Endemik Onosma nana DC.’nin Kimyasal Bileşimi, Antimikrobiyal, Antioksidan ve Enzim İnhibisyon Aktivitesinin Araştırılması

Yıl 2024, Cilt: 14 Sayı: 2, 982 - 996, 18.06.2024

Kadriye Özcan Tuba Acet

https://doi.org/10.31466/kfbd.1472704

Öz

Türkçe adı “Emzik otu” olarak bilinen Onosma türleri, etnobotanik açıdan oldukça değerlidir. Bu çalışmada, Onosma nana’nın toprak üstü ve kök kısımlarının farklı çözücülerle (etanol, metanol ve etil asetat) elde edilen ekstrelerinin antioksidan, antimikrobiyal, enzim inhibisyon (α-amilaz, α-glukozidaz ve tirozidaz) ve DNA koruyucu aktivitesi ile fenolik bileşen analizinin (HPLC) yapılması amaçlanmıştır. Elde edilen sonuçlara göre, etanol ekstrelerinin diğer ekstrelere nazaran araştırılan özellikler bakımından daha etkin olduğu bulunmuştur. Etanol ekstrelerinin major fenolik bileşeni rosmarinik asit (kök: 2883.3 μg/g ekstre, toprak üstü: 11187.5 μg/g ekstre) olarak tespit edilmiştir. Sonuç olarak, bitkinin oldukça etkili antimikrobiyal aktiviteye sahip olduğu bulunmuştur (S. epidermidis ve P. vulgaris: MİK 32µg/mL). Bitkinin DNA koruyucu etkisi ve antimikrobiyal özellikleri ilk defa araştırılmıştır. Bitkinin doğal bir ajan olarak kullanılma potansiyeli olduğu söylenebilir. Bunun yanısıra, tespit edilen aktivitelerin detaylı çalışmalarla desteklenerek etki mekanizmalarının aydınlatılması ticari öneminin anlaşılması bakımından gereklidir.

Anahtar Kelimeler

Antimikrobiyal, Enzim inhibisyon, Antioksidan, Fenolik içerik, Onosma nana, HPLC

Destekleyen Kurum

Giresun Üniversitesi Bilimsel Araştırma Projeleri

Proje Numarası

FEN-BAP-A-240222-25

Teşekkür

Bu çalışma, Giresun Üniversitesi Bilimsel Araştırma Projeleri (Proje no: FEN-BAP-A-240222-25) tarafından desteklenmiştir.

Investigation of Chemical Composition, Antimicrobial, Antioxidant and Enzyme Inhibition Activity of Endemic Onosma nana DC.

Öz

Onosma species, known as “Emzik otu” in Turkish, are very valuable in terms of ethnobotany. In this study, it was aimed to analyze the antioxidant, antimicrobial, enzyme inhibition (α-amylase, α-glucosidase and tyrosidase) and DNA protective activities and phenolic compounds (HPLC) of the extracts obtained from the aboveground and root parts of Onosma nana with different solvents (ethanol, methanol and ethyl acetate). According to the results obtained, ethanol extracts were found to be more effective than other extracts in terms of the investigated properties. The major phenolic component of ethanol extracts was rosmarinic acid (root: 2883.3 μg/g extract, aboveground: 11187.5 μg/g extract). As a result, the plant was found to have highly effective antimicrobial activity (S. epidermidis and P. vulgaris: MIC 32µg/mL). The DNA protective effect and antimicrobial properties of the plant have been investigated for the first time. It can be said that the plant has the potential to be used as a natural agent. In addition, it is necessary to elucidate the mechanisms of action by supporting the detected activities with detailed studies in order to understand its commercial importance.

Anahtar Kelimeler

Antimicrobial, Enzyme inhibition, Antioxidant, Phenolic content, Onosma nana, HPLC

Proje Numarası

FEN-BAP-A-240222-25

Kaynakça

• Acet, T., Ozcan, K., and Zengin, G. (2020). An assessment of phenolic profiles, fatty acid compositions, and biological activities of two Helichrysum species: H. plicatum and H. chionophilum. Journal of Food Biochemistry, 44, e13128.
• Ahmad, B., Ali, N., Bashir, S., Choudhary, M.I., Azam, S., and Khan, I. (2009). Parasiticidal, antifungal and antibacterial activities of Onosma griffithii Vatke. African journal of Biotechnology, 8, 5084-5087.
• Binzet R., Kandemir, I., and Orcan, N. (2018). Numerical taxonomic study of the genus Onosma L. (Boraginaceae) from eastern mediterranean region in Turkey. Pakistan Journal of Botany, 50(2), 561-573.
• Brand-Williams, W., Cuvelier, M., and Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. Food Science and Technology, 28, 25-30.
• Bozkir, B., Acet, T., and Özcan, K. (2022). Investigation of the effects of different extraction methods on some biological activities of Dactylorhiza romana subsp. georgica (Klinge) Soó ex Renz & Taubenheim. South African Journal of Botany, 149, 347-354.
• Clinical and Laboratory Standards Institute (CLSI), (2017). Performance Standards for Antimicrobial Susceptibility Testing; 27th Informational Supplement. CLSI/NCCLS, 27th ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA.
• Çalhan, D.S., and Gündogan, M. (2019). Evaluation of changes in the biological activity of Onosma sericeum Willd (Boraginaceae) based on collection time and extraction solvent, and determination of its mineral and trace element composition. Journal of the Turkish Chemical Society, Section A: Chemistry, 6, 355-364.
• Elshafie H.S, Camele I., and Mohamed, A.A. (2023). A Comprehensive Review on the Biological, Agricultural and Pharmaceutical Properties of Secondary Metabolites Based-Plant Origin. International Journal of Molecular Sciences, 24(4), 3266.
• Fahmy, N., Fayez, S., Zengin, G., Selvi, S., Uba, A.I, Mollica, A., Bouyahya, A., Andriano, M., Abdelhakim, B., Ponniya, S., Nilofar, N., Lekmine, S., Claudio, F., and Eldahshan, O. (2024). Chemical exploration of different extracts from Phytolacca americana leaves and their potential utilization for global health problems: ın silico and network pharmacology validation. Journal of Biomolecular Structure and Dynamics, DOI: 10.1080/07391102.2024.2308770
• Güner, A., Aslan, S., Ekim, T., Vural, M. ve Babaç, M.T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayınları, 1. Baskı, 234-240.
• Hashemi, M.M. Marjani, M., Poursharifi, N., and Marjani, A. (2021). Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line. Journal of Basic and Clinical Physiology and Pharmacology, 33(4), 487-492.
• Jabbar, A.A. (2021). Onosma mutabilis: Phytochemical composition, antioxidant, cytotoxicity, and acute oral toxicity. Food Science Nutrition, 9, 5755-5764.
• Jaradat, N., Khasati, A., Hawi, M., Hawash, M., Shekfeh, S., Qneibi, M., Eid, A.M., Arar, M. and Qaoud M.T. (2012) Antidiabetic, antioxidant, and anti-obesity effects of phenylthio-ethyl benzoate derivatives, and molecular docking study regarding α-amylase enzyme. Scientific Reports, 12, 3108.
• Keeney, K.M., Yurist-Doutsch, S., Arrieta, M., and Finlay, B.B. (2014). Effects of Antibiotics on Human Microbiota and Subsequent Disease. Annual Review of Microbiology, 68(1), 217-235.
• Kilinç, N. (2021). Molecular Mechanisms of Possible Action of Naphthoquinones from Onosma in the Treatment and Prevention of COVID-19. Caucasian Journal of Science, 8, 173-185.
• Kirkan, B., Sarikurkcu, C., Binzet, R. and Tepe, B. (2023). Two Onosma Species (Onosma microcarpum and O. nana) as an Alternative Source of Multifunctional Agents: Biological and Phytochemical Evaluation, 13(1), 77.
• Koyuncu, O., Yaylacı, Ö.K., Kurtuluş, Ö., Sezer, O. and Öztürk, D. (2013). A new Onosma (Boraginaceae) species from Central Anatolia, Turkey. Plant Systematics and Evolution, 299, 1839–1847.
• Kundaković, T., Stanojković, T., Juranić, Z., and Kovačević, N. (2006). Cytotoxicity in vitro of naphthazarin derivatives from Onosma arenaria. Phytotherapy Research, 20(7), 602-604.
• Maldonado, E., Morales-Pison, S., Urbina, F., and Solari, A. (2023). Aging Hallmarks and the Role of Oxidative Stress, Antioxidants, 12(3), 651.
• Noor, S., Mohammad, T., Rub, M. A., Raza, A., Azum, N., Yadav, D. K., Hassan M.I., and Asiri, A.M. (2022). Biomedical features and therapeutic potential of rosmarinic acid. Archives of Pharmacal Research, 45(4), 205-228.
• Özcan, K. (2019). Artabel Gölleri (Gümüşhane) Sedimentlerinden İzole Edilen Aktinobakterilerin Antimikrobiyal Madde ve Endüstriyel Önemi Olan Enzimleri Üretme Kapasitelerinin Belirlenmesi. Journal of Anatolian Environmental and Animal Sciences, 4(2), 166-173.
• Özcan, K. (2020). Antibacterial, antioxidant and enzyme inhibition activity capacities of Doronicum macrolepis (FREYN&SINT): An endemic plant from Turkey. Saudi Pharmaceutical Journal, 28(1), 95-100.
• Özcan, T. (2010). Characterization of Onosma bracteosum Hausskn. & Bornm. and Onosma thracicum Velen. Based on Fatty Acid Compositions and α-Tocopherol Contents of the Seed Oils. European Journal of Biology, 68(2), 75-83.
• Özer, M.S., Kirkan, B., Sarikurkcu, C., Cengiz, M., Ceylan, O., Atilgan N. and Tepe, B. (2018). Onosma heterophyllum: Phenolic composition, enzyme inhibitory and antioxidant activities. Industrial Crops and Products, 111, 179–184.
• Özgen, U., Houghton, P.J., Ogundipe, Y., and Coşkun, M. (2003). Antioxidant and antimicrobial activities of Onosma argentatum and Rubia peregrina. Fitoterapia, 74, 682-685.
• Qaderi, M.M., Martel, A.B., and Strugnell, C.A. (2023). Environmental Factors Regulate Plant Secondary Metabolites. Plants. 12(3), 447.
• Safavi, F., Moridi Farimani, M., Golalipour, M., and Bayat, H. (2023). In vitro wound healing potential of cyclohexane extract of Onosma dichroantha Boiss. based on bioassay-guided fractionation. Scientific Reports, 13(1), 5018.
• Sarikurkcu, C., Kirkan, B., Ozer, M.S., Ceylan, O., Atilgan N., Cengiz, M. and Tepe, B. (2018). Chemical characterization and biological activity of Onosma gigantea extracts. Industrial Crops and Products, 115, 323–329.
• Sarikurkcu, C., Sahinler, S.S., Ceylan, O., and Tepe, B. (2020). Onosma pulchra: Phytochemical composition, antioxidant, skin-whitening and anti-diabetic activity. Industrial Crops and Products, 154, 112632.
• Sarikurkcu, C., Sahinler, S.S., Ceylan, O., and Tepe, B. (2020). Onosma ambigens: Phytochemical composition, antioxidant and enzyme inhibitory activity. Industrial Crops and Products, 154, 112651.
• Shilov, S.V., Ustenova, G.O, Kiyekbayeva, L.N., Korotetskiy, I.S., Kudashkina, N.V., Zubenko, N.V., Parenova, R.A., Jumagaziyeva, A.B., Iskakbayeva, Z.A., and Kenesheva, S.T. (2022). Component Composition and Biological Activity of Various Extracts of Onosma gmelinii (Boraginaceae), International Journal of Biomaterials, 4427804.
• Sun, H., Saeedi P., Karuranga, S., Pinkepank, M., Ogurtsova, K., Duncan, B.B., Stein, C., Basit, A., Chan, J.C.N., Mbanya, J.C., Pavkov, M.E., Ramachandaran, A., Wild, S.H., James, S., Herman, W.H., Zhang, P., Bommer, C., Kuo, S., Boyko, E.J. and Magliano, D.J. (2022). IDF Diabetes Atlas: Global, regional and country-level diabetes prevalence estimates for 2021 and projections for 2045. Diabetes Research and Clinical Practice, 183, 109119.
• Stanković, J.S.K., Ceylan, R., Zengin, G., Matić, S., Jurić, T., Diuzheva, A., Jeko, J., and Cziáky, Z. (2020). Abdurrahman Aktumsek, Multiple biological activities of two Onosma species (O. sericea and O. stenoloba) and HPLC-MS/MS characterization of their phytochemical composition. Industrial Crops and Products, 144, 112053.
• Tosun, A., Küpeli Akkol, E., Bahadır, Ö., Yesilada E. (2008). Evaluation of anti-inflammatory and antinociceptive activities of some Onosma L. species growing in Turkey. Journal of Ethnopharmacology, 120, 378–381
• Ünal, N., Kahraman, O., Dögen, Aç, Binzet, R. (2023). Investigation of the Chemical Composition, Antimicrobial, and Antioxidant Activity of Endemic Onosma halophila Boiss. & Heldr. Current Microbiology, 20,80(8),247.
• Wang, Z., Li, F., Aga, E.B., Liang, X., He, C., Yin, L., and Lv, C. (2024). Pterocephalodes hookeri-Onosma hookeri’decoction protects against LPS-induced pulmonary inflammation via inhibiting TLR4/NF-κB signaling pathway. Journal of Ethnopharmacology, 318, 116918.
• Wang, L., Pan, X., Jiang, L., Chu, Y., Gao, S., Jiang, X., Zhang, Y., Chen, Y., Luo, S. and Peng, C. (2022). The biological activity mechanism of chlorogenic acid and its applications in food industry: A review. Frontiers in Nutrition, 9, 943911. Zeljkovıć, S.C, Sahinler, S. S., Sarikurkcu, C., Kirkan, B., Binzet, R., and Tarkowski, P. (2023). Exploring the Pharmacological Potential of Onosma riedliana: Phenolic Compounds and Their Biological Activities. Plant Foods for Human Nutrition, 1-7.