Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae)
Öz
Reducing the usage of chemical pesticides used to control pests in agriculture and stored products is an important phenomenon. Also, eco-friendly biopesticides have become highly important for use in the management of the pest control programs to overcome the negative influences of other synthetic chemicals. In this study oxidative stress changes induced by one important phytochemical compound, juglone, in Galleria mellonella larvae were investigated. For this purpose, effective concentrations (LC10: 0.5 mg; LC30: 1.5 mg; LC50: 2.3 mg) of juglone were added into the 2 g diet of the first instar larvae. Hemolymph samples from the last instar larvae were used to analyses of antioxidant enzyme activity (glutathione peroxidases – GPx, glutathione-S transferase - GST, catalase - CAT, and superoxide dismutase - SOD) and malondialdehyde (MDA) assays. Activities of antioxidant enzymes (SOD, CAT, and GST) and MDA level in the hemolymph of last instar G. mellonella larvae exposed to dietary juglone changed dose-dependently when compared to the control larvae. However, there was no change detected in GPx enzyme activity in both treated and untreated larvae. Consequently, effective doses of juglone are toxic to the model and pest insect G. mellonella.
Anahtar Kelimeler
Galleria mellonella, Antioxidant Metabolism, Hemolymph, Juglone
Kaynakça
- Akhtar, Y., Isman, M. B., Niehaus, L. A., Lee, C. H. and Lee, H. S. (2012). Antifeedant and toxic effects of naturally occurring and synthetic quinones to the cabbage looper, Trichoplusia ni. Crop Protection, 31, 8-14.
- Altuntaş, H. (2015a). Determination of gibberellic acid (GA3)-induced oxidative stress in a model organism Galleria mellonella L. (Lepidoptera: Pyralidae). Environmental Entomology (Physiology, 44 (1), 100-105.
- Altuntaş, H. (2015b). Effects of ethephon on the hemolymph metabolites of the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae). Acta Physica Polonica A, 128, 182-183.
- Altuntaş, H., Duman, E. and Kılıç, G. (2020). Juglone induced oxidative and genotoxic stress in the model insect Galleria mellonella L. (Pyralidae: Lepidoptera). International Journal of Tropical Insect Science, 40, 611-619
kapsamında lisanslanmıştır.