Determination of Microbial Flora of Model Insect Galleria mellonella in Different Life Forms Reared on Artificial Diet

Öz

Model insects are used as an alternative experimental model to vertebrate experimental animals in many fields such as medicine, pharmacy and veterinary medicine due to their low production cost, ethical problems and easy culture in laboratory conditions. The most common problem encountered during the production of model insects in artificial diet under laboratory conditions is bacteria, yeast and mold contamination. In this study, seven different bacterial species, four gram negative and three gram positive, were identified in vitro in different life stages of the model insect Galleria mellonella. As microfungus genera Alternaria spp., Aspergillus spp., Beauveria sp., Penicillium spp. and Verticillium sp. The highest number of colonies in their life stages is defined as the species belonging to the genus Aspergillus and Penicillium, while the least belong to the genus Verticillium determined. This study is very important in terms of defining microflora in artificial diet and standardizing production areas of insects under laboratory conditions

Anahtar Kelimeler

• Galleria mellonella
• Microflora
• Microfungus
• Model insect
• Artificial diet

Yapay Besin Ortamında Yetiştirilen Model Böcek Galleria mellonella’nın Farklı Yaşam Evrelerindeki Mikrobiyal Floranın Belirlenmesi

Öz

Model böcekler düşük üretim maliyeti, etik sorunlar ve laboratuvar koşullarında kolay kültüre alınabilmesi nedeniyle tıp, eczacılık ve veterinerlik gibi birçok alanda omurgalı deney hayvanlarına alternatif deneysel model olarak kullanılmaktadır. Model böceklerin laboratuvar koşullarında yapay besin ortamında üretilmesi sırasında en çok karşılaşılan sorun bakteri, maya ve küf kontaminasyonudur. Bu çalışmada model böcek Galleria mellonella’nın farklı yaşam evrelerinde dört gram negatif ve üç gram pozitif olmak üzere yedi farklı bakteri türü in vitro olarak tanımlandı. Mikrofungus cinsleri; Alternaria spp., Aspergillus spp., Beauveria sp., Penicillium spp. ve Verticillium sp. olarak belirlendi. Yaşam evrelerinde en fazla koloni sayısı Aspergillus ve Penicillium cinsine ait türlerde tanımlanırken, en az koloni sayısının ise Verticillium cinsine ait olduğu belirlendi. Bu çalışma yapay besin ortamında mikrofloranın tanımlanması ve laboratuvar koşullarında böcek üretim alanlarının standardize edilmesi açısından oldukça önemlidir.

Anahtar Kelimeler

• Model böcek
• mikrofungus
• yapay besin
• mikroflora
• Galleria mellonella

Kaynakça

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