Protective Effects of D-Carvone on Ovarian Ischemia-Reperfusion Injury: An Experimental Study

Öz

Aim: The aim of this study was to measure the protective efficacy of D-carvone against ovarian ischemia-reperfusion (OIR) injury by evaluating apoptosis, inflammation, and oxidative stress. Material and Methods: 24 Wistar albino female rats were randomly divided into three groups with eight animals in each group. In the sham group, a lower abdominal incision was made. In the ovarian ischemia-reperfusion (OIR) group, 3 hours of ischemia followed by 3 hours of reperfusion were applied. In the OIR+Dcarvone group, 20 mg/kg D-carvone was administered intraperitoneally for 14 days. On day 15, after 3 hours of ovarian ischemia, 3 hours of reperfusion were performed by administering the last dose of D-carvone just before reperfusion. ELISA and immunohistological analyses were performed on the ovarian tissues. Results: TNF-α, IL-1β, IL-6, malondialdehyde (MDA), myeloperoxidase (MPO), total oxidative level (TOS), oxidative stress index (OSI), and ischemia-modified albumin (IMA) levels, which were higher in the OIR group compared to the sham group, decreased significantly in the OIR+D-Carvone group. IL-10, total antioxidant level (TAS), glutathione (GSH), and catalase (CAT) levels were significantly higher in the D-carvone-treated group compared to the OIR group and lower in the OIR group compared to the sham group. Ischemia-modified albumin levels of the OIR+D-carvone group were significantly lower compared to the OIR group. 8-OHdG and NFκB, which rise with ischemia reperfusion (IR) injury, were reduced considerably in the treatment group. Caspase 3 immunopositivity is significantly lower in the OIR+D-carvone group compared to OIR. Conclusion: In the present study, D-carvone showed anti-inflammatory and antioxidant effects against OIR injury, and protective activity was determined. These results suggested that D-carvone may be a potential agent for OIR injury.

Anahtar Kelimeler

• ovary ischemia reperfusion
• oxidative stress
• inflammation
• apoptosis

Kaynakça

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