Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts

Yıl 2019, , 176 - 185, 30.06.2019

Hatice Aysun Mercimek Takcı , Filiz Ucan Turkmen Fatma Ceren Anlas Fulya Ustun Alkan , Pemra Bakırhan , Cihan Demir Nazım Sekeroglu

https://doi.org/10.31466/kfbd.566276

Cited By: 3

Öz

Alkanna tinctoria (L.) Tausch produced naphthoquinones known as
DNA-topoisomerases inhibitors has been used as the traditional therapeutic agent, especially wound healing in
Turkey. The antimicrobial activity against 9 clinical microorganisms and
cytotoxicity on canine mammary carcinoma cell line (CMT-U27) of Alkanna tinctoria root extracts (methanol,
ethanol and acetonitrile) were researched by using MTT analysis. Alkanet root extracts showed antimicrobial activity
against 2 (Proteus spp. and S. haemolyticus)
out of 9 microorganisms. In vitro study towards CMT-U27 cancer cells, acetonitrile
root extract at 100 µg/mL concentration showed
strong and significant anti-proliferative effect. Our
results are manifested that the Alkanet acetonitrile root extract can be
evaluated as potential therapeutic agent, immediately after identification of bio-active metabolits in extract.

Anahtar Kelimeler

Alkanet root, Alkanna tinctoria, antimicrobial, cytotoxicity

Alkanna Tinctoria (L.) Tausch Kök Ekstraktlarının Antimikrobiyal Aktivitesi ve Sitotoksisitesi

Öz

DNA-topoizomerazları
olarak bilinen naftokinonları üreten Alkanna
tinctoria (L.) Tausch, Türkiye’de özellikle yara iyileştirmede geleneksel
terapötik ajan olarak kullanılmaktadır. Alkanna tinctoria metanol, etanol
ve asetonitril kök ekstraktlarının 9 klinik mikroorganizmaya karşı
antimikrobiyal aktivitesi ve köpek meme kanser hücre hattı (CMT-U27) üzerinde
sitotoksititesi MTT analizi kullanılarak araştırılmıştır. Alkanat kök
ekstraktları 9 mikroorganizmadan sadece ikisine (Proteus spp. and S. haemolyticus) karşı antimikrobiayl
aktivite göstermiştir. CMT-U27 kanser hücrelerine yönelik in vitro çalışmada, 100 µg/mL
konsantrasyondaki asetonitril kök ekstraktı güçlü ve önemli bir hücre
büyümesini engelleyici etki göstermiştir. Sonuçlarımız Alkanat asetonitril kök ekstraktının biyoaktif
metabolitlerinin tanımlanmasını takiben potansiyel terapötik ajan olarak
değerlendirilebileceğini açıkça ifade etmektedir.

Anahtar Kelimeler

Alkanat kök, Alkanna tinctoria, antimikrobiyal, sitotoksisite

Kaynakça

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