Nar Kabuğu Ekstresinin Sıçanlarda Paklitakselle İndüklenen Primer Nöron Hasarına Karşı Koruyucu Etkisi

Yıl 2018, Cilt: 8 Sayı: 3, 149 - 157, 01.12.2018

Muhammed Yayla Damla Çetin Çağlar Demirbağ Pınar Aksu Kılıçle

Öz

terapötikler ile tedavisinin tedaviyi desteklediği ve daha güçlü anti
kanser etki oluştuğu gösterilmiştir. Ancak nar kabuğunun kanser kemoterapisine bağlı gelişebilecek nörotoksisiteye karşı nasıl bir etki
göstereceği konusunda bir bilgi bulunmamaktadır. Çalışmamızda
güçlü antioksidan olan nar kabuğunun sıçanlarda paklitaksel ile indüklenen primer nöron hasarındaki etkilerini biyokimyasal ve moleküler analizler ile ortaya koymayı amaçladık.

Materyal ve Metot: Yeni doğan sıçan yavrularından elde edilen primer nöron hücre kültüründe paklitaksel toksisitesi indüklendi. PPE
200, 300 ve 400 mg/ml dozlarında koruyucu etki için paklitakselden
2 saat önce uygulandı.
Bulgular: Hücre canlılığı için yapılan MTT analizinde paklitaksel 24
saat sonunda nöron hasarı oluşturarak hücre canlılığını azaltmıştır.
PPE ise tüm dozlarda anlamlı bir şekilde paklitaksel toksisitesini
önleyerek hücre canlılığını korumuştur. PPE bu etkilerini oksidatif
stresi azaltarak, antioksidan kapasiteyi artırarak ve aynı zamanda
pro-inflamatuar sitokin olan TNF-α eksresyonunu ve apoptotik
proteinler olan caspase 9 ve 3 ekspresyonunu baskılayarak ortaya
koymuştur.
Sonuç: PPE antioksidan, anti-inflamatuar ve anti-apoptotik etkisi
ile paklitaksele bağlı gelişen primer nöron hasarını önlemiştir.

Anahtar Kelimeler

Apoptozis, nar, nöron, oksidatif stres, paklitaksel

Protective Effects of Pomegranate Peel Extract on Paclitaxel Induced Primary Neuron Damage in Rats

Öz

Aim: The combination of chemotherapeutics and pomegranate
peel extract has been shown to have a stronger anti-cancer effect. However, there is no information about how pomegranate
peel extract will affect the neurotoxicity that may develop due to
cancer chemotherapy. In this study, we aimed to the effects of
strong antioxidant pomegranate peel extract on paclitaxel induced
primary neuron damage in newborn rats by biochemical and molecular analysis.
Material and Method: Paclitaxel toxicity was induced in the primary neuron cell culture derived from newborn rats. PPE 200, 300
and 400 mg/ml doses were applied 2 hours before the paclitaxel
for protective effect.
Results: In the MTT analysis, paclitaxel reduced cell viability by
caused neuronal damage at the end of 24 hours. PPE has demonstrated significant protective effect on cell viability by preventing paclitaxel toxicity at all doses. PPE was demonstrated its effects by reducing oxidative stress, increasing antioxidant capacity, and also by
suppressing the TNF-α expression which is pro-inflammatory cytokine, and caspase 9 and 3 expression which are apoptotic proteins.
Conclusion: PPE prevents primary neuron damage caused
paclitaxel by its antioxidant, anti-inflammatory and anti-apoptotic
effect

Anahtar Kelimeler

Apoptosis, pomegranate, neuron, oxidative stress, paclitaxel

Kaynakça

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