Türkiye'nin Kuzeydoğusundan Alınan Propolis Örneklerinin Antimikrobiyal, Anti-biyofilm, Anti-swarming, Anti-quorum Sensing Aktiviteleri ve Sitotoksisitesi

Yıl 2024, Cilt: 24 Sayı: 2, 158 - 167, 03.10.2024

Merve Cora , Ülkü Zeynep Üreyen Esertaş , İnci Durukan

https://doi.org/10.17475/kastorman.1557416

Öz

Çalışmanın amacı: Biyolojik aktif moleküller içerdiğinin anlaşılmasıyla birlikte propolis ile ilgili yapılan çalışmaların sayısı son yıllarda artmıştır. Bu çalışmada üç farklı Türkiye propolisinin etanol ekstraktlarının biyolojik aktiviteleri ve sitotoksik özelliklerinin araştırılması amaçlanmıştır.
Materyal ve yöntem: Ekstraktların antimikrobiyal özellikleri 14 mikroorganizma kullanılarak agar kuyucuk ve mikrodilüsyon yöntemleri ile araştırıldı. Anti-quorum sensing aktivitesi için Chromobacter violaceum, anti-swarming ve anti-biyofilm aktivite değerlendirilmesi için Pseudomonas aeruginosa PAO1 izolat kullanıldı. Ekstraktların sitotoksik etkileri MTT yöntemi ile pankreatik tümöral hücre hattı (AR42J), akciğer adenokarsinoma hücre hattı (A549), meme kanseri hücre ahttı (MDA-MB-231) ve normal epitel hücre hattı (Vero) kullanılarak araştırıldı.
Temel sonuçlar: Çalışmaya dahil edilen tüm ekstraktrın 8/14 mikroorganizmaya karşı etkili olduğu tespit edildi. Ayrıca tüm ekstraktlarda anti-quorum sensing aktivitesi görülürken, ekstraktlarda anti-swarming ve anti-biyofilm aktivitesi görülmedi. Tüm ekstraktların hücre hatları üzerine doza bağımlı sitotoksik etki gösterdiği belirlendi.
Araştırma vurguları: Çalışmada kullanılan propolis örneklerinin gösterdiği biyolojik aktivite nedeniyle gelecekte yeni ilaçların geliştirilmesinde rol oynayabileceği düşünülmektedir.

Anahtar Kelimeler

Biyofilm, MTT, Propolis, Sitotoksisite

Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye

Öz

Aim of study: Studies on propolis have increased as it has been revealed that it contains biologically active molecules. In the current study, it was aimed to analyze biological activity, and cytotoxicity of ethanolic extract of three different propolis samples from Türkiye.
Material and methods: The antibacterial activity of the extracts against 14 microorganisms was assessed using the agar well diffusion method and the microdilution method. Chromobacter violeceum was used in quorum-sensing assay, and Pseudomonas aeruginosa PAO1 strain was used in swarming and biofilm assays. Using the MTT test, the cytotoxic effect of the extracts was examined on the lung adenocarcinoma cell line (A549), pancreatic tumoral cell line (AR42J), breast cancer cell line (MDA-MB-231), and normal epithelial cell line (Vero).
Main results: All propolis extracts were effective against 8/14 microorganisms included in the study. While all propolis extracts have shown anti-quorum sensing activity, there was not any anti-swarming and anti-biofilm activity in each sample. It was demonstrated that every propolis sample had a dose-dependent cytotoxic effect on the examined cell lines.
Research highlights: Due to the biological activity shown by the propolis samples included in the study, it is considered that it has the potential to influence the creation of novel medications in the future.

Anahtar Kelimeler

Biofilm, Cytotoxicity, Propolis, Quorum sensing

Kaynakça

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