Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri

Yıl 2021, Cilt: 11 Sayı: 3, 410 - 416, 01.12.2021

Fikret Altındağ

Öz

Amaç: Hepatotoksisite diyabetin en önemli sekonder komplikasyonlarından biridir. Diyabetin neden olduğu karaciğer hasarının ana nedenleri oksidatif stres ve inflamasyondur. Sinapik asidin güçlü bir antioksidan ve antiinflamatuar olduğu öne sürülmüştür. Bu çalışmada, deneysel diyabet modelinde sinapik asidin (SA) hepatoprotektif etkilerini TNF-α, AST, ALT seviyeleri, MT boyama yoğunluğu ve histolojik değişiklikleri değerlendirerek araştırmayı amaçladık.
Materyal ve Metot: Sıçanlar dört gruba ayrıldı (n=7): Sham (S), SA, Diyabetik (D), Diyabetik+Sinapik Asit (D+SA). S grubuna intragastrik (i.g.) yolla serum fizyolojik verildi. SA grubuna 28 gün boyunca i.g. yolla 20 mg/kg/gün SA verildi. D grubuna tek doz 50 mg/kg STZ intraperitoneal (i.p.) enjekte edildi. D+SA grubuna, tek doz 50 mg/kg STZ i.p. yolla enjekte edildi ve 28 gün boyunca 20 mg/kg/gün SA i.g. yolla verildi. Tümor nekroz faktör-alfa (TNF-α) ekspresyonu immünohistokimyasal yöntemle değerlendirildi. Karaciğerde olası fibrozisi değerlendirmek için Masson’s trichrome (MT) boyası ve histolojik inceleme için hematoksilen-eozin (H-E) boyası ile karaciğer boyandı. Ayrıca karaciğer fonksiyon testleri olan serum AST ve ALT seviyeleri ölçüldü.
Bulgular: S ve SA grupları normal histolojik mimariye ve negatif TNF-a immüno-ekspresyonuna sahipti. S grubu ile karşılaştırıldığında, D grubu daha yüksek AST ve ALT seviyelerine ve MT boyama
yoğunluğuna sahipti. D grubunda şiddetli TNF-α immün ekpresyonunun yanı sıra vasküler dilatasyon, apoptotik hücreler ve inflamatuar hücrelerin infiltrasyonu gibi histopatolojik değişiklikler
gözlendi. D grubu ile karşılaştırıldığında D+SA grubunda TNF-α immunoexpression, histopatolojik değişiklikler, AST ve ALT seviyeleri azaldı.
Sonuç: Çalışmamız, STZ ile indüklenen diyabetik sıçanlarda SA’nın hepatotoksisiteye karşı hepatoprotektif bir etkiye sahip olabileceğini ortaya koydu.

Anahtar Kelimeler

diyabet, karaciğer, sıçan, sinapik asit, TNF-α

Kaynakça

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