The Effects of Engeletin on Cell Proliferation and Invasion in the Human Breast Cancer Cell Line (MCF-7)

Year 2022, Volume: 12 Issue: 2, 135 - 140, 31.08.2022

Erdem Toktay

Abstract

Aim: Belonging to the group of flovonoids, Engeletin is a molecule with strong anti-inflammatory, antioxidant and anticancer properties. However, the effect of this molecule on breast cancer cells has not been studied yet. For this purpose, the effectiveness of Engeletin (ENG) on cell proliferation, invasion and apoptosis in the human breast cancer cell line (MCF-7) was investigated in this study,
Materials and Methods: ENG was studied at 1, 10 and 100 µM doses in the MCF-7 cell line. In the study, cell proliferation was analyzed by MTT cell viability test, its effectiveness on invasion was analyzed by transwell assay, cellular viability and apoptotic evaluation were analyzed by fluorescence staining method.
Results: It was determined that Engeletin reduced MCF-7 cell proliferation. The ENG 100 µM dose was found to be the most effective dose. While ENG application decreases the number of viable cells, it causes an increase in the number of apoptotic cells. In addition, it was determined that ENG application significantly decreased the number of invasive cells in a dose-dependent manner compared to the control group (p<0.001).
Conclusion: Engeletin is a molecule with anti-carcinogenic, antiproliferative activity on MCF-7 cells. In addition, ENG shows an anti-invasive activity in MCF-7 cells, demonstrating that it is a molecule with anti-metastatic activity.

Keywords

Engeletin, Cell Proliferation, Cell Viability, Invasion and MCF-7

References

• 1. Wang, R.C., X. Chen, A.M. Parissenti, A.A. Joy, J. Tuszynski, D.N. Brindley et al. Sensitivity of docetaxel-resistant MCF-7 breast cancer cells to microtubule-destabilizing agents including vinca alkaloids and colchicine-site binding agents. PLoS One 2017; 12(8): e0182400.
• 2. Lal, S., A. Mahajan, W.N. Chen and B. Chowbay Pharmacogenetics of target genes across doxorubicin disposition pathway: a review. Curr Drug Metab 2010; 11(1): 115-28.
• 3. Redig, A.J. and S.S. McAllister Breast cancer as a systemic disease: a view of metastasis. J Intern Med 2013; 274(2): 113-26.
• 4. Shareef, M., M.A. Ashraf and M. Sarfraz Natural cures for breast cancer treatment. Saudi Pharm J 2016; 24(3): 233-40.
• 5. Levitsky, D.O. and V.M. Dembitsky Anti-breast Cancer Agents Derived from Plants. Nat Prod Bioprospect 2014.
• 6. Christaki, E., E. Bonos, I. Giannenas and P. Florou-Paneri Aromatic plants as a source of bioactive compounds. Agriculture 2012; 2(3): 228-243.
• 7. Yao, L.H., Y.M. Jiang, J. Shi, F.A. Tomas-Barberan, N. Datta, R. Singanusong et al. Flavonoids in food and their health benefits. Plant Foods Hum Nutr 2004; 59(3): 113-22.
• 8. Surh, Y.J. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer 2003; 3(10): 768-80.
• 9. Romagnolo, D.F. and O.I. Selmin Flavonoids and cancer prevention: a review of the evidence. J Nutr Gerontol Geriatr 2012; 31(3): 206-38.
• 10. Kopustinskiene, D.M., V. Jakstas, A. Savickas and J. Bernatoniene Flavonoids as Anticancer Agents. Nutrients 2020; 12(2).
• 11. Lee, H.H., J. Jung, A. Moon, H. Kang and H. Cho Antitumor and Anti-Invasive Effect of Apigenin on Human Breast Carcinoma through Suppression of IL-6 Expression. Int J Mol Sci 2019; 20(13).
• 12. Liskova, A., L. Koklesova, M. Samec, K. Smejkal, S.M. Samuel, E. Varghese et al. Flavonoids in Cancer Metastasis. Cancers (Basel) 2020; 12(6).
• 13. Tian, Q., G. Wang, Y. Zhang, F. Zhang, L. Yang, Z. Liu et al. Engeletin inhibits Lipopolysaccharide/d-galactosamine-induced liver injury in mice through activating PPAR-gamma. J Pharmacol Sci 2019; 140(3): 218-222.
• 14. Jiang, X., L. Chen, Z. Zhang, Y. Sun, X. Wang and J. Wei Protective and Therapeutic Effects of Engeletin on LPS-Induced Acute Lung Injury. Inflammation 2018; 41(4): 1259-1265.
• 15. Huang, Z., H. Ji, J. Shi, X. Zhu and Z. Zhi Engeletin Attenuates Abeta1-42-Induced Oxidative Stress and Neuroinflammation by Keap1/Nrf2 Pathway. Inflammation 2020; 43(5): 1759-1771.
• 16. Bai, H. and H. Yin Engeletin suppresses cervical carcinogenesis in vitro and in vivo by reducing NF-kappaB-dependent signaling. Biochem Biophys Res Commun 2020; 526(2): 497-504.
• 17. Huber, M.A., N. Azoitei, B. Baumann, S. Grunert, A. Sommer, H. Pehamberger et al. NF-kappaB is essential for epithelial-mesenchymal transition and metastasis in a model of breast cancer progression. J Clin Invest 2004; 114(4): 569-81.
• 18. Yayla, M., D. Çetin, Ç. Demirbağ and P.A. KILIÇLE Nar Kabuğu Ekstresinin Sıçanlarda Paklitakselle İndüklenen Primer Nöron Hasarına Karşı Koruyucu Etkisi. Kafkas Journal of Medical Sciences 2018; 8(3): 149-157.
• 19. Yayla, M., Ü. Harun and D. Binnetoğlu Neuroprotective effects of phloretin and phloridzin on paclitaxel-induced neuronal damage in primary neuron cells. Cukurova Medical Journal 2021; 46(2): 632-639.
• 20. Harun, Ü. and R.A. Ugan Olivetol’ün SHSY-5Y Nöroblastoma Hücrelerinin Proliferasyonu ve İnvazyonu Üzerindeki İnhibe Edici Etkileri. Kafkas Tıp Bilimleri Dergisi 2021; 11(1): 57-62.
• 21. Jemal, A., F. Bray, M.M. Center, J. Ferlay, E. Ward and D. Forman Global cancer statistics. CA Cancer J Clin 2011; 61(2): 69-90.
• 22. Weigelt, B., J.L. Peterse and L.J. van 't Veer Breast cancer metastasis: markers and models. Nat Rev Cancer 2005; 5(8): 591-602.
• 23. Mehta, R.G., G. Murillo, R. Naithani and X. Peng Cancer chemoprevention by natural products: how far have we come? Pharm Res 2010; 27(6): 950-61.
• 24. Ma, L., M. Zhang, R. Zhao, D. Wang, Y. Ma and A. Li Plant Natural Products: Promising Resources for Cancer Chemoprevention. Molecules 2021; 26(4).
• 25. Ravishankar, D., A.K. Rajora, F. Greco and H.M. Osborn Flavonoids as prospective compounds for anti-cancer therapy. Int J Biochem Cell Biol 2013; 45(12): 2821-31.
• 26. Liu, T., Y. Li, J. Sun, G. Tian and Z. Shi Engeletin suppresses lung cancer progression by inducing apoptotic cell death through modulating the XIAP signaling pathway: A molecular mechanism involving ER stress. Biomed Pharmacother 2020; 128110221.
• 27. Wungsintaweekul, B., K. Umehara, T. Miyase and H. Noguchi Estrogenic and anti-estrogenic compounds from the Thai medicinal plant, Smilax corbularia (Smilacaceae). Phytochemistry 2011; 72(6): 495-502.
• 28. Briand, P. and A.E. Lykkesfeldt Effect of estrogen and antiestrogen on the human breast cancer cell line MCF-7 adapted to growth at low serum concentration. Cancer Res 1984; 44(3): 1114-9.
• 29. Boyd, V., O.M. Cholewa and K.K. Papas Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell Permeable Nucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm 2008; 2(2): 66-84.
• 30. Lecoeur, H. Nuclear apoptosis detection by flow cytometry: influence of endogenous endonucleases. Exp Cell Res 2002; 277(1): 1-14.
• 31. Suzuki, T., K. Fujikura, T. Higashiyama and K. Takata DNA staining for fluorescence and laser confocal microscopy. J Histochem Cytochem 1997; 45(1): 49-53.
• 32. Dou, Q.Q., C.P. Teng, E. Ye and X.J. Loh Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers. Int J Nanomedicine 2015; 10419-32.
• 33. Justus, C.R., N. Leffler, M. Ruiz-Echevarria and L.V. Yang In vitro cell migration and invasion assays. J Vis Exp 2014(88).
• 34. Li, S., Y. Wang, C. Feng, G. Wu, Y. Ye and J. Tian Calycosin Inhibits the Migration and Invasion of Human Breast Cancer Cells by Down-Regulation of Foxp3 Expression. Cell Physiol Biochem 2017; 44(5): 1775-1784.
• 35. Wu, H., G. Zhao, K. Jiang, C. Li, C. Qiu and G. Deng Engeletin Alleviates Lipopolysaccharide-Induced Endometritis in Mice by Inhibiting TLR4-mediated NF-kappaB Activation. J Agric Food Chem 2016; 64(31): 6171-8.