Sakarya Nehir Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Bitki Gelişim Teşvik Edici Özelliklerinin Belirlenmesi

Yıl 2021, , 239 - 256, 15.06.2021

Uğur Çiğdem , Ayten Kumaş Fadime Özdemir Koçak

https://doi.org/10.31466/kfbd.889423

Öz

Biyoaktif bileşik üretim potansiyeli yüksek olan aktinobakteriler antibiyotik, antitümör ajanı, bitki gelişimini teşvik eden faktörler ve enzimler üretebilmektedirler. Yeni biyoaktif bileşiklerin keşfi için faklı ekstrem ortamlardan izolasyon çalışmaları yapılmaktadır. Bu çalışmada, Sakarya Nehir kaynağının sedimentinden ilk kez aktinobakteri izolasyonu ve bu bakterilerin ürettiği farklı bioaktif metabolitlerin varlığı araştırlmıştır. Antimikrobiyal aktivite deneylerinde Gram pozitif, Gram negatif bakteriler, maya ve funguslar kullanılmıştır. İzolatların azotu (N) fikse edebilme inorganik fosfatı çözebilme yeteneklerine, indol asetik asit (IAA) üretebilme ve kazeinaz aktivitelerine bakılmıştır. 17 aktinobakteri izolatının 16S rDNA analizleri sonucunda, izolatlar Micromonospora sp., (14), Saccharomonospora sp. (2) ve Cellulomonas sp. (1) olarak tanımlanmıştır. Elde edilen sonuçlarda, Micromonospora izolatlarının Gram pozitif bakterilere, maya ve funguslara karşı etkin olduğu belirlenmiştir. 12 izolatın N’u fikse edebildiği, 7 izolatın IAA üretebildiği, 2 izolatın kazeinaz aktivitesine sahip olduğu görülmüştür. Antimikrobiyal özellikleri açısından yüksek aktiviteye sahip olanların iyi birer farmasötik aday olabileceği ve bitki gelişimini teşvik edici potansiyele sahip izolatların da tarım alanında biyogübre olarak kullanım potansiyeline sahip olduğu düşünülmektedir. Ayrıca, 16S rDNA dizi analizleri sonucunda 2 Micromonospora, Saccharomonospora sp. ve Cellulomonas sp. Suşlarının yeni birer tür olması söz konusudur.

Anahtar Kelimeler

Aktinobakteriler, Antimikrobiyal aktivite, Bitki gelişimini teşvik özellikleri, 16S rDNA analizi

Destekleyen Kurum

Bilecik Şeyh Edebali Üniversitesi

Proje Numarası

2016-01.BŞEÜ.13-01

Teşekkür

Bu çalışma Bilecik Şeyh Edebali Üniversitesi BAP tarafından 2016-01.BŞEÜ.13-01.kodlu proje ile desteklenmiştir.

Determination of Antimicrobial and Plant Growth Promoting Properties of Actinobacteria Isolated from Sakarya River Sediment

Öz

Actinobacteria with high bioactive compound production potential can produce antibiotics, antitumor agents, factors that promote plant growth and enzymes. Isolation studies from different extreme environments are carried out for the discovery of new bioactive compounds. In this study, isolation of actinobacteria from the sediment of the headwaters of the River Sakarya and screening of their different bioactive metabolites were firstly performed. Gram positive, Gram negative bacteria, yeast and fungi were used in antimicrobial activity assay. The ability to (N) fix nitrogen, to dissolve inorganic phosphate, to produce indole acetic acid (IAA), and caseinase activities of isolates were investigated. As a result of 16S rDNA analysis of 17 actinobacteria isolates, the isolates were defined as Micromonospora sp., (14) as Saccharomonospora sp. (2) and as Cellulomonas sp. (1). In the results obtained, it was revealed that Micromonospora isolates have effective activity against Gram positive, yeast and fungi. 7 of the isolates could produce IAA and 12 of them could fix N and while 2 of the isolates have caseinase activity. The results obtained demostrated that those with high activity in terms of antimicrobial properties will can good pharmaceutical candidates. Isolates that have the potential to stimulate plant growth are also thought to have the potential to be used as biofertilizers in agriculture. In addition, as a result of 16S rDNA sequence analysis, it is possible that 2 Micromonospora sp., Saccharomonospora sp. and Cellulomonas sp. strains are new species.

Anahtar Kelimeler

Actinobacteria, Antimicrobial activity, Plant growth promoting, 16S rDNA analysis

Proje Numarası

2016-01.BŞEÜ.13-01

Kaynakça

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