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Effects of Oxfendazole on Metabolic Enzymes in Hemolymph of Galleria mellonella L. Lepidoptera: Pyralidae Larvae Reared on Artificial Diet

Yıl 2018, Cilt 8, Sayı 2, 590 - 594, 01.06.2018

Öz

Oxfendazole is used as an antihelminthic drug in parasite infections of animals. Oxfendazole was added at different concentrations 0, 0.0015, 0.015, 0.15, and 1.5% to artificial diet and the wax moth Galleria mellonella larvae were reared to 7th stage. The changes in metabolic enzymes activity in G. mellonella hemolymph tissue were investigated. It was found that the activities of alanine aminotransferase ALT and aspartate aminotransferase AST was increased which are cell damage biomarkers. The highest concentration of this antihelmintic was increased the enzme activity ALT from 329 ± 4.33 to 529. 5 ± 3.17 U/L. Gamma glutamyl transferase GGT enzyme activity was increased from 0.87 ± 0.1 to 4.32 ± 0.22 U/L. Creatine kinase CK and amylase AMYL activities were significantly increased in hemolymph of G. mellonella larvae fed with the highest oxfendazole concentration 1.5% when compared to the control group. Our results indicate that oxfendazole can be used as an insecticide with a well-adjusted concentrations because of its low acute toxicity to humans and other non-target organisms.

Kaynakça

  • Bronskill, J. 1961. A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae). J. Lep. Soc. 15: 102-104.
  • Büyükgüzel, E., Büyükgüzel, K., Adamski, Z., Marciniak, P., Ventrella, E., Bufo, A., Erdem, M., Ziemnicki, K. 2013. The influence of diatery α-solanine on the waxmoth Galleria mellonella. Arch. Insect. Biochem. Physiol. 0:1-10
  • Büyükgüzel, E., Hyršl, P., Büyükgüzel, K. 2010. Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comp. Biochem. Physiol. 156: 176-183.
  • Büyükgüzel, E., Kalender, Y. 2007. Penicillin-induced oxidative stress: Effects on antioxidative response of midgut tissues in larval instars of Galleria mellonella. J. Econ. Entomol. 100: 1533- 1541.
  • Büyükgüzel, E., Kalender, Y. 2009. Exposure to streptomycin alters oxidative and antioxidative response in larval midgut tissues of Galleria mellonella. Pestic. Biochem. Physiol. 94: 112- 118.
  • Büyükgüzel, K. 2001. DNA gyrase inhibitors: Novobiocin enhances the survival of Pimpla turionellae larvae reared on an artificial diet but other antibiotics do not. J. Appl. Entomol. 125: 583-587.
  • Büyükgüzel, K. 2006. Malathion-Induced Oxidative Stress in a Parasitoid Wasp: Effect on Adult Emergence, Longevity, Fecundity, and Oxidative and Antioxidative Response of the Pimpla turionellae (Hymenoptera: Ichneumonidae). J. Econ. Entomol. 99 (4): 1225-1234.
  • Damien, C., Chantal, V. H., Pirouz, S., Zerimech, F. H., Laurence, J., Jean, M. H. 2004. Cellular impact of metal trace elements in terricolous lichen Diploschistes muscorum (Scop.) R. Sant. –identification of oxidative stress biomarkers. Water Air Soil Pollut. 152:55– 69.
  • Eid, MAA., El-Nakkadı, AN., Saleh, MA 1989. Functional adaptation of silk gland after administration of antibiotic to larvae of Philosamaia ricini (Boisd), Insect Sci. Appl. 10: 139- 143.
  • Gaudêncio, FN., Tunholi-Alves, VM., Lima, MG., Gôlo, PS., Angelo, I., Castro, RN., Fonseca, AH., Scott, FB., Pinheiro, J. 2016. Alterations in the Oxidative Metabolism of Rhipicephalus (Boophilus) microplus Ticks in Response to Exposure to the Insect Growth Regulator Fluazuron. Rev. Bras. Parasitol. Vet. 25 (1): 54-60.
  • İçen, E., Armutçu, F., Büyükgüzel, K., Gürel, A. 2005. Biochemical stress indicators of greater wax moth Galleria mellonella L. exposure to organophosphorus insecticides, J. Econ. Entomol. 98: 358-366.
  • Lacey, E., Brady, RL., Prichard, RK., Watson, TR. 1987. Comparison of inhibition of polymerisation of mammalian tubulin and helminth ovicidal activity by benzimidazole carbamates. Vet. Parasitol. 23, 105-119.
  • Maguire, R., Duggan, O., Kavanagh, K. 2016. Evaluation of Galleria mellonella larvae as an in vivo model for assessing the relative toxicity of food preservative agents. Cell Biol Toxicol. 32(3):209-216
  • Mirhaghparast, SK., Zibaee, A., Sendi, JJ., Hoda, H., Dina, MH. 2015. Immune and metabolic responses of Chilo suppressalis Walker (Lepidoptera: Crambidae) larvae to an insect growth regulator, hexaflumuron, Pestic. Biochem. Physiol. 125:69-77.
  • Ramarao,,N., Nielsen-Leroux, C., Lereclus, D. 2012. The Insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis. J. Vis. Exp. 70: 4392.
  • Ramzia, S., Serajia, A., Gonbada AS., Mirhaghparastb, SK., Haghghadamc, ZM., and Haghighata, S. 2017. Toxicity of Artemisia annua (Asteraceae) essential oil on the tea mealy bug, Pseudococcus viburni Sigornet (Hemiptera: Pseudococcidae), Arch Phytopathology Plant Protect. 50:941-956.
  • Rasheed, OF., Al-Shamma, GA., Zillo, BH. 2010. Serum γ-glutamyltransferase as Oxidative Stress Marker in Pre-and Postmenopausal Iraqi Women. Oman. Med. J. 25:286-288.
  • Rosenthal, P. 1997. Assessing liver function and hyperbilirubinemia in the newborn, Clin. Chem, 43: 228-234.
  • Sertçelik, M., Sugeçti, S., Büyükgüzel, E., Necefoğlu, H., Büyükgüzel, K. 2018. Toxicological and Physiological Effects of Diaquabis(N,N-diethylnicotinamide-x N1) bis(4-formylbenzoato- x O)cobalt(II) complex on Galleria mellonella L. (Lepidoptera: Pyralidae) as a model organism. Karaelmas Fen ve Müh. Derg. 359-364.
  • Sidlova, K., Skalıcka, V., Kotaska, K., Pechova, M., Chada, M., Bartosova, J., Hrial, Z., Nevoral, J., Vavrova, V., Prusa, R. 2003. Glutathione S-transferase as a sensitive marker of hepatocellular serum damage in patients with cystic fibrosis, Physiol. Res. 52: 361-365.
  • SPSS Inc. 1997. User’s manual, version 10. SPSS Inc. Chicago, IL.
  • Sugeçti, S., Büyükgüzel, E., Büyükgüzel, K. 2016. Laboratory Assays of the Effects of Oxfendazole on Biological Parameters of Galleria mellonella (Lepidoptera: Pyralidae). J. Entomol. Sci. 51 (2): 129-137.
  • Sugeçti, S., Çelik, C., Büyükgüzel, E., Büyükgüzel, K. 2017. Biochemical Damage and Immuno-physiological Adaptation on Model Organism Galleria mellonella L. (Lepidoptera: Pyralidae) Larvae Exposed to Clinical Pathogen, Klebsiella oxytoca, XIII. Uluslararası Katılımlı Ekoloji ve Çevre Kongresi, s 483, Edirne.
  • Timmermann, SE., Zangerl, AR., Berenbaum, MR. 1999. Ascorbic and uric acid responses to xanthotoxin ingestion in a generalis and specialist caterpillar. Arch Insect Biochem Physiol. 42, 26-36.
  • Uda, K., Fujimoto, N., Akiyama, Y., Mizuta, K., Tanaka, K., Ellington ,WR., Suzuki, T. 2006. Evolution of the arginine kinase gene family. Comp. Biochem. Physiol. D Genomics Proteomics. 1(2):209–218.
  • Verma, KVS., Rahman, SJ. 1984. Comparative efficacy of synthetic pyrethroids, natural pyrethroids and DDT against mosquito larvae, J. Commun. Dis. 16: 144-147.
  • Whitfield, JB. 2001. Gamma glutamyl transferase. Crit. Rev. Clin. Lab. Sci.38:263-355.

Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi

Yıl 2018, Cilt 8, Sayı 2, 590 - 594, 01.06.2018

Öz

Oksfendazol, hayvanlarda görülen pazariter enfeksiyonlarda kullanılan antihelmintik bir ilaçtır. Yapay besine farklı konsantrasyonlarda % 0, 0,0015, 0,015, 0,15, ve 1,5 oksfendazol ilave edilerek, büyük bal mumu güvesi Galleria mellonella larvaları 7. evreye kadar beslendi. G mellonella hemolenf dokusundaki metabolik enzim aktivitesindeki değişimler araştırıldı. Hücre hasarı biyobelirteci alanin aminotransferaz ALT ve aspartat aminotransferaz AST aktivitelerinin arttığı belirlendi. Antihelmintik maddenin en yüksek konsantrasyonda enzim aktivitesi 329 ± 4,33’den 529, 5 ± 3,17 U/L’ye yükseldi. Gama glutamil transferaz GGT enzim aktivitesi 0,87 ± 0,1’den 4,32 ± 0,22 U/L’ ye yükseldi. Kreatin kinaz CK ve amilaz AMLY enzim aktivitelerinin, en yüksek oksfendazol konsantrasyonu % 1,5 ile beslenen G. mellonella larvalarının hemolenf doksunda kontrol grubu ile karşılaştırıldığında istatistiksel olarak önemli derecede arttığı tespit edildi. Bu çalışma; oksfendazolün insan ve diğer hedef olmayan organizmalara karşı düşük akut toksisiteye sahip olması sebebiyle konsantrasyonlarının iyi ayarlanması ile insektisit olarak kullanılabileceğini işaret etmektedir

Kaynakça

  • Bronskill, J. 1961. A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae). J. Lep. Soc. 15: 102-104.
  • Büyükgüzel, E., Büyükgüzel, K., Adamski, Z., Marciniak, P., Ventrella, E., Bufo, A., Erdem, M., Ziemnicki, K. 2013. The influence of diatery α-solanine on the waxmoth Galleria mellonella. Arch. Insect. Biochem. Physiol. 0:1-10
  • Büyükgüzel, E., Hyršl, P., Büyükgüzel, K. 2010. Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comp. Biochem. Physiol. 156: 176-183.
  • Büyükgüzel, E., Kalender, Y. 2007. Penicillin-induced oxidative stress: Effects on antioxidative response of midgut tissues in larval instars of Galleria mellonella. J. Econ. Entomol. 100: 1533- 1541.
  • Büyükgüzel, E., Kalender, Y. 2009. Exposure to streptomycin alters oxidative and antioxidative response in larval midgut tissues of Galleria mellonella. Pestic. Biochem. Physiol. 94: 112- 118.
  • Büyükgüzel, K. 2001. DNA gyrase inhibitors: Novobiocin enhances the survival of Pimpla turionellae larvae reared on an artificial diet but other antibiotics do not. J. Appl. Entomol. 125: 583-587.
  • Büyükgüzel, K. 2006. Malathion-Induced Oxidative Stress in a Parasitoid Wasp: Effect on Adult Emergence, Longevity, Fecundity, and Oxidative and Antioxidative Response of the Pimpla turionellae (Hymenoptera: Ichneumonidae). J. Econ. Entomol. 99 (4): 1225-1234.
  • Damien, C., Chantal, V. H., Pirouz, S., Zerimech, F. H., Laurence, J., Jean, M. H. 2004. Cellular impact of metal trace elements in terricolous lichen Diploschistes muscorum (Scop.) R. Sant. –identification of oxidative stress biomarkers. Water Air Soil Pollut. 152:55– 69.
  • Eid, MAA., El-Nakkadı, AN., Saleh, MA 1989. Functional adaptation of silk gland after administration of antibiotic to larvae of Philosamaia ricini (Boisd), Insect Sci. Appl. 10: 139- 143.
  • Gaudêncio, FN., Tunholi-Alves, VM., Lima, MG., Gôlo, PS., Angelo, I., Castro, RN., Fonseca, AH., Scott, FB., Pinheiro, J. 2016. Alterations in the Oxidative Metabolism of Rhipicephalus (Boophilus) microplus Ticks in Response to Exposure to the Insect Growth Regulator Fluazuron. Rev. Bras. Parasitol. Vet. 25 (1): 54-60.
  • İçen, E., Armutçu, F., Büyükgüzel, K., Gürel, A. 2005. Biochemical stress indicators of greater wax moth Galleria mellonella L. exposure to organophosphorus insecticides, J. Econ. Entomol. 98: 358-366.
  • Lacey, E., Brady, RL., Prichard, RK., Watson, TR. 1987. Comparison of inhibition of polymerisation of mammalian tubulin and helminth ovicidal activity by benzimidazole carbamates. Vet. Parasitol. 23, 105-119.
  • Maguire, R., Duggan, O., Kavanagh, K. 2016. Evaluation of Galleria mellonella larvae as an in vivo model for assessing the relative toxicity of food preservative agents. Cell Biol Toxicol. 32(3):209-216
  • Mirhaghparast, SK., Zibaee, A., Sendi, JJ., Hoda, H., Dina, MH. 2015. Immune and metabolic responses of Chilo suppressalis Walker (Lepidoptera: Crambidae) larvae to an insect growth regulator, hexaflumuron, Pestic. Biochem. Physiol. 125:69-77.
  • Ramarao,,N., Nielsen-Leroux, C., Lereclus, D. 2012. The Insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis. J. Vis. Exp. 70: 4392.
  • Ramzia, S., Serajia, A., Gonbada AS., Mirhaghparastb, SK., Haghghadamc, ZM., and Haghighata, S. 2017. Toxicity of Artemisia annua (Asteraceae) essential oil on the tea mealy bug, Pseudococcus viburni Sigornet (Hemiptera: Pseudococcidae), Arch Phytopathology Plant Protect. 50:941-956.
  • Rasheed, OF., Al-Shamma, GA., Zillo, BH. 2010. Serum γ-glutamyltransferase as Oxidative Stress Marker in Pre-and Postmenopausal Iraqi Women. Oman. Med. J. 25:286-288.
  • Rosenthal, P. 1997. Assessing liver function and hyperbilirubinemia in the newborn, Clin. Chem, 43: 228-234.
  • Sertçelik, M., Sugeçti, S., Büyükgüzel, E., Necefoğlu, H., Büyükgüzel, K. 2018. Toxicological and Physiological Effects of Diaquabis(N,N-diethylnicotinamide-x N1) bis(4-formylbenzoato- x O)cobalt(II) complex on Galleria mellonella L. (Lepidoptera: Pyralidae) as a model organism. Karaelmas Fen ve Müh. Derg. 359-364.
  • Sidlova, K., Skalıcka, V., Kotaska, K., Pechova, M., Chada, M., Bartosova, J., Hrial, Z., Nevoral, J., Vavrova, V., Prusa, R. 2003. Glutathione S-transferase as a sensitive marker of hepatocellular serum damage in patients with cystic fibrosis, Physiol. Res. 52: 361-365.
  • SPSS Inc. 1997. User’s manual, version 10. SPSS Inc. Chicago, IL.
  • Sugeçti, S., Büyükgüzel, E., Büyükgüzel, K. 2016. Laboratory Assays of the Effects of Oxfendazole on Biological Parameters of Galleria mellonella (Lepidoptera: Pyralidae). J. Entomol. Sci. 51 (2): 129-137.
  • Sugeçti, S., Çelik, C., Büyükgüzel, E., Büyükgüzel, K. 2017. Biochemical Damage and Immuno-physiological Adaptation on Model Organism Galleria mellonella L. (Lepidoptera: Pyralidae) Larvae Exposed to Clinical Pathogen, Klebsiella oxytoca, XIII. Uluslararası Katılımlı Ekoloji ve Çevre Kongresi, s 483, Edirne.
  • Timmermann, SE., Zangerl, AR., Berenbaum, MR. 1999. Ascorbic and uric acid responses to xanthotoxin ingestion in a generalis and specialist caterpillar. Arch Insect Biochem Physiol. 42, 26-36.
  • Uda, K., Fujimoto, N., Akiyama, Y., Mizuta, K., Tanaka, K., Ellington ,WR., Suzuki, T. 2006. Evolution of the arginine kinase gene family. Comp. Biochem. Physiol. D Genomics Proteomics. 1(2):209–218.
  • Verma, KVS., Rahman, SJ. 1984. Comparative efficacy of synthetic pyrethroids, natural pyrethroids and DDT against mosquito larvae, J. Commun. Dis. 16: 144-147.
  • Whitfield, JB. 2001. Gamma glutamyl transferase. Crit. Rev. Clin. Lab. Sci.38:263-355.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Research Article
Yazarlar

Serkan SUGEÇTİ
Zonguldak Bulent Ecevit University, Faculty of Science and Arts, Department of Biology, Zonguldak, Turkey
0000-0003-3412-2367


Kemal BÜYÜKGÜZEL Bu kişi benim
Zonguldak Bulent Ecevit University, Faculty of Science and Arts, Department of Biology, Zonguldak, Turkey

Yayımlanma Tarihi 1 Haziran 2018
Yayınlandığı Sayı Yıl 2018, Cilt 8, Sayı 2

Kaynak Göster

Bibtex @ { karaelmasfen805676, journal = {Karaelmas Fen ve Mühendislik Dergisi}, issn = {2146-7277}, address = {Zonguldak Bülent Ecevit Üniversitesi}, publisher = {Zonguldak Bülent Ecevit Üniversitesi}, year = {2018}, volume = {8}, number = {2}, pages = {590 - 594}, title = {Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi}, key = {cite}, author = {Sugeçti, Serkan and Büyükgüzel, Kemal} }
APA Sugeçti, S. & Büyükgüzel, K. (2018). Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi . Karaelmas Fen ve Mühendislik Dergisi , 8 (2) , 590-594 . Retrieved from https://dergipark.org.tr/tr/pub/karaelmasfen/issue/57117/805676
MLA Sugeçti, S. , Büyükgüzel, K. "Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi" . Karaelmas Fen ve Mühendislik Dergisi 8 (2018 ): 590-594 <https://dergipark.org.tr/tr/pub/karaelmasfen/issue/57117/805676>
Chicago Sugeçti, S. , Büyükgüzel, K. "Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi". Karaelmas Fen ve Mühendislik Dergisi 8 (2018 ): 590-594
RIS TY - JOUR T1 - Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi AU - Serkan Sugeçti , Kemal Büyükgüzel Y1 - 2018 PY - 2018 N1 - DO - T2 - Karaelmas Fen ve Mühendislik Dergisi JF - Journal JO - JOR SP - 590 EP - 594 VL - 8 IS - 2 SN - 2146-7277- M3 - UR - Y2 - 2022 ER -
EndNote %0 Karaelmas Fen ve Mühendislik Dergisi Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi %A Serkan Sugeçti , Kemal Büyükgüzel %T Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi %D 2018 %J Karaelmas Fen ve Mühendislik Dergisi %P 2146-7277- %V 8 %N 2 %R %U
ISNAD Sugeçti, Serkan , Büyükgüzel, Kemal . "Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi". Karaelmas Fen ve Mühendislik Dergisi 8 / 2 (Haziran 2018): 590-594 .
AMA Sugeçti S. , Büyükgüzel K. Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi. Karaelmas Fen ve Mühendislik Dergisi. 2018; 8(2): 590-594.
Vancouver Sugeçti S. , Büyükgüzel K. Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi. Karaelmas Fen ve Mühendislik Dergisi. 2018; 8(2): 590-594.
IEEE S. Sugeçti ve K. Büyükgüzel , "Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. Lepidoptera: Pyralidae Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi", Karaelmas Fen ve Mühendislik Dergisi, c. 8, sayı. 2, ss. 590-594, Haz. 2018