Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi

Melissa Tatlıdil Akal; Burak Önal; Deniz Özen; Bülent Demir; Ahmet Gökhan Akkan; Sibel Özyazgan

Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi

Öz

ÖZET Amaç: Tipik anjinaya sahip, invaziv olmayan testlerde iskemi bulgusuna rastlanan ve koroner anjiyografide normal epikardiyal koroner arterlere sahip olan hasta popülasyonu Kardiyak Sendrom X (KSX) olarak tanımlanmaktadır. KSX hastalarında CRP ve diğer inflamatuvar belirteçlerin düzeylerinin artması, hastalığın inflamasyon aracılı bir patogeneze sahip olduğunu gösterir niteliktedir. Sitokrom P450 enzimi olan CYP2J2, koroner arter endoteli, düz kas hücreleri ve kardiyomiyositlerde yüksek düzeylerde eksprese edilen bir hem protein ailesi üyesidir. CYP2J2’nin başlıca görevi, araşidonik asidin; 5,6- 8,9- 11,12- 14,15- olmak üzere 4 farklı çeşit epoksieikozatrienoik asitlere (EET) dönüşümünü sağlamaktır. Bu EET’ler, TNF-α ile uyarılan VCAM-1’in, endotelyal ekspresyonunu azaltarak inflamatuvar yanıt oluşumunu baskılamakta ve NF-κB’yi de inhibe ederek vasküler duvara lökosit adezyonunu azaltmaktadır. CYP2J2 geni -76G>T polimorfizmi, hücre içerisinde CYP2J2 ve metabolitlerinin ekspresyonunda düşüşe neden olmaktadır. Biz bu çalışmamızda, patofizyolojisinde inflamatuvar süreçlerin etkili olduğu düşünülen KSX hastalığı ile inflamatuvar yolakta rol aldığı bilinen CYP2J2 geni -76G>T polimorfizmi arasındaki bağlantıyı incelemeyi amaçladık. Materyal ve Metot: Ardışık 125 KSX hastası ve 125 sağlıklı bireyden oluşan kontrol grubunu, CYP2J2 geni -76G>T polimorfizmi açısından karşılaştırdık. DNA örneklerinden CYP2J2 geni -76G>T polimorfizminin genotiplemesi gerçek zamanlı PCR metodu kullanılarak yapılmıştır. Bulgular: Hasta ve kontrol gruplarında görülen TT, GT ve GG genotipleri incelendiğinde, istatistiksel olarak anlamlı bir fark gözlenmemiştir (p>0,05). Sonuç: Bu durum KSX hastalığının patogenezinde, inflamasyon artışına neden olan CYP2J2 geni -76G>T polimorfizminin önemli bir rolü olmadığını düşündürmektedir.

Anahtar Kelimeler

kardiyak sendrom X; inflamasyon; CYP2J2; polimorfizm; mikrovasküler disfonksiyon

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Melissa Tatlıdil Akal Bu kişi benim
Türkiye

Burak Önal Bu kişi benim
Türkiye

Deniz Özen Bu kişi benim
Türkiye

Bülent Demir Bu kişi benim
Türkiye

Ahmet Gökhan Akkan Bu kişi benim
Türkiye

Sibel Özyazgan Bu kişi benim
Türkiye

Yayımlanma Tarihi

1 Ağustos 2019

Gönderilme Tarihi

7 Mayıs 2019

Kabul Tarihi

19 Temmuz 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 9 Sayı: 2

IZ

https://izlik.org/JA37TF79XP

Kaynak Göster

RIS / Bibtex

APA

Tatlıdil Akal, M., Önal, B., Özen, D., Demir, B., Akkan, A. G., & Özyazgan, S. (2019). Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi. Kafkas Journal of Medical Sciences, 9(2), 79-86. https://izlik.org/JA37TF79XP

AMA

1.Tatlıdil Akal M, Önal B, Özen D, Demir B, Akkan AG, Özyazgan S. Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi. KAFKAS TIP BİL DERG. 2019;9(2):79-86. https://izlik.org/JA37TF79XP

Chicago

Tatlıdil Akal, Melissa, Burak Önal, Deniz Özen, Bülent Demir, Ahmet Gökhan Akkan, ve Sibel Özyazgan. 2019. “Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi”. Kafkas Journal of Medical Sciences 9 (2): 79-86. https://izlik.org/JA37TF79XP.

EndNote

Tatlıdil Akal M, Önal B, Özen D, Demir B, Akkan AG, Özyazgan S (01 Ağustos 2019) Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi. Kafkas Journal of Medical Sciences 9 2 79–86.

IEEE

[1]M. Tatlıdil Akal, B. Önal, D. Özen, B. Demir, A. G. Akkan, ve S. Özyazgan, “Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi”, KAFKAS TIP BİL DERG, c. 9, sy 2, ss. 79–86, Ağu. 2019, [çevrimiçi]. Erişim adresi: https://izlik.org/JA37TF79XP

ISNAD

Tatlıdil Akal, Melissa - Önal, Burak - Özen, Deniz - Demir, Bülent - Akkan, Ahmet Gökhan - Özyazgan, Sibel. “Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi”. Kafkas Journal of Medical Sciences 9/2 (01 Ağustos 2019): 79-86. https://izlik.org/JA37TF79XP.

JAMA

1.Tatlıdil Akal M, Önal B, Özen D, Demir B, Akkan AG, Özyazgan S. Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi. KAFKAS TIP BİL DERG. 2019;9:79–86.

MLA

Tatlıdil Akal, Melissa, vd. “Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi”. Kafkas Journal of Medical Sciences, c. 9, sy 2, Ağustos 2019, ss. 79-86, https://izlik.org/JA37TF79XP.

Vancouver

1.Melissa Tatlıdil Akal, Burak Önal, Deniz Özen, Bülent Demir, Ahmet Gökhan Akkan, Sibel Özyazgan. Kardiyak Sendrom X Hastalarında CYP2J2 Polimorfizminin Belirlenmesi. KAFKAS TIP BİL DERG [Internet]. 01 Ağustos 2019;9(2):79-86. Erişim adresi: https://izlik.org/JA37TF79XP