Elastaz Aktivitesine Giresun Yöresindeki Bazı Yenilebilir Bitkilerin Farklı Çözücülerdeki Ekstrelerinin İnhibisyon Etkilerinin İncelenmesi

Yıl 2017, Cilt: 7 Sayı: 2, 10 - 18, 15.12.2017

Bahar Bilgin Sökmen , Yasemin Sağkal

https://doi.org/10.31466/kfbd.311178

Cited By: 1

Öz

Elastazlar (E.C.
3.4.21.36), bağ dokunun önemli bir proteni olan elastini ayırma özelliğine
sahip bir grup serin proteazlardır. Bu çalışmada, Giresun yöresinde yetişen 15
çeşit yenilebilir bitkinin farklı çözücülerdeki ekstrelerinin, elastaz enzim
aktivitesi üzerindeki inhibitör etkisi incelendi. Bu amaçla, su, metil alkol ve
etil asetat ile hazırlanan ekstrelerinin elastaz aktivitesi üzerine inhibitör
etkileri spektrofotometrik olarak analiz edildi. Bu bitkiler arasında, kara
lahananın (Brassica oleracea) sulu ekstresinin (IC₅₀= 0,0002 ± 0,0001 µg/mL), söğüt otunun (Polygonum lapathifolium)
metil alkollü ekstresinin (IC₅₀= 0,0003 ± 0,0002 µg/mL) ve mendeğin (Aegopodium podagraria)
etil asetatlı ekstresinin (IC₅₀=0,0007 ± 0,0001 µg/mL) elastaz enzimine karşı en yüksek inhibitör
etkisine sahip olduğu bulundu. Analizlerin sonucunda, bitki ekstrelerinin elastaz
üzerinde farklı inhibitör etkilerine sahip oldukları belirlendi. Çalışmada elde
edilen sonuçlar, çalışılan bitkilerin alternatif bir anti-elastaz kaynağı olarak
kullanılabileceğini açıkça göstermektedir.

Anahtar Kelimeler

Elastaz, Enzim, Enzim İnhibisyonu, İnhibitör, Bitki

Investigation of the Inhibition Effetcs of Extracts in Different Solvents of Some Edible Plants in the Region of Giresun on Elastase Activity

Öz

Elastases (Porcine
pancreatic elastase, E.C. 3.4.21.36) are a group of serine proteases
which possess the ability to cleave the important connective tissue protein elastin. In this study,
inhibitory effects on elastase activity of the extracts in different solvents
of 15 kinds of edible plants growing in Giresun province were investigated. For
this purpose, inhibitory effects on elastase activity of the plants extracts
which prepared with water, methyl alcohol and ethyl acetate were analysed
spectrophotometrically. Among
the plants , water extract of Brassica
oleracea (IC₅₀ = 0.0002 ± 0.0001 µg/mL); methyl
alcohol extract of Polygonum lapathifolium (IC₅₀ = 0.0003 ±
0.0002 µg/mL) and ethyl acetate extract of Aegopodium podagraria (IC₅₀
= 0.0007 ± 0.0001 µg/mL) were found that they are the highest inhibitory
effect against elastase enzyme. As
a result of analysis, it was determined that the plant extracts showed
different inhibitory effects on elastase. The data acquired in this study
clearly shows that studied plants might be utilised as an alternative
anti-elastase source.

Anahtar Kelimeler

Elastase, Enzyme, Enzyme Inhibition, Inhibitory, Plant

Kaynakça

• Alasbahi, R. and Melzig, M. (2008). The in vitro Inhibition of Human Neutrophil Elastase Activity by some Yemeni Medicinal Plants. 2008. Scienta Pharmaceutica, 76, 471-483. Antonicelli, F., Bellon, G., Debelle, L. and Horneback, W. (2007). Elastin-elastases and inflamm-aging. Current Topics in Developmental Biology, 79, 99-155. Daamen, W. F., Verkamp, J. H., Van Hest, J. C. M. and Kuppevelt, T. H. (2007). Elastinas a biomaterial for tissue engineering. Biomaterials, 28, 4378-4398. Dinç, Y. (2009). Üreaz Enziminin Bazı Tıbbi Bitkiler Tarafından İnhibisyonu. Yüksek Lisans Tezi, İstanbul Üniversitesi, Fen Bilimleri Enstitüsü, İstanbul. Ho, C. T., Ferraro, T., Chen, Q., Rosen, R. T. and Huang M. T. (1994). Phytochemicals in Teas and Rosemary and Their Cancer-Preventive Properties. Food Phytochemicals for Cancer Prevention. II. Tea, Spices and Herbs. (Eds) Ho, C.-T., Osawa, T., Huang, M. T., Rosen, R. T. ACS Symposium Series 547. Washington DC, American Chemical Society, 2-19. Kalaycıoğlu, A., Öner, C. (1994). Bazı Bitki Ekstraktlarının Antimutajenik Etkilerinin Amest-salmonella Test Sistemi İle Araştırılması. The Turkish Journal of Botany, 18, 117-122. Kim, J. H., Byun, J. C., Bandi, A. K. R., Hyun, C. G. and Lee, N. H. (2009). Compounds with Elastase Inhibition and Free Radical Scavenging Activities from Callistemon lanceolatus. Journal of Medicinal Plants Research, 3, 914-920. Koca, İ., Hasbay, İ. ve Bostancı, Ş. (2011, Ekim). Samsun ve Çevresinde Sebze Olarak Kullanılan Bazı Yabani Bitkiler ve Tüketim Şekilleri. Samsun Sempozyumu, Samsun. Lee, K. K., Choy, J. J., Park, E. J. and Choiy, J. D. (2001). Anti-Elastase and Anti-Hyaluronidase of Phenolic Substance from Areca Catechu as A New Anti-Ageing Agent. International Journal of Cosmetic Science, 23, 341-346. Masuda, M., Murata, K., Fukuhama, A., Naruto, S., Fujita,T., Uwaya, A., Isami, F. and Matsuda, H. (2009). Inhibitory Effects of Constituents of Morinda citrifolia Seeds on Elastase and Tyrosinase. Journal of Natural Medicines, 63, 267-273. Matsubara, S., Shibata, H., Ishikawa, F., Yokokura, T., Takahashi, M., Sugimura, T., Wakabayashi, K. (2003). Suppression of Helicobacter pylori induced gastiritis by green tea extract in mongolian gerbils. Biochemical and Biophysical Research Communications, 310, 715-719. Moon, J.Y., Yim, E.Y., Song, G., Lee, N.H. and Hyun, C.G. 2010. Screening of elastase and tyrosinase inhibitory activity from Jeju Island plants. Eur Asian Journal of Bio Sciences, 4, 41-53. Nar, H., Werle, K., Bauer, M. M. T., Dollinger, H. and Jung, B. (2001). Crystal structure of human macrophage elastase (MMP-12) in complex with a hydroxamic acid inhibitor. Journal of Molecular Biology 312, 743 -751. Nishina, A., Kubota, K., Kameoka, H. and Osawa, T. (1991). Antioxidizing Component, Musizin, in Rumex Japonicus Houtt. Journal of the American Oil Chemists Society, 68, 735-739. Onar, H.C., Yusufoğlu, A., Türker, G. ve Yanardağ, R. (2012). Elastase, tyrosinase and lipoxygenase inhibition and antioxidant activity of an aqueous extract from Epilobium angustifolium L. leaves. Journal of Medicinal Plants Research, 6, 716-726. Partelli, S., Frulloni, L., Minniti, C., Bassi, C., Barugola, G., Onofrio, M., Stefano, C. and Falconi, M. (2012). Faecal elastase-1 is an independent predictor of survival in advanced pancreatic cancer. Digestive and Liver Disease, 44, 945-951. Robert, L. (2001). Extracellular matrix and aging: a review of mechanisms and interventions. Cosmet Toiletries Magazine, 116, 61-70. Vandivier, R. W., Fadok, V. A., Hoffmann, P. R., Bratton, D. L, Penvari, C. and Brown, K. K. (2002). Elastase-mediated phosphatidylserine receptor cleavage impairs apoptotic cell clearance in cystic fibrosis and bronchiectasis. Journal of Clinical Investigation 109, 661-70.