Moleküler Baskılanmış Polimer Temelli Kreatinin-Seçici Katı-Hal Mikrosensör

Yıl 2021, Cilt: 11 Sayı: 2, 584 - 599, 15.12.2021

Nurşen Dere Zuhal Yolcu , Murat Yolcu

https://doi.org/10.31466/kfbd.956652

Öz

Kreatinin baskılanmış polimer temelli bütünüyle katı-hal polivinilklorür (PVC)-membran potansiyometrik kreatinin-seçici mikrosensör geliştirildi. Baskılanmış polimer sentezinde; kalıp molekül, fonksiyonel monomer ve çapraz bağlayıcı olarak sırasıyla; kreatinin, metakrilik asit ve etilen glikol dimetakrilat (EGDMA) kullanıldı. Elde edilen kreatinin-baskılanmış polimerin iyonofor olarak kullanılmasıyla, PVC-membran yapısında kreatinine karşı seçici bir yanıt elde edildi. Kreatinin-seçici mikrosensörün bazı potansiyometrik performans özellikleri (doğrusal çalışma aralığı, tayin sınırı, seçicilik, eğim, cevap süresi, kullanım ömrü, pH ve sıcaklık vb.) incelendi. Hazırlanan mikrosensör, 57,2±1,2 mV (R2: 0,9979) eğimle 10-1-10-5 mol.L-1 konsantrasyon aralığında Nernst davranışı sergiledi. Geliştirilen mikrosensör elde edilen potansiyellerde önemli farklılıklar olmaksızın yaklaşık olarak altı hafta boyunca kullanıldı. Geliştirilen mikrosensörün tayin sınırı 5,0x10-6 mol.L-1 ve cevap süresi oldukça kısaydı (<15 s). Mikrosensörün pH çalışma aralığı 6,0-10,0 olarak belirlendi. Geliştirilen mikrosensör kullanılarak, sentetik numunelerde bulunan kreatinin başarıyla tayin edildi. Elde edilen potansiyometrik veriler, UV spektroskopi yöntemi ile elde edilen verilerle karşılaştırıldı ve metotların %95 güven sevisinde uyumlu olduğu sonucuna varıldı.

Anahtar Kelimeler

Kreatinin, Mikrosensör, Moleküler Baskılanmış Polimer, Potansiyometri, PVC membran

Destekleyen Kurum

Giresun Üniversitesi

Proje Numarası

FEN-BAP-C-281119-81

Teşekkür

Yazarlar, Giresun Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı'nın desteğine (proje no: FEN-BAP-C-281119-81) teşekkür eder.

Creatinine-Selective Solid-State Microsensor Based on Molecular Imprinted Polymer

Öz

A solid-state polyvinylchloride (PVC)-membrane potentiometric creatinine-selective microsensor based on a creatinine-imprinted polymer was developed. For the imprinted polymer synthesis process; creatinine, methacrylic acid and ethylene glycol dimethacrylate (EGDMA) were used as template molecule, functional monomer and crosslinker, respectively. By using the obtained creatinine-imprinted polymer as an ionophore, a selective response was obtained towards creatinine in the PVC-membrane structure. The potentiometric performance characteristics (linear operating range, detection limit, selectivity, slope, response time, lifetime, pH and temperature, etc.) of the creatinine-selective microsensor were investigated. The prepared microsensor exhibited Nernst behavior in the concentration range of 10-1-10-5 mol.L-1 with a slope of 57.2±1.2 mV (R2: 0.9979). The developed microsensor was used for approximately six weeks without significant differences in the potentials optained for creatinine. The detection limit of the developed microsensor was 5.0x10-6 mol. L-1 and the response time was rather short (<15 s). The pH working range of the microsensor was determined as 6.0-10.0. Using the developed microsensor, the creatinine determination was successfully performed in synthetic samples. The obtained potentiometric data were compared with the results obtained by UV spectroscopy method. The obtained results were in good harmony in 95% confidence level.

Anahtar Kelimeler

Creatinine, Microsensor, Molecular Imprinted Polymer, Potentiometry, PVC membrane

Proje Numarası

FEN-BAP-C-281119-81

Kaynakça

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