Yıl 2021, Cilt 21 , Sayı 1, Sayfalar 41 - 54 2021-04-03

Türk Göknar Taksonlarında Genetik Çeşitlilik Analizleri
Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa

Yasemin CELIK ALTUNOGLU [1] , Kerim GÜNEY [2] , Pınar BALOGLU [3] , Mehmet BALOGLU [4]


Çalışmanın amacı: Beş Abies taksonu Türkiye'de doğal olarak dağılmıştır. Abies nordmanniana' nın üç alt türü ve A. cilica 'nın iki alt türü vardır. Bu çalışmada, cpDNA bölgeleri, trnR-trnN ve rps18-rpl20 bölgelerine göre hem Türk taksonlarında hem de dünyanın diğer Abies taksonlarında filogenetik ilişkileri göstermeyi amaçladık. Materyal ve yöntem: CTAB metodu ile DNA izolasyonunun ardından ilgili göknar DNA bölgeleri çoğaltılmış ve dizilenmiştir. Filogenetik ağaçlar, 1000 tekrarlı maximum likelihood (maksimum olabilirlik) yöntemi kullanılarak oluşturulmuştur. Temel sonuçlar: Türkiye’deki göknar taksonları arasında bu bölgeler bakımından ayrım yapmak zor gözükmektedir. rps18-rpl20 dizileri temelindeki filogenetik ağaca dayanarak, bazı Abies cilicica subsp. isaurica, A. nordmanniana subsp. equi-trojani ve A. cilicica subsp. cilicica üyeleri A. spectabilis ve A. densa ile aynı sınıfta yer alırken, bazı A. nordmanniana subsp. bornmulleriana, A. cilicica subsp. isaurica ve A. nordmanniana subsp. equi-trojani üyeleri ise Türkiye’ye yakın yayılış gösteren A. alba ve Kuzey Amerika’da yayılışı bilinen A. amabilis ile aynı sınıfta yer almıştır. Araştırma Vurguları: Mevcut çalışma, Türkiye’deki göknar türleri arasında cpDNA çeşitliliğinin dağılımına ve Türkiye göknarları ile dünyada yayılış gösteren diğer göknar türleri arasındaki genetik çeşitliliğe yeni bir ışık tutmaktadır.
Aim of study: Five Abies taxa naturally distributed in Turkey. Abies nordmanniana has three subspecies and A. cilica has two subspecies. In this study, we aimed to show phylogenetic relationships both in Turkish taxa and in other Abies taxa from around the world based on cpDNA regions, trnR-trnN and rps18-rpl20 regions. Material and methods: Following CTAB-based DNA isolation method, the relevant fir DNA regions were amplified and sequenced. Phylogenetic trees were constructed using maximum likelihood method with 1000 bootstrap replicates. Main results: It was difficult to make distinctions among the Turkish Abies taxa based on the sequenced DNA regions. Based on rps18-rpl20 phylogenetic tree, some members of Abies cilicica subsp. isaurica, A. nordmanniana subsp. equi-trojani and A. cilicica subsp. cilicica were in the same clade with A. spectabilis and A. densa; However, some members of A. nordmanniana subsp. bornmuelleriana, A. cilicica subsp. isaurica and A. nordmanniana subsp. equi-trojani were placed in a clade with A. alba placed near Turkey and A. amabilis known from North America. Highlights: This study provides new insights into the distribution of cpDNA variation in Abies species in Turkey and the genetic variation between firs in Turkey and the rest of the world.
  • Aguirre-Planter, É., Jaramillo-Correa, J. P., Gómez-Acevedo, S., Khasa, D. P., Bousquet, J. & Eguiarte, L. E. (2012). Phylogeny, diversification rates and species boundaries of Mesoamerican firs (Abies, Pinaceae) in a genus-wide context. Molecular Phylogenetics and Evolution, 62(1), 263-274.
  • Ateş, M. A. (2011). Molecular phylogenetics of Turkish Abies (pinaceae) species based on matK gene regions of chloroplast genome (Master's thesis, Dept. of Biological Sciences, Middle East Technical University, Ankara).
  • Davis, P. (1985). PH 1965-1985. Flora of Turkey and the East Aegean Islands. 1-9. Edinburg: Edinburgh University Press.
  • Fady, B., Arbez, M. & Marpeau, A. (1992). Geographic variability of terpene composition in Abies cephalonica Loudon and Abies species around the Aegean: hypotheses for their possible phylogeny from the Miocene. Trees-Structure and Function, 6(3), 162-171.
  • Farjon, A. (1990). Pinaceae. Drawings and descriptions of the genera Abies, Cedrus, Pseudolarix, Keteleeria, Nothotsuga, Tsuga, Cathaya, Pseudotsuga, Larix and Picea. Königstein: Koeltz Scientific Books.
  • Gülbaba, A. G., Velioğlu, E., Özer, A. S., Doğan, B., Doerksen, A. H. & Adams, W. T. (1996). Population Genetic Structure of Kazdağı Fir (Abies equitrojani Aschers et Sint.), A Narrow Endemic to Turkey: Implications for in situ Conservation. In Proceedings of the International Symposium of In situ Conservation of Plant Genetic Diversity.
  • Güner, A., Özhatay, N., Ekim, T. & Başer, H. K. C. (2000). Flora of Turkey and East Aegean Islands: v.11 Supplement 2. Edinburgh: Edinburgh University Press.
  • Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M. (2012). Turkey plant list (vascular plants). İstanbul: Nezahat Gökyiğit Botanical Garden and Flora Research Association Publication.
  • Hansen, O. K., Kjær, E. D. & Vendramin, G. (2005). Chloroplast microsatellite variation in Abies nordmanniana and simulation of causes for low differentiation among populations. Tree Genetics & Genomes, 1(3), 116-123.
  • Hrivnák, M., Paule, L., Krajmerová, D., Kulaç, Ş., Şevik, H., Turna, İ., ... & Gömöry, D. (2017). Genetic variation in Tertiary relics: The case of eastern‐Mediterranean Abies (Pinaceae). Ecology and Evolution, 7(23), 10018-10030.
  • Isoda, K., Shiraishi, S. & Kisanuki, H. (2000). Classifying Abies species (Pinaceae) based on the sequence variation of a tandemly repeated array found in the chloroplast DNA trnL and trnF intergenic spacer. Silvae Genetica, 49(3), 161-164.
  • Kaya, Z., Skaggs, A. & Neale, D. B. (2008). Genetic Differentiation of Abies equi-trojani (Asch. & Sint. ex Boiss) Mattf. populations from Kazdağı, Turkey and the genetic relationship between Turkish Firs belonging to the Abies nordmanniana Spach Complex. Turkish Journal of Botany, 32(1), 1-10.
  • Kormutak, A., Vookova, B., Ziegenhagen, B., Kwon, H. Y. & Hong, Y. P. (2004). Chloroplast DNA variation in some representatives of the Asian, North American and Mediterranean firs (Abies spp). Silvae Genetica, 53(1-6), 99-104.
  • Letunic, I. & Bork, P. (2016). Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Research, 44(1), 242-245.
  • Li, H.L. (1975). Flora of Taiwan, Epoch publishing, 2, 316p., Taiwan.
  • Liang, H. (2003). The Phylogenetic Reconstruction of the grass family (Poaceae) using matK gene sequences (Doctoral dissertation, Virginia Polytechnic Institute, Blacksburg).
  • Özdemir Değirmenci, F. (2011). Molecular phylogenetic position of Turkish abies (pinaceae) based on noncoding trn regions of chloroplast genome (Master's thesis, Dept. of Biological Sciences, Middle East Technical University, Ankara).
  • Parducci, L. & Szmidt, A. (1999). PCR-RFLP analysis of cpDNA in the genus Abies. TAG Theoretical and Applied Genetics, 98(5), 802-808.
  • Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A. & Allard, R. (1984). Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences, 81(24), 8014-8018.
  • Semerikova, S.A., Semerikov, V.L. & Lascoux, M. (2011). Post‐glacial history and introgression in Abies (Pinaceae) species of the Russian Far East inferred from both nuclear and cytoplasmic markers. Journal of Biogeography, 38(2), 326-340.
  • Semerikova, S.A. & Semerikov, V.L. (2014). Molecular phylogenetic analysis of the genus Abies (Pinaceae) based on the nucleotide sequence of chloroplast DNA. Russian Journal of Genetics, 50(1), 7-19.
  • Suyama, Y., Yoshimaru, H. & Tsumura, Y. (2000). Molecular phylogenetic position of Japanese Abies (Pinaceae) based on chloroplast DNA sequences. Molecular Phylogenetics and Evolution, 16(2), 271-277.
  • Şimşek, Y. (1992). Türkiye Orijinli Göknar Türlerinin Genetik Yapıları Üzerine Araştırmalar. Ormancılık Araştırma Enstitüsü, Teknik Bülten Serisi (221).
  • Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729.
  • Tayanç, Y., Çengel, B., Kandemir, G. & Velioğlu, E. (2013). Türkiye’de Yayılış Gösteren Göknar (Abies spp.) Populasyonlarının Genetik Çeşitliliği ve Filogenetik Sınıflandırması. Orman Genel Müdürlüğü Orman Ağaçları ve Tohumları Islah Araştırma Enstitüsü Müdürlüğü Yayınları, (47).
  • Tozkar, C.Ö., Önde, S. & Kaya, Z. (2009). The phylogenetic relationship between populations of marginally and sympatrically located Pinus halepensis Mill. and Pinus brutia Ten. in Turkey, based on the ITS-2 region. Turkish Journal of Agriculture and Forestry, 33(4), 363-373.
  • Wang, X.R., Tsumura, Y., Yoshimaru, H., Nagasaka, K. & Szmidt, A.E. (1999). Phylogenetic relationships of Eurasian pines (Pinus, Pinaceae) based on chloroplast rbcL, matK, rpl20‐rps18 spacer, and trnV intron sequences. American Journal of Botany, 86(12), 1742-1753.
  • Xiang, Q.P., Xiang, Q.Y., Guo, Y.Y. & Zhang, X.C. (2009). Phylogeny of Abies (Pinaceae) inferred from nrITS sequence data. Taxon, 58(1), 141-152.
  • Xiang, Q.P., Xiang, Q.Y., Liston, A. & Zhang, X.C. (2004). Phylogenetic relationships in Abies (Pinaceae): evidence from PCR-RFLP of the nuclear ribosomal DNA internal transcribed spacer region. Botanical Journal of the Linnean Society, 145(4), 425-435.
  • Ziegenhagen, B., Fady, B., Kuhlenkamp, V. & Liepelt, S. (2005). Differentiating groups of Abies species with a simple molecular marker. Silvae Genetica, 54(1-6), 123-126.
Birincil Dil en
Konular Fen
Bölüm Makaleler
Yazarlar

Orcid: 0000-0003-2940-7464
Yazar: Yasemin CELIK ALTUNOGLU
Kurum: KASTAMONU ÜNİVERSİTESİ, MÜHENDİSLİK VE MİMARLIK FAKÜLTESİ, GENETİK VE BİYOMÜHENDİSLİK BÖLÜMÜ, GENETİK VE BİYOMÜHENDİSLİK PR.
Ülke: Turkey


Orcid: 0000-0003-2305-790X
Yazar: Kerim GÜNEY (Sorumlu Yazar)
Kurum: KASTAMONU ÜNİVERSİTESİ, ORMAN FAKÜLTESİ, ORMAN MÜHENDİSLİĞİ BÖLÜMÜ, ORMAN MÜHENDİSLİĞİ PR.
Ülke: Turkey


Orcid: 0000-0002-1575-8071
Yazar: Pınar BALOGLU
Kurum: KASTAMONU UNIVERSITY, RESEARCH CENTER FOR CENTRAL RESEARCH LABORATORY
Ülke: Turkey


Orcid: 0000-0003-2976-7224
Yazar: Mehmet BALOGLU
Kurum: KASTAMONU UNIVERSITY, FACULTY OF ENGINEERING-ARCHITECTURE, DEPARTMENT OF GENETICS AND BIOENGINEERING, GENETICS AND BIOENGINEERING PR.
Ülke: Turkey


Destekleyen Kurum Kastamonu University, Scientific Research Projects Coordination Department.
Proje Numarası KÜBAP-01/2012-11
Tarihler

Yayımlanma Tarihi : 3 Nisan 2021

Bibtex @araştırma makalesi { kastorman908630, journal = {Kastamonu University Journal of Forestry Faculty}, issn = {1303-2399}, eissn = {1309-4181}, address = {}, publisher = {Kastamonu Üniversitesi}, year = {2021}, volume = {21}, pages = {41 - 54}, doi = {10.17475/kastorman.908630}, title = {Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa}, key = {cite}, author = {Celık Altunoglu, Yasemin and Güney, Kerim and Baloglu, Pınar and Baloglu, Mehmet} }
APA Celık Altunoglu, Y , Güney, K , Baloglu, P , Baloglu, M . (2021). Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa . Kastamonu University Journal of Forestry Faculty , 21 (1) , 41-54 . DOI: 10.17475/kastorman.908630
MLA Celık Altunoglu, Y , Güney, K , Baloglu, P , Baloglu, M . "Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa" . Kastamonu University Journal of Forestry Faculty 21 (2021 ): 41-54 <https://dergipark.org.tr/tr/pub/kastorman/issue/61154/908630>
Chicago Celık Altunoglu, Y , Güney, K , Baloglu, P , Baloglu, M . "Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa". Kastamonu University Journal of Forestry Faculty 21 (2021 ): 41-54
RIS TY - JOUR T1 - Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa AU - Yasemin Celık Altunoglu , Kerim Güney , Pınar Baloglu , Mehmet Baloglu Y1 - 2021 PY - 2021 N1 - doi: 10.17475/kastorman.908630 DO - 10.17475/kastorman.908630 T2 - Kastamonu University Journal of Forestry Faculty JF - Journal JO - JOR SP - 41 EP - 54 VL - 21 IS - 1 SN - 1303-2399-1309-4181 M3 - doi: 10.17475/kastorman.908630 UR - https://doi.org/10.17475/kastorman.908630 Y2 - 2021 ER -
EndNote %0 Kastamonu Üniversitesi Orman Fakültesi Dergisi Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa %A Yasemin Celık Altunoglu , Kerim Güney , Pınar Baloglu , Mehmet Baloglu %T Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa %D 2021 %J Kastamonu University Journal of Forestry Faculty %P 1303-2399-1309-4181 %V 21 %N 1 %R doi: 10.17475/kastorman.908630 %U 10.17475/kastorman.908630
ISNAD Celık Altunoglu, Yasemin , Güney, Kerim , Baloglu, Pınar , Baloglu, Mehmet . "Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa". Kastamonu University Journal of Forestry Faculty 21 / 1 (Nisan 2021): 41-54 . https://doi.org/10.17475/kastorman.908630
AMA Celık Altunoglu Y , Güney K , Baloglu P , Baloglu M . Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa. Kastamonu University Journal of Forestry Faculty. 2021; 21(1): 41-54.
Vancouver Celık Altunoglu Y , Güney K , Baloglu P , Baloglu M . Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa. Kastamonu University Journal of Forestry Faculty. 2021; 21(1): 41-54.
IEEE Y. Celık Altunoglu , K. Güney , P. Baloglu ve M. Baloglu , "Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa", Kastamonu University Journal of Forestry Faculty, c. 21, sayı. 1, ss. 41-54, Nis. 2021, doi:10.17475/kastorman.908630