Agricultural and Food Chemistry. American Chemical Society . http://doi.org/10.1021/jf021038t
• Toval, F., Köhler, C.-D., Vogel, U., Wagenlehner, F., Mellmann, A., Fruth, A., … Dobrindt, U. (2014). Characterization of Escherichia coli isolates from hospital inpatients or outpatients with urinary tract infection. Journal of Clinical Microbiology, 52(2), 407–18. http://doi.org/10.1128/JCM.02069-13
• Xie, Y., & Yang, L. (2016). Calcium and Magnesium Ions Are Membrane-Active against Stationary-Phase Staphylococcus aureus with High Specificity. Scientific Reports, 6. http://doi.org/10.1038/srep20628