Araştırma Makalesi
BibTex RIS Kaynak Göster

Determination of Trace Elements, Heavy Metals Content, and Free Radical Scavenging Activity of the Pigments Extracted of Donax trunculus shells Gathered from Giresun Coast

Yıl 2022, , 584 - 592, 15.12.2022
https://doi.org/10.31466/kfbd.1075062

Öz

The study aimed to determine free radical scavenging activity, trace elements, and the heavy metals contents of the pigments extracted from Donax trunculus shells obtained at the Giresun the Black Sea coast. Free radical scavenging activity was determined by spectrophotometric DPPH and ABTS methods. Heavy metal and trace elements of the pigments were measured by ICP-MS. Pigments scavenged DPPH and ABTS radicals. On the other hand, it determined that the pigments had rich trace element concentrations (Fe 90.78 0.01 mg/kg; Cr 71.630.01; mg/kg, Mn 40.460.01 mg/kg; Zn 5.130.01 mg/kg; Cu 3.690.01 mg/kg; Se 1.220.01 mg/kg). Heavy metal content was within acceptable limits (Ni 4.280.03 mg/kg; Co 0.670.02 mg/kg; Pb 0.690.01 mg/kg; Cd 0.3880.01 mg/kg; As 0.2840.02 mg/kg). These results suggested that D. trunculus shells, considered food waste, would be a new bio-resource for obtaining natural antioxidants and micronutrients.

Kaynakça

  • Abdel-Latif, H. M., Dawood, M. A., Menanteau-Ledouble, S., & El-Matbouli, M. (2020). Environmental transformation of n-TiO2 in the aquatic systems and their ecotoxicity in bivalve mollusks: A systematic review. Ecotoxicology and Environmental Safety, 200, 110776. https://doi.org/10.1016/j.ecoenv.2020.110776.
  • Aydin, M., Tunca, E., & Ersoy, N. E. (2020). Morphometric aspects and growth parameters of the wedge clam (Donax trunculus) of the Black Sea, Turkey. Journal of Anatolian Environmental and Animal Sciences, 5(1), 11-18. doi: 10.35229/jaes.637729 %U 10.35229/jaes.637729
  • Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5.
  • Buha, A., Baralić, K., Djukic-Cosic, D., Bulat, Z., Tinkov, A., Panieri, E., & Saso, L. (2021). The role of toxic metals and metalloids in nrf2 signaling. Antioxidants, 10(5), 630. https://doi.org/10.3390/antiox10050630
  • Godswill, A. G., Somtochukwu, I. V., Ikechukwu, A. O., & Kate, E. C. (2020). Health benefits of micronutrients (vitamins and minerals) and their associated deficiency diseases: A systematic review. International Journal of Food Sciences, 3(1), 1-32. https://doi.org/10.47604/ijf.1024
  • Gudjoncik, A., Guenancia, C., Zeller, M., Cottin, Y., Vergely, C., & Rochette, L. (2014). Iron, oxidative stress, and redox signaling in the cardiovascular system. Molecular nutrition & food research, 58(8), 1721-1738. https://doi.org/10.1002/mnfr.201400036
  • Kuwahara, R., Hatate, H., Yuki, T., Murata, H., Tanaka, R., & Hama, Y. (2009). Antioxidant property of polyhydroxylated naphthoquinone pigments from shells of purple sea urchin Anthocidaris crassispina. LWT-Food Science and Technology, 42(7), 1296-1300. https://doi.org/10.1016/j.lwt.2009.02.020.
  • Künili, İ. E., Çolakoğlu, S., & Çolakoğlu, F. (2021). Levels of PAHs, PCBs, and toxic metals in Ruditapes philippinarum and Donax trunculus in Marmara Sea, Turkey. Journal of the Science of Food and Agriculture, 101(3), 1167-1173. https://doi.org/10.1002/jsfa.10728
  • Lauritano, C., & Ianora, A. (2020). Chemical defense in marine organisms. Marine Drugs, 18(10), 518. https://doi.org/10.3390/md18100518
  • Le Saux, A., David, E., Betoulle, S., Bultelle, F., Rocher, B., Barjhoux, I., & Cosio, C. (2020). New insights into cellular impacts of metals in aquatic animals. Environments, 7(6), 46. https://doi.org/10.3390/environments7060046
  • Liu, R., & Mabury, S. A. (2020). Synthetic phenolic antioxidants: A review of environmental occurrence, fate, human exposure, and toxicity. Environmental science & technology, 54(19), 11706-11719. doi: 10.1021/acs.est.0c05077
  • Pontoni, L., La Vecchia, C., Boguta, P., Sirakov, M., D’Aniello, E., Fabbricino, M., & Locascio, A. (2021). Natural organic matter controls metal speciation and toxicity for marine organisms: a review. Environmental Chemistry Letters, 1-16. https://doi.org/10.1007/s10311-021-01310-y
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237.
  • Roussiez, V., Probst, A., & Probst, J. L. (2013). Significance of floods in metal dynamics and export in a small agricultural catchment. Journal of hydrology, 499, 71-81. https://doi.org/10.1016/j.jhydrol.2013.06.013.
  • Strain, J. S., & Cashman, K. D. (2009). Minerals and trace elements. Introduction to human nutrition, 188.
  • Strehse, J. S., & Maser, E. (2020). Marine bivalves as bioindicators for environmental pollutants with focus on dumped munitions in the sea: A review. Marine environmental research, 158, 105006. https://doi.org/10.1016/j.marenvres.2020.105006.
  • Zamani-Ahmadmahmoodi, R., Malekabadi, M. B., Rahimi, R., & Johari, S. A. (2020). Aquatic pollution caused by mercury, lead, and cadmium affects cell growth and pigment content of marine microalga, Nannochloropsis oculata. Environmental Monitoring and Assessment, 192(6), 1-11. https://doi.org/10.1007/s10661-020-8222-5.
  • URL-1. https://www.efsa.europa.eu/sites/default/files/efsa_rep/blobserver_assets/ndatolerableuil.pdf (Date Accessed:17.05.2022).
  • URL-2. https://www.efsa.europa.eu/en/topics/topic/metals-contaminants-food (Date Accessed:17.05.2022).

Giresun Sahilinden Toplanan Donax trunculus Kabuklarından Elde Edilen Pigmentlerin İz Element, Ağır Metal İçeriği ve Serbest Radikal Giderme Aktivitelerinin Belirlenmesi

Yıl 2022, , 584 - 592, 15.12.2022
https://doi.org/10.31466/kfbd.1075062

Öz

Bu çalışma, Giresun’un Karadeniz kıyılarından toplanmış Donax trunculus kabuklarından elde edilen pigmentlerin serbest radikal temizleme aktivitesini, eser element ve ağır metal içeriklerini belirlemeyi amaçladı. Serbest radikal temizleme aktivitesi, spektrofotometrik DPPH ve ABTS yöntemleri ile belirlendi. Pigmentlerin ağır metal ve eser element içerikleri ICP-MS ile ölçüldü. Pigmentler DPPH ve ABTS radikallerini temizledi. Öte yandan pigmentlerin zengin eser element konsantrasyonlarına sahip olduğu belirlendi (Fe 90.78 0.01 mg/kg; Cr 71.630.01; mg/kg, Mn 40.460.01 mg/kg; Zn 5.130.01 mg/kg; Cu 3.690.01 mg/kg; Se 1.220.01 mg/kg). Ağır metal içeriği kabul edilebilir sınırlar içindeydi (Ni 4.280.03 mg/kg; Co 0.670.02 mg/kg; Pb 0.690.01 mg/kg; Cd 0.3880.01 mg/kg; As 0.2840.02 mg/kg). Bu sonuçlar, gıda atığı olarak kabul edilen D. trunculus kabuklarının, doğal antioksidanlar ve mikro besinler elde etmek için yeni bir biyolojik kaynak olacağını sergilemiştir.

Kaynakça

  • Abdel-Latif, H. M., Dawood, M. A., Menanteau-Ledouble, S., & El-Matbouli, M. (2020). Environmental transformation of n-TiO2 in the aquatic systems and their ecotoxicity in bivalve mollusks: A systematic review. Ecotoxicology and Environmental Safety, 200, 110776. https://doi.org/10.1016/j.ecoenv.2020.110776.
  • Aydin, M., Tunca, E., & Ersoy, N. E. (2020). Morphometric aspects and growth parameters of the wedge clam (Donax trunculus) of the Black Sea, Turkey. Journal of Anatolian Environmental and Animal Sciences, 5(1), 11-18. doi: 10.35229/jaes.637729 %U 10.35229/jaes.637729
  • Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5.
  • Buha, A., Baralić, K., Djukic-Cosic, D., Bulat, Z., Tinkov, A., Panieri, E., & Saso, L. (2021). The role of toxic metals and metalloids in nrf2 signaling. Antioxidants, 10(5), 630. https://doi.org/10.3390/antiox10050630
  • Godswill, A. G., Somtochukwu, I. V., Ikechukwu, A. O., & Kate, E. C. (2020). Health benefits of micronutrients (vitamins and minerals) and their associated deficiency diseases: A systematic review. International Journal of Food Sciences, 3(1), 1-32. https://doi.org/10.47604/ijf.1024
  • Gudjoncik, A., Guenancia, C., Zeller, M., Cottin, Y., Vergely, C., & Rochette, L. (2014). Iron, oxidative stress, and redox signaling in the cardiovascular system. Molecular nutrition & food research, 58(8), 1721-1738. https://doi.org/10.1002/mnfr.201400036
  • Kuwahara, R., Hatate, H., Yuki, T., Murata, H., Tanaka, R., & Hama, Y. (2009). Antioxidant property of polyhydroxylated naphthoquinone pigments from shells of purple sea urchin Anthocidaris crassispina. LWT-Food Science and Technology, 42(7), 1296-1300. https://doi.org/10.1016/j.lwt.2009.02.020.
  • Künili, İ. E., Çolakoğlu, S., & Çolakoğlu, F. (2021). Levels of PAHs, PCBs, and toxic metals in Ruditapes philippinarum and Donax trunculus in Marmara Sea, Turkey. Journal of the Science of Food and Agriculture, 101(3), 1167-1173. https://doi.org/10.1002/jsfa.10728
  • Lauritano, C., & Ianora, A. (2020). Chemical defense in marine organisms. Marine Drugs, 18(10), 518. https://doi.org/10.3390/md18100518
  • Le Saux, A., David, E., Betoulle, S., Bultelle, F., Rocher, B., Barjhoux, I., & Cosio, C. (2020). New insights into cellular impacts of metals in aquatic animals. Environments, 7(6), 46. https://doi.org/10.3390/environments7060046
  • Liu, R., & Mabury, S. A. (2020). Synthetic phenolic antioxidants: A review of environmental occurrence, fate, human exposure, and toxicity. Environmental science & technology, 54(19), 11706-11719. doi: 10.1021/acs.est.0c05077
  • Pontoni, L., La Vecchia, C., Boguta, P., Sirakov, M., D’Aniello, E., Fabbricino, M., & Locascio, A. (2021). Natural organic matter controls metal speciation and toxicity for marine organisms: a review. Environmental Chemistry Letters, 1-16. https://doi.org/10.1007/s10311-021-01310-y
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237.
  • Roussiez, V., Probst, A., & Probst, J. L. (2013). Significance of floods in metal dynamics and export in a small agricultural catchment. Journal of hydrology, 499, 71-81. https://doi.org/10.1016/j.jhydrol.2013.06.013.
  • Strain, J. S., & Cashman, K. D. (2009). Minerals and trace elements. Introduction to human nutrition, 188.
  • Strehse, J. S., & Maser, E. (2020). Marine bivalves as bioindicators for environmental pollutants with focus on dumped munitions in the sea: A review. Marine environmental research, 158, 105006. https://doi.org/10.1016/j.marenvres.2020.105006.
  • Zamani-Ahmadmahmoodi, R., Malekabadi, M. B., Rahimi, R., & Johari, S. A. (2020). Aquatic pollution caused by mercury, lead, and cadmium affects cell growth and pigment content of marine microalga, Nannochloropsis oculata. Environmental Monitoring and Assessment, 192(6), 1-11. https://doi.org/10.1007/s10661-020-8222-5.
  • URL-1. https://www.efsa.europa.eu/sites/default/files/efsa_rep/blobserver_assets/ndatolerableuil.pdf (Date Accessed:17.05.2022).
  • URL-2. https://www.efsa.europa.eu/en/topics/topic/metals-contaminants-food (Date Accessed:17.05.2022).
Toplam 19 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Ayşegül Çebi 0000-0003-3804-7966

Emine Gülçeri Güleç Peker 0000-0001-7244-0281

İspandiyar Rustamzada Bu kişi benim 0000-0002-0597-1514

Elif Soylu 0000-0002-7583-3416

Yayımlanma Tarihi 15 Aralık 2022
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Çebi, A., Güleç Peker, E. G., Rustamzada, İ., Soylu, E. (2022). Determination of Trace Elements, Heavy Metals Content, and Free Radical Scavenging Activity of the Pigments Extracted of Donax trunculus shells Gathered from Giresun Coast. Karadeniz Fen Bilimleri Dergisi, 12(2), 584-592. https://doi.org/10.31466/kfbd.1075062