Fitobiyotik Takviyesinin Etlik Piliçlerde Büyüme Performansı, Kazanılmış Bağışıklık, Kan Metabolitleri ve Besin Sindirilebilirliği Üzerine Etkisi

Yıl 2025, Cilt: 22 Sayı: 4, 1100 - 1110, 03.10.2025

Saim Arif , Muhammad Shoaib , Safdar Hassan , Hasan Ersin Şamlı , Waseem Abbas , Muhammad Asnan , Muhammad Yousaf , Ammara Ameer , Shah Nawaz , Muhammad Sharif , Qaswar Hafeez , M. Mahboob Ali Hamid

https://doi.org/10.33462/jotaf.1748013

Öz

Bu çalışmada, Allium sativum, Calendula officinalis, Echinacea purpurea, Emblica officinalis, Ocimum sanctum, Amerikan ginsengi tozu ve Withania somnifera içeren fitobiyotik takviyesinin (FT) broylerlerde büyüme performansı, kazanılmış bağışıklık, kan metabolitleri, karkas özellikleri ve besin madde sindirilebilirliği üzerindeki etkinliğini araştırılmıştır. Toplam 260 adet günlük yaşta Ross-308 etlik civciv, beş farklı muamele grubuna rastgele dağıtılmıştır: T0 (0 mL L⁻¹ FT, kontrol), T1 (0.75 mL L⁻¹), T2 (1.0 mL L⁻¹), T3 (1.25 mL L⁻¹) ve T4 (1.5 mL L⁻¹) olacak şekilde içme suyuna FT ilave edilmiştir. Büyüme performansı haftalık olarak kaydedilmiştir. Denemenin 21. ve 35. günlerinde, her tekrardan iki adet piliç kesilerek antikor titreleri ve bağışıklık organı indeksleri (timus, bursa ve dalak) değerlendirilmiştir. 35. günde ise kan metabolitleri, karkas özellikleri, besin sindirilebilirliği ve kemik mineralizasyonunu belirlemek üzere örnekler toplanmıştır. Elde edilen veriler tamamıyla şansa bağlı deneme desenine göre varyans analizi (ANOVA) ile değerlendirilmiştir. Fitobiyotik takviyesi, kontrol grubuna (T0) kıyasla tüm muamele gruplarında yem tüketimi, canlı ağırlık artışı ve yem dönüşüm oranını (FCR) anlamlı düzeyde (P<0.05) iyileştirmiştir. T2 grubunda Newcastle hastalığı (ND) ve enfeksiyöz bronşit (IB) virüslerine karşı antikor titreleri ile bağışıklık organı indeksleri, 21. ve 35. günlerde anlamlı derecede (P<0.05) artmıştır. Ayrıca, FT takviyesi 35. günde glikoz, kolesterol, kan üre azotu (BUN), besin sindirilebilirliği ve kemik mineralizasyonu üzerinde de anlamlı (P<0.05) etkiler göstermiştir. Karkas özellikleri tüm FT gruplarında benzer bulunmuş olup, en yüksek randıman T2 grubunda, en düşük ise T1 grubunda gözlemlenmiştir. Sonuç olarak, fitobiyotik takviyesinin etlik piliçlerde antibiyotik büyüme faktörlerine (AGP) alternatif olarak kullanılabileceği ve bağışıklık ile büyüme performansında iyileşme sağlayabileceği görülmüştür.

Anahtar Kelimeler

Antibiyotik alternatifleri , Fitobiyotik , Broyler , Besin sindirilebilirliği , Karkas özellikleri

Etik Beyan

All experimental procedures were approved by the Graduate Studies and Research Board Committee of the University of Agriculture Faisalabad prior to the commencement of the trial (Dated: 08-04-2024, numbered: IADS/2024-350).

Destekleyen Kurum

University of Agriculture, Faisalabad

Teşekkür

The authors would like to thank the Institute of Animal and Dairy Sciences, Faculty of Animal Husbandry, University of Agriculture, Faisalabad, for providing research facilities, and Bin Ibrahim Pharma Pvt. Ltd., Pakistan, for their collaboration and support in strengthening industry academia linkages.

Effect of Phytobiotics Supplementation on Growth Performance, Acquired Immunity, Blood Metabolites and Nutrient Digestibility in Broiler Chickens

Öz

The aim of this experiment was to investigate the efficacy of phytobiotic supplementation (PS), consisting on Allium sativum, Calendula officinalis, Echinacea purpurea, Emblica officinalis, Ocimum sanctum, powdered American ginseng, Withania somnifera active ingredients, on growth performance, acquired immunity, blood metabolites, carcass characteristics and nutrient digestibility of broiler birds. Two hundred and sixty, day-old broiler chicks (Ross-308) were divided into five treatments (T0; 0, T1; 0.75, T2; 1, T3; 1.25 and T4; 1.5 mL L-1 concentrations of PS in drinking water, respectively. Broiler growth performance was recorded weekly. Two birds from each replicate were slaughtered on day 21st and 35th to evaluate antibody titers, immune organ index (thymus, bursa and spleen). Samples for blood metabolites, carcass characteristics, nutrient digestibility and bone mineralization were collected on the 35th day. Collected data was analyzed using analysis of variance under a completely randomized design. PS had improved (P<0.05) feed intake, weight gain and feed conversion ratio (FCR) among all the treatments compared with T0. Similarly, antibody titers for Newcastle disease (ND) and infectious bronchitis (IB) viruses in T2, immune organ index were significant (P<0.05) on 21st and 35th day of the experiment. PS had also significant (P<0.05) effect on glucose, cholesterol, blood urea nitrogen (BUN), nutrient digestibility and bone mineralization on the 35th day. Carcass characteristics were same for PS treatments, but the highest dressing percentage was observed in T2 and lowest in T1. Based on the results, the PS can be an alternative to antibiotic growth promoters (AGPs) with a better acquired immunity and growth performance in broilers.

Anahtar Kelimeler

Antibiotics alternatives , Phytobiotic , Broilers , Nutrient digestibility , Carcass characteristics

Etik Beyan

All experimental procedures were approved by the Graduate Studies and Research Board Committee of the University of Agriculture Faisalabad prior to the commencement of the trial (Dated: 08-04-2024, numbered: IADS/2024-350).

Destekleyen Kurum

University of Agriculture, Faisalabad

Teşekkür

The authors would like to thank the Institute of Animal and Dairy Sciences, Faculty of Animal Husbandry, University of Agriculture, Faisalabad, for providing research facilities, and Bin Ibrahim Pharma Pvt. Ltd., Pakistan, for their collaboration and support in strengthening industry academia linkages.

Kaynakça

• Amad, A., Männer, K., Wendler, K., Neumann, K. and Zentek, J. (2011). Effects of a phytogenic feed additive on growth performance and ileal nutrient digestibility in broiler chickens. Poultry Science, 90: 2811-2816.
• Amouzmehr, A., Dastar, B., Nejad, J. G., Sung, K. I., Lohakare, J. and Forghani, F. (2012). Effects of garlic and thyme extracts on growth performance and carcass characteristics of broiler chicks. Journal of Animal Science and Technology, 54: 185-190.
• AOAC. (2000). Official Methods of Analysis (17th ed.): Association of Official Analytical Chemists. Arif, M. S., Hamid, M. M. A., Raheem, A. and Khalil, M. Z. (2024). Phytobiotics as an alternative to antibiotics in poultry. Biological Times, 3: 21-22.
• Begum, K., Talukdar, J. K., Kalita, K. P., Roy, T. C., Nath, R. and M., R. (2019). Effect of dietary supplementation of gooseberry/amla (Emblica officinalis) powder on the performance of commercial broiler chicken. International Journal of Livestock Research, 9: 95-102.
• Biondo, P. B. F., Carbonera, F., Zawadzki, F., Chiavelli, L. U., Pilau, E. J., Prado, I. N. and Visentainer, J. V. (2017). Antioxidant capacity and identification of bioactive compounds by GC-MS of essential oils from spices, herbs and citrus. Current Bioactive Compounds, 13: 137-143.
• Cho, J., Kim, H. and Kim, I. (2014). Effects of phytogenic feed additive on growth performance, digestibility, blood metabolites, intestinal microbiota, meat color and relative organ weight after oral challenge with Clostridium perfringens in broilers. Livestock Science, 160: 82-88.
• Choi, I., Park, W. and Kim, Y. (2010). Effects of dietary garlic powder and α-tocopherol supplementation on performance, serum cholesterol levels, and meat quality of chicken. Poultry Science, 89: 1724-1731.
• Chowdhury, S., Mandal, G. P. and Patra, A. K. (2018a). Different essential oils in diets of chickens: 1. Growth performance, nutrient utilisation, nitrogen excretion, carcass traits and chemical composition of meat. Animal Feed Science and Technology, 236: 86-97.
• Chowdhury, S., Mandal, G. P., Patra, A. K., Kumar, P., Samanta, I., Pradhan, S. and Samanta, A. K. (2018b). Different essential oils in diets of broiler chickens: 2. Gut microbes and morphology, immune response and some blood profile and antioxidant enzymes. Animal Feed Science and Technology, 236: 39-47.
• Darabighane, B., Mahdavi, A., Aghjehgheshlagh, F. M., Zarei, A., Kasapidou, E. and Nahashon, S. N. (2017). Effect of Aloe vera and vitamin E supplementation on the immune response of broilers. Colombian Journal of Animal Science and Veterinary Medicine, 30: 159-164.
• Dourado, L. R. B., Siqueira, J. C., Sakomura, N. K., Pinheiro, S. R. F., Marcato, S. M., Fernandes, J. B. K. and Silva, J. H. V. (2010). Poultry feed metabolizable energy determination using total or partial excreta collection methods. Brazilian Journal of Poultry Science, 12: 129-132.
• Fotea, L., Costăchescu, E., Hoha, G. and Leonte, D. (2010). The effect of oregano essential oil (Origanum vulgare L.) on broiler performance. Lucrări Ştiinţifice Seria Zootehnie, 53: 253-256.
• Gaikwad, D., Fulpagare, Y., Bhoite, U., Deokar, D. and Nimablkar, C. (2019). Effect of dietary supplementation of ginger and cinnamon on growth performance and economics of broiler production. International Journal of Current Microbiology and Applied Sciences, 8: 1849-1857.
• Gopi, M., Karthik, K., Manjunathachar, H. V., Tamilmahan, P., Kesavan, M., Dashprakash, M., Balaraju, B. L. and Purushothaman, M. R. (2014). Essential oils as a feed additive in poultry nutrition. Advances in Animal and Veterinary Sciences, 2: 1-7.
• Greene, E. S., Maynard, C., Owens, C. M., Meullenet, J. F. and Dridi, S. (2021). Effects of herbal adaptogen feed-additive on growth performance, carcass parameters, and muscle amino acid profile in heat-stressed modern broilers. Frontiers in Physiology, 12: 784952.
• Hafeez, A., Shah, S. A. A., Khan, R. U., Ullah, Q. and Naz, S. (2020). Effect of diet supplemented with phytogenics and protease enzyme on performance, serum biochemistry and muscle histomorphology in broilers. Journal of Applied Animal Research, 48: 326-330.
• Hanieh, H., Narabara, K., Piao, M., Gerile, C., Abe, A. and Kondo, Y. (2010). Modulatory effects of two levels of dietary Alliums on immune response and certain immunological variables, following immunization, in White Leghorn chickens. Animal Science Journal, 81: 673-680.
• Hashemi, S., Zulkifli, I., Bejo, M. H., Farida, A. and Somchit, M. (2008). Acute toxicity study and phytochemical screening of selected herbal aqueous extract in broiler chickens. International Journal of Pharmacology, 4: 352-360.
• Javandel, F., Navidshad, B., Seifdavati, J., Pourrahimi, G. and Baniyaghoub, S. (2008). The favorite dosage of garlic meal as a feed additive in broiler chickens ratios. Pakistan Journal of Biological Sciences, 11: 1746-1749.
• Keskin, B. and Güneş, E. (2023). The Effects of information pollution on poultry companies: The case of Turkey. Journal of Tekirdag Agricultural Faculty, 20(2): 387-398.
• Khaligh, F., Sadeghi, G., Karimi, A. and Vaziry, A. (2011). Evaluation of different medicinal plants blends in diets for broiler chickens. Journal of Medicinal Plants Research, 5: 1971-1977.
• Khan, S. (2014). Garlic (Allium sativum) modulated serum cholesterol level, improved immune status and carcass yield of broilers. Pakistan Journal of Science, 66: 171-174.
• Kharde, K. R. and Soujanya, S. (2014). Effect of garlic and neem leaf powder supplementation on growth performance and carcass traits in broilers. Veterinary World, 7: 799-802.
• Kim, B. C., Ryu, Y. C., Cho, Y. J. and Rhee, M. S. (2006). Influence of dietary α-tocopheryl acetate supplementation on cholesterol oxidation in retail packed chicken meat during refrigerated storage. Bioscience, Biotechnology, and Biochemistry, 70: 808-814.
• Lee, K., Lee, K., Kim, G., Kim, J., Yeon, J., Cho, S., Chang, B. and Kim, S. (2016). Effects of dietary fermented garlic on the growth performance, relative organ weights, intestinal morphology, cecal microflora and serum characteristics of broiler chickens. Brazilian Journals in Veterinary Medicine and Animal Science, 18: 511-518.
• Mangisah, I., Suthama, N. and Rizqiati, H. (2020). Feeding combination of Lactobacillus casei and extracts of dahlia tuber or garlic on intestinal bacteria, nutrients digestibility and performance of broiler chickens. Indonesian Journal of Animal Science, 30: 158-166.
• Martel, J., Ojcius, D. M., Ko, Y. F. and Young, D. (2020). Phytochemicals as prebiotics and biological stress inducers. Trends in Biochemical Sciences, 45: 462-471.
• Masoud-Moghaddam, S., Mehrzad, J., Ghamsari, A. A., Kashani, R. B. and Saeidi, J. (2021). Comparison of herbal additives effects with growth-promoting antibiotic on productive traits, biochemical parameters and serum hepatic enzymes of broilers. Iranian Journal of Medicinal and Aromatic Plants Research, 37: 412-423.
• Nahed, A., Abd El-Hack, M. E., Albaqami, N. M., Khafaga, A. F., Taha, A. E., Swelum, A. A., El-Saadony, M. T., Salem, H. M., El-Tahan, A. M. and AbuQamar, S. F. (2022). Phytochemical control of poultry coccidiosis: A review. Poultry Science, 101: 101542.
• Nahed, A., Shewita, R. S., Abd El-Hack, M. E., AlKahtane, A., Alarifi, S., Alkahtani, S., Abdel-Daim, M. M. and Sedeik, M. E. (2020). Effect of essential oils on the immune response to some viral vaccines in broiler chickens, with special reference to Newcastle disease virus. Poultry Science, 99: 2944-2954.
• Oluwafemi, R., Lawal, A. O., Adetope, A. S. and Alagbe, J. (2021). Effects of dietary inclusion of ginger (Zingiber officinale) and garlic (Allium sativum) oil (GGO) mixtures on carcass characteristics and sensory evalaution of broiler chickens. Journal La Medihealtico, 2: 22-31.
• Onu, P. N. (2010). Evaluation of two herbal spices as feed additives for finisher broilers. Biotechnology in Animal Husbandry, 26: 383-392.
• Panda, A., Rao, S. R., Raju, M. and Sunder, G. S. (2009). Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Australasian Journal of Animal Sciences, 22: 1026-1031.
• Prasad, R., Rose, M., Virmani, M., Garg, S. and Puri, J. (2009). Lipid profile of chicken (Gallus domesticus) in response to dietary supplementation of garlic (Allium sativum). International Journal of Poultry Science, 8: 270-276.
• Rafeeq, M., Rashid, N., Tariq, M. M., Sheikh, I. S., Mustafa, M. Z., Shafee, M., Mehmood, K., Bilal, R. M. and Asmat, T. (2021). Effect of herbal extract on the growth performance, serum biochemical composition, ileal histo-morphology and immune response of broiler chickens. Pakistan Journal of Zoology, 53: 1793.
• Rahimi, S., Zadeh, Z. T., Torshizi, K., Omidbaigi, R. and Rokni, H. (2011). Effect of the three herbal extracts on growth performance, immune system, blood factors and intestinal selected bacterial population in broiler chickens. Journal of Agricultural Science and Technology, 13: 527-539.
• Rozbeh, F., Ali, K. and Arash, A. (2013). A review of the role of five kinds of alternatives to in-feed antibiotics in broiler production. Journal of Veterinary Medicine and Animal Health, 5: 317-321.
• Safiullah, Chand, N., Khan, R. U., Naz, S., Ahmad, M. and Gul, S. (2019). Effect of ginger (Zingiber officinale Roscoe) and organic selenium on growth dynamics, blood melanodialdehyde and paraoxonase in broilers exposed to heat stress. Journal of Applied Animal Research, 47: 212-216.
• Sharif, M., Shoaib, M., Rahman, M. A. U., Ahmad, F. and Rehman, S. U. (2018). Effect of distillery yeast sludge on growth performance, nutrient digestibility and slaughter parameters in Japanese quails. Scientific Reports, 8: 1-6.
• Steel, R. G. D., Torrie, J. H. and Dickie, D. A. (1997). Principles and Procedures of Statistics. A Biometric Approach, 3rd edition, McGraw-Hill, Book Publishing Company, Toronto, Canada.
• Su, G., Zhou, X., Wang, Y., Chen, D., Chen, G., Li, Y. and He, J. (2020). Dietary supplementation of plant essential oil improves growth performance, intestinal morphology and health in weaned pigs. Journal of Animal Physiology and Animal Nutrition, 104: 579-589.
• Talebi, E. and Jahromi, M. H. (2023). Effect of Olive Leaves Hydroalcoholic Extract (Olea Europaea L.) and LactoFeed® probiotics on Induced Ascites in Male Broilers. Journal of Tekirdag Agricultural Faculty, 20(3): 688-697.
• Zhang, T., Chen, J., Wang, C., Shi, W. and Li, D. (2018). The therapeutic effect of Yinhuangerchen mixture on Avian infectious laryngotracheitis. Poultry Science, 97: 2690-2697.