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Pioglitazon Sıçanlarda Yüksek Sükrozla Başlatılmış Metabolik Sendromda Böbrekteki Oksidatif Stresi Azaltır

Yıl 2018, Cilt 8, Sayı 3, 165 - 171, 01.12.2018

Öz

Amaç: Metabolik sendrom (MS), kronik böbrek hastalığının ilerlemesinde önemli bir risk faktörüdür. Tiyazolidindionlar (TZDs) muhtemelen MS’de renovasküler koruma sağlar. Bununla birlikte, yüksek sükroz başlangıçlı MS’de pioglitazonun böbreğe ait oksidatif stres üzerine etkisinin açıklanmasıyla ilgili çalışmalar devam etmektedir. Bu çalışmanın amacı pioglitazonun MS’li sıçanların böbreklerindeki oksidatif stres belirleyicileri üzerine etkisinin incelenmesidir.

Materyal ve Metot: Çalışmada erkek Wistar türü sıçanlar (200-250 g ağırlıkta) kullanıldı. Onlar kontrol grup, MS grup (935 mM sükroz içme sularına katılarak) ve pioglitazon uygulanmış MS grup (MSP); MS grubun 18. haftasından başlayarak 2 hafta pioglitazon (30 mg/kg/gün, gavajla) uygulandı. Aspartate amino transferaz (AST), laktat dehidrojenaz (LDH), toplam oksidan durum (TOS), toplam antioksidan durum (TAS) seviyeleri ticari kitler kullanılarak ölçüldü. Tiyobarbitürik asit reaktif maddeler  (TBARS), indirgenmiş glutatyon (GSH), üre, ürik asit and kreatin ölçüldü. Tiyoredoksin 1 (TRX1) düzeyi sitoplazma ve hücre zarında western blot ile ölçüldü. TRX1 aktivitesi sitoplazma ve hücre zarında ticari kit kullanılarak öçüldü.

Bulgular: Kontrol sıçanları ile karşılaştırıldığında, sükroz ile beslenmiş olan sıçanlar abdominal obezite, insulin direnci, hiperinsülinemi, ve hipertrigliseridemi içeren MS’nin bir çok özelliğini göstermiştir. Bunun yanında, MS’de değişmiş olan AST, LDH, TOS, TAS, TBARS, GSH, üre, ürik asit and kreatin, TRX1 aktivitesi ve protein düzeyleri pioglitazon uygulamasıyla kontrol seviyelerine dönmüştür.

Sonuç: Pioglitazon, MS’li sıçanların böbreklerinde artmış olan oksidatif stresi azaltmıştır. 

Kaynakça

  • 1. Masson W, Epstein T, Huerín M, Lobo LM, Molinero G, Angel A et al. Cardiovascular risk stratification in patients with metabolic syndrome Without diabetes or cardiovascular disease: Usefulness of metabolic syndrome severity score. High Blood Press Cardiovasc Prev. 2017; 24(3):297-303.
  • 2. Huh JH, Yadav D, Kim JS, Son JW, Choi E, Kim SH et al. An association of metabolic syndrome and chronic kidney disease from a 10-year prospective cohort study. Metabolism 2017; 67:54–61.
  • 3. Panwar B, Hanks LJ, Tanner RM, Muntner P, Kramer H, McClellan WM et al. Obesity, metabolic health, and the risk of end-stage renal disease. Kidney Int 2015; 87:1216–22.
  • 4. Prasad GVR. Metabolic syndrome and chronic kidney disease: current status and future directions. World J Nephrol 2014; 3:210–9.
  • 5. Kurella M, Lo JC, Chertow GM. Metabolic syndrome andthe risk for chronic kidney disease among nondiabetic adults. J Am Soc Nephrol. 2005; 16:2134–40.
  • 6. Evans RM. The steroid and thyroid hormone receptor superfamily. Science 1988; 240:889-95.
  • 7. Peraldi P, Xu M, Spiegelman BM. Thiazolidinediones block tumor necrosis factor-alpha-induced inhibition of insulin signaling. J Clinl Invest. 1997; 100:1863.
  • 8. Hallakou S, Doare L, Foufelle F, Kergoat M, Guerre-Millo M, Berthault MF et al. Pioglitazone induces in vivo adipocyte differentiation in the obese zucker fa/fa rat. Diabetes 1997; 46:1393-9.
  • 9. Qi HP, Wang Y, Zhang QH, Guo J, Li L, Cao YG et al. Activation of peroxisome proliferator-activated receptor γ (PPARγ) through NF-κB/BRG1 and TGF-β1 pathways attenuates cardiac remodeling in pressure-overloaded rat hearts. Cell Physiol Biochem 2015; 35:899-912.
  • 10. Zou C, Hu H, Xi X, Shi Z, Wang G, Huang X. Pioglitazone protects against renal ischemia-reperfusion injury by enhancing antioxidant capacity. J Surg Res. 2013; 184:1092-5.
  • 11. Keaney Jr JF, Larson MG, Vasan RS, Wilson PW, Lipinska I, Corey D et al. Obesity and systemic oxidative stress: clinical correlates of oxidative stress in the Framingham Study. Arterioscler Thromb Vasc Biol. 2003; 23:434–9.
  • 12. Elks CM, Reed SD, Mariappan N, Shukitt-Hale B, Joseph JA, Ingram DK et al. A blueberry-enriched diet attenuates nephropathy in a rat model of hypertension via reduction in oxidative stress. PLoS One 6 2011; e24028.
  • 13. Ruiz-Ramirez A, Chavez-Salgado M, Peneda-Flores JA, Zapata E, Masso F, El-Hafidi M. High-sucrose diet increases ROS generation, FFA accumulation, UCP2 level, and proton leak in liver mitochondria. Am J Physiol Endocrinol Metab 2011; 301:E1198-207.
  • 14. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985; 28:412-9.
  • 15. Vasques AC, Rosado LE, Cassia GR, Geloneze B: Critical analysis on the use of the homeostasis model assessment (HOMA) indexes in the evaluation of the insulin resistance and the pancreatic beta cells functional capacity. Arq Bras Endocrinol Metabol 2008; 52:32-9.
  • 16. Wasowicz W, Nève J, Peretz A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem 1993; 39:2522-6.
  • 17. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82(1):70–7.
  • 18. Hammes, S. R. The further redefining of steroid-mediated signaling. Proc Natl Acad Sci 2003; 100(5): 21680–700.
  • 19. Agbafor. K. N., Engwa. A. G., Ude. C. M., Obiudu. I. K. and Festus. B. O. Effect of Aqueous Leave Extract of Ageratum Conyzoides on Blood Glucose, Creatinine and Calcium ion Levels in Albino rats. Journal of pharmaceutical Chemical and Biological Sciences 2015; 3(3): 408-15.
  • 20. Barham, T. Enzymatic Colorimetric Determination of Uric Acid. Journal of Clinical Chemistry 1972; 97(2): 142 -4.
  • 21. Chen J, Muntner P, Hamm LL, Jones DW, Batuman V, Fonseca V et al. The metabolic syndrome and chronic kidney disease in U.S. adults. Ann Intern Med 2004; 140:167–74.
  • 22. Soria A, D’Alessandro ME, Lombardo YB. Duration of feeding on a sucrose-rich diet determines metabolic and morphological changes in rat adipocytes. J Appl Physiol 2001;91:2109-16.
  • 23. Adedara IA, Abolaji AO, Odion BE, Okwudi IJ, Omoloja AA, Farombi EO. Impairment of hepatic and renal functions by 2,5-hexanedione is accompanied by oxidative stress in rats. J Toxicol 2014; 2014:239240.
  • 24. Suanarunsawat T, Ayutthaya WD, Songsak T, Thirawarapan S, Poungshompoo S Lipid-lowering and antioxidative activities of aqueous extracts of Ocimum sanctum L. leaves in rats fed with a high-cholesterol diet. Oxid Med Cell Longev. 2011; 2011: 962025.
  • 25. Akila P, Asaikumar L, Vennila L. Chlorogenic acid ameliorates isoproterenol-induced myocardial injury in rats by stabilizing mitochondrial and lysosomal enzymes. Biomed Pharmacother 2017; 85:582-91.
  • 26. Quigg RJ, Cybulsky AV, Jacobs JB, Salant DJ. Anti-Fx1A produces complement-dependent cytotoxicity of glomerular epithelial cells. Kidney international 1988; 34:43-52.
  • 27. Vikramathithan J, Gautami G, Ganesh I, Srikumar K. Differences in Rat Tissue Lactate Dehydrogenase Activity Caused by Giberellic Acid and Homobrassinolide. Türk Biyokimya Dergisi [Turkish Journal of Biochemistry–Turk J Biochem] 2009; 34(2):57–61.
  • 28. Ghiselli A, Serafini M, Natella F, Scaccini C. Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radic Biol Med. 2000; 29:1106-14.
  • 29. Liu J, Yeo HC, Övervik-Douki E, Hagen T, Doniger SJ, Chyu DW et al. Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants. J Appl Physiol. 2000; 89:21-8.
  • 30. Yamawaki H, Haendeler J, Berk BC. Thioredoxin: a key regulator of cardiovascular homeostasis. Circ Res. 2003; 93:1029-33.

Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats

Yıl 2018, Cilt 8, Sayı 3, 165 - 171, 01.12.2018

Öz

Aim: The metabolic syndrome (MS) is an important risk factor for the development of chronic kidney disease. Thiazolidinediones (TZDs) provide renovascular protection, probably in the MS. However, reports about the effect of pioglitazone on renal oxidative stress in high sucrose diet-induced MS remains to be determined. The aim of this study was to assess the effects of pioglitazone on oxidative stress markers in kidney tissues of MS rats.

Material and Method: Male Wistar rats (200-250g in weight) were used in present study. They were divided as control (Con) group, MS group (receiving 935 mM sucrose in drinking water) and pioglitazone treated MS group (MSP) received pioglitazone treatment (30 mg/kg/day, via gavage) for two weeks at the end of the 18th weeks of MS group. Aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total oxidant status (TOS), and total antioxidant status (TAS) levels were measured using commercial kits. Thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), urea, uric acid and creatinine were measured. Thioredoxin 1 (TRX1) level was measured in cytosol and membrane tissues by western blot. TRX1 activity was measured in cytosol and membrane tissues using commercial kit.

Results: Compared with control rats, the sucrose-fed rats exhibited several characteristics of MS, including central obesity, insulin resistance, hyperinsulinemia, and hypertriglyceridemia. Furthermore, changed levels of AST, LDH, TOS, TAS, TBARS, GSH, urea, uric acid, creatinine and TRX1 activity and protein levels in the MS group were reversed to control levels by administration of pioglitazone.

Conclusion: Pioglitazone reduced the elevated oxidative stress in kidney of MS rats.

Kaynakça

  • 1. Masson W, Epstein T, Huerín M, Lobo LM, Molinero G, Angel A et al. Cardiovascular risk stratification in patients with metabolic syndrome Without diabetes or cardiovascular disease: Usefulness of metabolic syndrome severity score. High Blood Press Cardiovasc Prev. 2017; 24(3):297-303.
  • 2. Huh JH, Yadav D, Kim JS, Son JW, Choi E, Kim SH et al. An association of metabolic syndrome and chronic kidney disease from a 10-year prospective cohort study. Metabolism 2017; 67:54–61.
  • 3. Panwar B, Hanks LJ, Tanner RM, Muntner P, Kramer H, McClellan WM et al. Obesity, metabolic health, and the risk of end-stage renal disease. Kidney Int 2015; 87:1216–22.
  • 4. Prasad GVR. Metabolic syndrome and chronic kidney disease: current status and future directions. World J Nephrol 2014; 3:210–9.
  • 5. Kurella M, Lo JC, Chertow GM. Metabolic syndrome andthe risk for chronic kidney disease among nondiabetic adults. J Am Soc Nephrol. 2005; 16:2134–40.
  • 6. Evans RM. The steroid and thyroid hormone receptor superfamily. Science 1988; 240:889-95.
  • 7. Peraldi P, Xu M, Spiegelman BM. Thiazolidinediones block tumor necrosis factor-alpha-induced inhibition of insulin signaling. J Clinl Invest. 1997; 100:1863.
  • 8. Hallakou S, Doare L, Foufelle F, Kergoat M, Guerre-Millo M, Berthault MF et al. Pioglitazone induces in vivo adipocyte differentiation in the obese zucker fa/fa rat. Diabetes 1997; 46:1393-9.
  • 9. Qi HP, Wang Y, Zhang QH, Guo J, Li L, Cao YG et al. Activation of peroxisome proliferator-activated receptor γ (PPARγ) through NF-κB/BRG1 and TGF-β1 pathways attenuates cardiac remodeling in pressure-overloaded rat hearts. Cell Physiol Biochem 2015; 35:899-912.
  • 10. Zou C, Hu H, Xi X, Shi Z, Wang G, Huang X. Pioglitazone protects against renal ischemia-reperfusion injury by enhancing antioxidant capacity. J Surg Res. 2013; 184:1092-5.
  • 11. Keaney Jr JF, Larson MG, Vasan RS, Wilson PW, Lipinska I, Corey D et al. Obesity and systemic oxidative stress: clinical correlates of oxidative stress in the Framingham Study. Arterioscler Thromb Vasc Biol. 2003; 23:434–9.
  • 12. Elks CM, Reed SD, Mariappan N, Shukitt-Hale B, Joseph JA, Ingram DK et al. A blueberry-enriched diet attenuates nephropathy in a rat model of hypertension via reduction in oxidative stress. PLoS One 6 2011; e24028.
  • 13. Ruiz-Ramirez A, Chavez-Salgado M, Peneda-Flores JA, Zapata E, Masso F, El-Hafidi M. High-sucrose diet increases ROS generation, FFA accumulation, UCP2 level, and proton leak in liver mitochondria. Am J Physiol Endocrinol Metab 2011; 301:E1198-207.
  • 14. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985; 28:412-9.
  • 15. Vasques AC, Rosado LE, Cassia GR, Geloneze B: Critical analysis on the use of the homeostasis model assessment (HOMA) indexes in the evaluation of the insulin resistance and the pancreatic beta cells functional capacity. Arq Bras Endocrinol Metabol 2008; 52:32-9.
  • 16. Wasowicz W, Nève J, Peretz A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem 1993; 39:2522-6.
  • 17. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82(1):70–7.
  • 18. Hammes, S. R. The further redefining of steroid-mediated signaling. Proc Natl Acad Sci 2003; 100(5): 21680–700.
  • 19. Agbafor. K. N., Engwa. A. G., Ude. C. M., Obiudu. I. K. and Festus. B. O. Effect of Aqueous Leave Extract of Ageratum Conyzoides on Blood Glucose, Creatinine and Calcium ion Levels in Albino rats. Journal of pharmaceutical Chemical and Biological Sciences 2015; 3(3): 408-15.
  • 20. Barham, T. Enzymatic Colorimetric Determination of Uric Acid. Journal of Clinical Chemistry 1972; 97(2): 142 -4.
  • 21. Chen J, Muntner P, Hamm LL, Jones DW, Batuman V, Fonseca V et al. The metabolic syndrome and chronic kidney disease in U.S. adults. Ann Intern Med 2004; 140:167–74.
  • 22. Soria A, D’Alessandro ME, Lombardo YB. Duration of feeding on a sucrose-rich diet determines metabolic and morphological changes in rat adipocytes. J Appl Physiol 2001;91:2109-16.
  • 23. Adedara IA, Abolaji AO, Odion BE, Okwudi IJ, Omoloja AA, Farombi EO. Impairment of hepatic and renal functions by 2,5-hexanedione is accompanied by oxidative stress in rats. J Toxicol 2014; 2014:239240.
  • 24. Suanarunsawat T, Ayutthaya WD, Songsak T, Thirawarapan S, Poungshompoo S Lipid-lowering and antioxidative activities of aqueous extracts of Ocimum sanctum L. leaves in rats fed with a high-cholesterol diet. Oxid Med Cell Longev. 2011; 2011: 962025.
  • 25. Akila P, Asaikumar L, Vennila L. Chlorogenic acid ameliorates isoproterenol-induced myocardial injury in rats by stabilizing mitochondrial and lysosomal enzymes. Biomed Pharmacother 2017; 85:582-91.
  • 26. Quigg RJ, Cybulsky AV, Jacobs JB, Salant DJ. Anti-Fx1A produces complement-dependent cytotoxicity of glomerular epithelial cells. Kidney international 1988; 34:43-52.
  • 27. Vikramathithan J, Gautami G, Ganesh I, Srikumar K. Differences in Rat Tissue Lactate Dehydrogenase Activity Caused by Giberellic Acid and Homobrassinolide. Türk Biyokimya Dergisi [Turkish Journal of Biochemistry–Turk J Biochem] 2009; 34(2):57–61.
  • 28. Ghiselli A, Serafini M, Natella F, Scaccini C. Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radic Biol Med. 2000; 29:1106-14.
  • 29. Liu J, Yeo HC, Övervik-Douki E, Hagen T, Doniger SJ, Chyu DW et al. Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants. J Appl Physiol. 2000; 89:21-8.
  • 30. Yamawaki H, Haendeler J, Berk BC. Thioredoxin: a key regulator of cardiovascular homeostasis. Circ Res. 2003; 93:1029-33.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Ayça Bilginoğlu KARA>

Yayımlanma Tarihi 1 Aralık 2018
Yayınlandığı Sayı Yıl 2018, Cilt 8, Sayı 3

Kaynak Göster

Bibtex @araştırma makalesi { kaftbd544703, journal = {Kafkas Journal of Medical Sciences}, issn = {2146-2631}, eissn = {2587-053X}, address = {http://194.27.41.48/meddergi/jvi.asp}, publisher = {Kafkas Üniversitesi}, year = {2018}, volume = {8}, number = {3}, pages = {165 - 171}, title = {Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats}, key = {cite}, author = {Kara, Ayça Bilginoğlu} }
APA Kara, A. B. (2018). Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats . Kafkas Journal of Medical Sciences , 8 (3) , 165-171 . Retrieved from https://dergipark.org.tr/tr/pub/kaftbd/issue/44213/544703
MLA Kara, A. B. "Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats" . Kafkas Journal of Medical Sciences 8 (2018 ): 165-171 <https://dergipark.org.tr/tr/pub/kaftbd/issue/44213/544703>
Chicago Kara, A. B. "Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats". Kafkas Journal of Medical Sciences 8 (2018 ): 165-171
RIS TY - JOUR T1 - Pioglitazon Sıçanlarda Yüksek Sükrozla Başlatılmış Metabolik Sendromda Böbrekteki Oksidatif Stresi Azaltır AU - Ayça BilginoğluKara Y1 - 2018 PY - 2018 N1 - DO - T2 - Kafkas Journal of Medical Sciences JF - Journal JO - JOR SP - 165 EP - 171 VL - 8 IS - 3 SN - 2146-2631-2587-053X M3 - UR - Y2 - 2023 ER -
EndNote %0 Kafkas Tıp Bilimleri Dergisi Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats %A Ayça Bilginoğlu Kara %T Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats %D 2018 %J Kafkas Journal of Medical Sciences %P 2146-2631-2587-053X %V 8 %N 3 %R %U
ISNAD Kara, Ayça Bilginoğlu . "Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats". Kafkas Journal of Medical Sciences 8 / 3 (Aralık 2018): 165-171 .
AMA Kara A. B. Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats. KAFKAS TIP BİL DERG. 2018; 8(3): 165-171.
Vancouver Kara A. B. Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats. Kafkas Journal of Medical Sciences. 2018; 8(3): 165-171.
IEEE A. B. Kara , "Pioglitazone Reduces Oxidative Stress in Kidney Against High Sucrose Diet-Induced Metabolic Syndrome in Rats", Kafkas Journal of Medical Sciences, c. 8, sayı. 3, ss. 165-171, Ara. 2018