Effects of incubation temperature (25-49C°), initiation pH of media (5-10) and incubation time (6-24 h) independent variables for enhancing antibacterial activities of erythromycin (15 µg/disc), chloramphenicol (30 µg/disc), streptomycin (10 µg/disc) and tetracycline (30 µg/disc) standard antibiotics on Klebsiella spp.were optimized by using Box Behnken design model. Antibacterial analyses were performed by Kirby-Bauer disc diffusion method. A positive correlation was found between antibacterial activity and tested independent variables for erythromycin and streptomycin antibiotics. The increases of incubation temperature, pH and incubation time in the analyzed model were caused to the increase in antibacterial activity against Klebsiella spp. The highest activity for erythromycin was 22.50 mm. This activity was observed in two different optimization testing (at 49C°; pH 7.5 and 6 h; 25C°; pH 10.00 and 15 h). The highest activity (27.00 mm) for streptomycin was recorded at 25C°; pH 10.00 and 15 h. According to variance analyses, this model was statistically significant in enhancing activities of erythromycin and streptomycin (p˂0.05), but statistically insignificant for chloramphenicol and tetracycline. The effect of incubation temperature on streptomycin activity and incubation time on erythromycin activity was statistically significant found (p˃0.05). The interactions of incubation temperature and pH factors on activity of both antibiotics were detected to be statistically significant.
Birincil Dil | Türkçe |
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Bölüm | Makaleler |
Yazarlar | |
Yayımlanma Tarihi | 15 Haziran 2020 |
Yayımlandığı Sayı | Yıl 2020 |
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