Kök Hücreleri ve Klinik Uygulamaları

Öz

Kök hücreler, çeşitli hücre tiplerine kemik, kıkırdak, kas, yağ, v.b. dönüşebilme potansiyeline sahip plastisite farklılaşmamış hücrelerdir. Bu hücrelerin plastisiteleri, her gelişim evresinde farklı derecelerde gözlenmektedir. İzole edildikleri gelişim dönemine göre kök hücreler, embriyonik, uyarılmış pluripotent ve yetişkin hücreler olmak üzere üç ana sınıfa ayrılmaktadır. Günümüzde, klinik uygulamalarda oldukça önemli yer tutan kök hücreler mezenkimal kök hücreler, kanser kök hücreleri, v.b. , özellikle yeni teşhis ve tedavi yöntemlerinin geliştirilmesinde umut verici bir hücre kaynağı oluşturmaktadır.

Anahtar Kelimeler

• Embriyonik kök hücreler, uyarılmış pluripotent kök hücreler, yetişkin kök hücreler, kanser kök hücreleri, teşhis, tedavi

STEM CELLS AND CLINICAL APPLICATIONS

Öz

Stem cells are undifferentiated cells which have a differentiation capacity plasticity to the various cell types bone, cartilage, muscle, adipose, etc. . Plasticities of these cells are observed in different degrees for each developmental stage. According to development stage from which these cells are isolated they classify into three main type and they are called embriyonic, induced pluripotent and adult stem cells. Nowadays, stem cells mesencymal stem cells, cancer stem cells, etc. which are very important in clinical applications constitutes a promising cell source especially for developing new diagnosis and treatment techniques.

Anahtar Kelimeler

• Embriyonic stem cells, induced pluripotent stem cells, adult stem cells, cancer stem cells, diagnosis, treatment

Kaynakça

1. [1] Aasen T, Belmonte, JCI [2010]. Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells. Nature Protocols, 5[2]: 3171-382.
2. [2] Akar AR, Arat M, Beksaç M, Can A, Çamurdanoğlu BZ, Çetinkaya DU, Elçin YM, Kansu E, Kırık D, Özçelik T, Özden İ, Şahin G [2009]. Türkiye Bilimler Akademisi Raporları, 20, Ankara, 113s.
3. [3] Barry FP, Murphy JM [2004]. Mesenchymal Stem Cells: Clinical applications and biological characterization. The International Journal of Biochemistry & Cell Biology, 36: 568-584.
4. [4] Deliloğlu Gürhan Sİ, Özen MÖ, Sözer P, Lüleci İ [2009]. Kök hücreler ve doku mühendisliği, Sağlıkta Birikim, 1[5]: 143-168.
5. [5] Ghiaur G, Gerber J, Jones RJ [2012]. Concise review: Cancer stem cells and minimal residual disease. Stem Cells, 30: 89 – 93.
6. [6] Gimble JM, Guilak F [2003]. Adipose-derived adult stem cells: Isolation, characterization and differentiation potential, Cytotherapy, 5[5]: 362-369.
7. [7] Kapinas K, Grandy R, Ghule P, Medina R, Becker K, Pardee A, Zaidi SK, Lian J, Stein J, Wijnen AV, Stein G, [2013]. The abbreviated pluripotent cell cycle, Journal of Cellular Physiology, 228: 9-20.
8. [8] Lanza R, Klimanskaya I [2009]. Essential Stem Cell Methods, Academic Press Elsevier, USA, 608p.

9. [9] Lodish H, Berk A, Matsudaira P, Kaiser CA, Krieger CA, Scott MP, Zipursky SL, Darnell J [2004]. Molecular Cell Biology, W. H. Freeman and Company, New York, 1050p.
10. [10] Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR [1999]. Multilineage potential of adult mesenchymal stem cells, Science, 284: 143-147.
11. [11] Polak JM, Bishop AE [2006]. Stem cells and tissue engineering: past, present, and future, Annals of the New York Academy of Sciences, 1068: 352–366.
12. [12] Reya T, Morrison SJ, Clarke MF, Weismann IL [2001]. Stem cells, cancer and cancer stem cells. Nature, 414: 105 – 111.
13. [13] Sadler TW [2005]. Langman’s Medikal Embriyoloji, [Çev. A. C. Başaklar], Palme Yayınları, 303, Ankara, 507s.
14. [14] Spillane JB, Henderson, MA [2007]. Cancer stem cells: a review. ANZ Journal of Surgery., 77: 464 – 468.
15. [15] Stolpe, A [2013]. On the origin and destination of cancer stem cells: a conceptual evaluation. American Journal of Cancer Research, 3[1]: 107 – 116.
16. [16] Zhu Y, Liu T, Song K, Fan X, Ma X, Cui Z [2008]. Adipose-derived stem cell: a better stem cell than BMSC, Cell Biochemistry and Function, 26: 664-675.
17. [17] Zuk PA, Zhu M, Ashjian P, Daniel AU, Huang JI, Mizuno H, Alfonso Z, Fraser JK, Benhaim P, Hedrick MH [2002]. Human adipose tissue is a source of multipotent stem cells, Molecular Biology of the Cell, 13: 4279-4295