Repurposing Pheophorbide a as an Antiviral Molecule for Human Papillomavirus type 16

Yıl 2025, Cilt: 15 Sayı: 2, 745 - 763, 15.06.2025

Ragıp Soner Silme

https://doi.org/10.31466/kfbd.1607109

Öz

The emergence of human papillomavirus (HPV) among the human population in different regions of the world has heightened the interest for new treatment methods. Molecular docking is a reliable and predictive method for screening of new potential antiviral molecules against pathogens. The retrieved 42 high-quality whole-genome sequences of HPV were analyzed in search of a stable region which is exist in the genome. The protein encoded by this stable region was targeted for protein-ligand interaction. Consequently, a target constant region which is responsible for encoding the C-terminal domain (monomer) of the HPV45 oncoprotein E7 (PDB: 2ewl) with various regulatory functions for the virus was detected. A ligand “Pheophorbide a (C35H36N4O5)” interacts with this protein, which could be used against HPV. Pheophorbide a has also been commercially used for cancer treatments due to its anti-proliferative effects and photosensitizer property in photodynamic therapy. This possible antiviral property of Pheophorbide a should be tested on other high-risk and low-risk Papillomaviruses since the E7 oncoprotein also plays a central role in many other HPV types. The predictive results need confirmation with further clinical and in vitro studies. The findings will provide new insights into HPV-human cell interactions, induced immunity, and new methods for HPV treatment.

Anahtar Kelimeler

Antiviral , Anticancer , HPV , Human papillomavirus , Pheophorbide a

Etik Beyan

The author declares that this study complies with Research and Publication Ethics.

Destekleyen Kurum

The author received no financial support for the research, authorship, and publication of this article.

Teşekkür

This article is dedicated to HPV patients. The author gratefully thanks to Prof. Dr. Ömür Baysal for his comments and suggestions. The sequence data of this study are openly available in the NCBI database. Supplementary materials are available online: https://doi.org/10.6084/m9.figshare.28028489

Feoforbid a'nın İnsan Papilloma Virüsü Tip 16'ya Karşı Kullanımı

Öz

Dünyanın farklı bölgelerindeki insan popülasyonunda insan papillomavirüsünün (HPV) ortaya çıkması, yeni tedavi yöntemlerine olan ilgiyi artırmıştır. Moleküler yerleştirme, patojenlere karşı yeni potansiyel antiviral moleküllerin taranması için güvenilir ve öngörücü bir yöntemdir Yüksek kaliteli 42 HPV tüm genom dizisi, genomda sabit bir bölgenin varlığı açısından analiz edildi. Bu sabit bölge tarafından kodlanan protein, protein-ligand etkileşimi için analiz edildi. Sonuç olarak, virüs bünyesinde çeşitli düzenleyici işlevlere sahip HPV45 oncoprotein E7’nin C-terminal domaini (monomer) (PDB: 2ewl) proteinini kodlamaktan sorumlu bir sabit bölge tespit edildi. HPV'ye karşı kullanılabilecek bir ligand olan "Feoforbid a'nın (C35H36N4O5)" bu proteinle etkileşime girdiği öngörülmüştür. Feoforbid a, fotodinamik terapide anti-proliferatif etkileri ve fotosensitizör özelliği nedeniyle ticari olarak kanser tedavilerinde de kullanılmaktadır. Feoforbid a'nın bu kuvvetle muhtemel antiviral özelliği, E7 onkoproteini birçok farklı HPV tipinde de merkezi bir rol oynadığı için, diğer yüksek riskli ve düşük riskli Papillomavirüsler üzerinde test edilmelidir. Bu sonuçların daha ileri klinik ve in vitro çalışmalar ile doğrulanması gerekmektedir. Bulgular, HPV-insan hücre etkileşimleri, indüklenen bağışıklık ve HPV tedavisinde geliştirilecek yöntemler konusunda yeni fikirler sağlayabilir.

Anahtar Kelimeler

Antiviral , Antikanser , HPV , İnsan papilloma virüsü , Feoforbid a

Kaynakça

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