Nano Ozon Yağının B-16 Melanoma ve OV-90 Over Hücrelerinde ROS Üretimi ve ɣH2AX Hücre Pozitifliğini Tetiklemesi

Öz

Tumör hipoksisi, kanser tedavisinde tedaviyi kısıtlayan bir faktördür. Ozon terapi, tumor hipoksisini azaltmak ya da önlemek amaçlı kullanılır. Bu çalışmada, kanser tedavisinde ozon terapinin kısıtlamalarının üstesinden gelmek için, ozon yağı nanoemülsiyonu (OZNE) kullanımı amaçlanmaktadır. OZNE’nin kanser hücre hatlarına olan etkisi, Reaktif Oksijen Türevleri (ROS) ve ɣH2AX testleri ile değerlendirilmiştir. B-16 melanoma ve OV-90 over hücre hatlarının her ikisinde de, ROS üretimi OZNE doz artışı ile artmıştır (B-16 melanoma için %0.85’den %14.88’e, OV-90 over için %1.94’den %58.58’e), OV-90 over hücreleri ɣH2AX hücre pozitifliği B-16 melanom ile kıyaslandığında 5 kat fazla artış göstermiştir. OZNE tedavisi gelecekte kanser hücrelerine hasar verecek yeni etkili bir yöntem sağlayabilir.

Anahtar Kelimeler

• Ozon yağı
• nano
• ɣH2AX
• ROS
• B-16 melanoma
• OV-90 over

Proje Numarası

2016-39971044-02

Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells

Öz

Tumor hypoxia is a restrictive factor for cancer treatment. Ozone therapy, used to decrease or prevent the hypoxia in tumor. In this study, it is aimed to use ozonized oil nanoemulsions (OZNEs) to overcome the limitations of ozone therapy in cancer research. The influence of OZNE against cancer cell lines is evaluated by Reactive Oxygen Species (ROS) and ɣH2AX assays. For both B-16 melanoma and OV-90 cell lines, ROS production levels increased due to the increase in OZNE doses (0.85% to 14.88% and 1.94% to 58.58% for B-16 and OV-90 cell lines, respectively). 5-fold higher ɣH2AX cell positivity’s determined in OV-90 cells compared to B-16 melanoma cells. OZNE treatment could provide a new effective method to damage cancer cells in the future.

Anahtar Kelimeler

• Ozonized oil
• nano
• ɣH2AX
• ROS
• B-16 melanoma
• OV-90 ovarian

Destekleyen Kurum

Bülent Ecevit University Scientific Research Projects

Proje Numarası

2016-39971044-02

Kaynakça

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