A Statistical Study on Chromosome Abnormalities

Yıl 2022, Sayı: 16, 1 - 9, 30.06.2022

Canan Özdemir Ali Özdemir

Öz

Chromosome aberrations have been used as a measure of reproductive success in plants for many years and have been correlated with morphological and taxonomical changes, fertility-sterility relationships, mutations, and other characteristics. Numerous studies have demonstrated that plant
chromosomes are sensitive indicators to environmental pollutants. In this paper asetic acid organic compound with the chemical formula CH3COOH have been used to illustrate the potential of plant systems as monitors of chromosome aberrations. The many extensively used chemicals are known to induce a wide range of chromosome aberrations. Acetic acid is the second simplest carboxylic acid (after formic acid). It is an important chemical reagent and industrial chemical, used primarily in the production of cellulose acetate for photographic film, polyvinyl acetate for wood glue, and synthetic fibres and fabrics. In households, diluted acetic acid is often used in descaling agents. In the food industry, acetic acid is controlled by the food additive code E260 as an acidity regulator and as a condiment. In biochemistry, the acetyl group, derived from acetic acid, is fundamental to all forms of life.
In this study, the effect of acetic acid, which has entered our lives with different uses, on onion (Allium cepa L.) chromosomes was investigated. In order to obtain root tips, the bulbs of the plant were kept in 5, 25 and 70% solutions of acetic acid prepared with distilled water for 2 and 12 hours. In the chromosomes examined at the root tips obtained as a result of acetic acid treatment; Abnormalities such as bridge formation, adhesion, irregular distribution, fishbone and fan, ring chromosome were observed. The results were evaluated numerically and statistical analyzes were made.

Anahtar Kelimeler

Abnormalities, acid, chromosome, plant, statistical

Kaynakça

• [1] Grant W.F, (1978). Chromosome aberrations in plants as a monitoring system. Environmental Health Perspectives Vol. 27, pp. 37-43.
• [2] Coşkun F. (2006). Gıdalarda Bulunan Doğal Koruyucular. Gıda Teknolojileri Elektronik Dergisi 2: 27-33, 2006.
• [3] Wagner, F S., & Staff, U. (2014). Acetic Acid Kirk-Othmer Encyclopedia of Chemical Technology, 1–21.
• [4] Inceer H, O Beyazoğlu. (2000). Bakır Klorür’ün Vicia hirsuta (L.) S.F. Gray Kök Ucu Hücreleri Üzerine Sitogenetik Etkileri, Turkish Journal of Biology. 24: 553-559.
• [5] Inceer H, Ayaz S, Beyazoğlu O, Enturk E, (2003). Bakır klorür’ün Helianthus annuus (L.) kök ucu hücreleri üzerine sitogenetik etkileri, Turkish Journal of Biology. 27: 43-46.
• [6] Shraideh ZA. (2010).Toxicity of triethyllead chloride (TriEL) on cytoplasmic shuttle streaming, structure, growth and migration of the plasmodial slime mold Physarum polycephalum, Jordan Journal of Biological Sciences, 3: 33 – 40.
• [7] Özdemir A, BozdağB, Sepet H, Parlak Y, Kocabaş O, SerapE, Özdemir C. (2015)Cytogenetic effects of technetium on meristematic cells of root tips of Vicia faba L. and statistical comparison, Caryologia 68: 200-206.
• [8] Şutan, N.A Uţă G., and Bărbuceanu D. (2018). Oxidative stress and cytogenetic effects in root tip cells of Allium cepa L. induced by alcoholic extracts of Leptinotarsa decemlineata (Say).” Caryologia, 1–9.
• [9] Akar H. Osama Elhamalawy and Aida I. El Makawy. (2020) Estimation of Grape Seed Oil Alleviative Role on Cadmium Toxicity in Male Mice, Jordan Journal of Biological Sciences 13: 77 – 83.
• [10] Tengjaroenkul B. and Neeratanaphan L. (2020). Effect of Cadmium Chloride on Chromosomal Abnormalities in the Nile Tilapia Fish In Vivo,” Jordan Journal of Biological Sciences, 13: 127 – 132.
• [11] Sugiyama S. T. Yoshino, H. Kanahara, M. Shichiri, D. Fukushi, and Ohtani T. (2004). Effects of acetic acid treatment on plant chromosome structures analyzed by atomic force microscopy, Analytical Biochemistry 324: 39–44.
• [12] Darlıngton, CD. La Cour F. (1976). The Handling of Chromosomes. Allen and Unwin De Flora S, Bennıcellı C, Bagnasco M. 1994. Genotoxicity of Mercury Compounds. A Review. Mutat Res. 317: 57- 79.
• [13] Utsumi Y, Utsumi C., Tanaka M, Ha, C. V S. Takahashi, A. (2019) Matsui, M.Seki, Acetic Acid Treatment Enhances Drought Avoidance in Cassava (Manihot esculenta Crantz). Frontiers in Plant Science, 10; 33-36.
• [14] Alaca C. Özdemir A. Bozdağ B., Özdemir C. (2020) Cytogenetic effects of C6H4 (CH3)2 (xylene) on meristematic cells of root tips of Vicia faba L. and mathematical analysis, Caryologia 73(1).