Phylogeny of Some Melanoleuca Species (Fungi: Basidiomycota) in Turkey and Identification of Melanoleuca angelesiana A.H. Sm. As a First Record
Öz
Aim of Study: The purposes of the present study are to describe
and identify all collected Melanoleuca
samples using morphological characters, determine phylogenetic relationships
among species using DNA sequences of ITS and LSU regions and check the number
of subgenera of Melanoleuca genus.
Area of Study: Samples were collected from different parts
of Turkey and the study was conducted at the Department of Molecular Biology
and Genetics in Van Yüzüncü Yıl University.
Material and
Methods: Twenty-two samples, representatives of fourteen species, were used.
Structures of pileus, stipe, lamellae and basidia, cystidia, spores were used
as macroscopic and microscopic features, respectively. The nuclear ribosomal
internal transcribed spacer region (nrITS) and largest subunit (LSU) were
amplified for molecular analyses.
Main results: Taxonomic
positions of some Melanoleuca species
from Turkey were evaluated based on both morphological characters and sequences
of two different nuclear DNA regions. nrITS region was more informative (112
variable sites in ITS1, 1 in 5.8S and 95 in ITS2 subregions) compared to LSU
(46 variable sites). Analyses of ITS region revealed that the genus Melanoleuca was monophyletic and divided
into two subgenera as Melanoleuca and Urticocystis.
Research
highlights: Within studied species, Melanoleuca
angelesiana was firstly reported for mycobiota of Turkey
Anahtar Kelimeler
Kaynakça
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