Chlorolilaea pamvotia (Lortou&Gkelis) Lortou & Gkelis 2023: Türkiye Tatlısu Yeşil Alglerinin Yeni Bir Kaydı
Yıl 2025,
Cilt: 15 Sayı: 1, 16 - 23, 15.03.2025
Sibel Altürk Karaca
,
Elif Soylu
Öz
Yeşil algler (Chlorophyta), çoğunluğu tatlı sularda, çok az bir kısmı tuzlu sularda bulunan fotosentetik organizmalardır. Bu grup, çeşitlilik açısından zengin bir yelpazeye sahiptir. Bundan dolayı, farklı türlerin morfolojik benzerlikleri ve küçük boyutları nedeniyle doğru kimliklendirme için moleküler karakterizasyon gereklidir. Son yıllarda giderek daha yaygın hale gelen moleküler teknikler, morfolojik olarak ayırt edilemeyen veya nadir görülen türlerin kimliklendirilmesine olanak tanır. Bu çalışmada, Batlama Deresi’nden izole edilen yeşil alglerin morfolojik kimliklendirmesinin ardından, 18S rRNA moleküler dizileme yöntemi uygulanmıştır. Elde edilen sonuçlar, izole edilen türün Chlorolilaea pamvotia (Lortou & Gkelis) Lortu & Gkelis, 2023 olduğunu ve bu türün Türkiye'nin tatlı su alg florasında ilk kez kaydedildiğini ortaya koymuştur.
Etik Beyan
The authors declare that this study complies with Research and Publication Ethics.
Teşekkür
The authors would like to thank BM Software Consulting and Laboratory Systems Limited Company for their contribution to this research.
Kaynakça
- Agger, S. A., Lopez-Gallego, F., Hoye, T. R., & Schmidt-Dannert, C. (2008). Identification of sesquiterpene synthases from Nostoc punctiforme PCC 73102 and Nostoc sp. strain PCC7120. Journal of Bacteriology, 190(12), 6084-6096.
- Bermejo, E., Filali, R., & Taidi, B. (2021). Microalgae culture quality indicators: A review. Critical Reviews in Biotechnology, 41(4), 457-473. https://doi.org/10.1080/07388551.2020.1854672
- Bortolus, A. (2008). Error cascades in the biological sciences: The unwanted consequences of using bad taxonomy in ecology. Ambio, 37(2), 114-118.
- Demiriz, T. (2008). Bazı Alg Türlerinin Antibakteriyel Etkileri. Yüksek Lisans Tezi, Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
- Dorigo, M., & Blom, C. (2005). Colony optimization theory: A survey. Theoretical Computer Science, 344, 243-278.
- Fawley, M. W., Fawley, K. P., & Buchheim, M. A. (2004). Molecular diversity among communities of freshwater microchlorophytes. Microbial Ecology, 48, 489-499.
- Forney, S. D. (2004). Introduction to entertainment media. Retrieved from https://onlinelibrary.wiley.com/doi/full/10.1002/ss.137
- Friedl, T., & O’Kelly, C. J. (2002). Phylogenetic relationship of green algae assigned to the genus Planophila (Chlorophyta): Evidence from 18S rDNA sequence data and ultrastructure. European Journal of Phycology, 37 (3), 373-384.
- Graham, L. E., & Wilcox, L. W. (2000). Algae. Prentice Hall, Upper Saddle River.
- Guiry, M. D., & Guiry, G. M. (2023). AlgaeBase. World-wide electronic publication. National University of Ireland. Retrieved from http://www.algaebase.org
- Khaw, Y. S., Khong, N. M. H., Shaharuddin, N. A., & Fatimah, M. Y. (2020). A simple 18S rDNA approach for the identification of cultured eukaryotic microalgae with an emphasis on primers. Journal of Microbiological Methods, 172, 105890.
- Krienitz, L., & Bock, C. (2012). Present state of the systematics of planktonic coccoid green algae of inland waters. Hydrobiologia, 698, 295-326.
- Krienitz, L., Bock, C., Dadheech, P. K., & Pröschold, T. (2011). Taxonomic reassessment of the genus Mychonastes (Chlorophyceae, Chlorophyta) including the description of eight new species. Phycologia, 50, 89-106.
- Lee, X. J., Ong, H. C., Gan, Y. Y., Chen, W. H., & Mahlia, T. M. I. (2020). State of art review on conventional and advanced pyrolysis of macroalgae and microalgae for biochar, bio-oil, and bio-syngas production. Energy Conversion and Management, 210, 112707.
- Leliaert, F., Verbruggen, H., Vanormelingen, P., Steen, F., López-Bautista, J. M., Zuccarello, G. C., & De Clerck, O. (2014). DNA-based species delimitation in algae. European Journal of Phycology, 49, 179-196.
- Lortou, U., Panteris, E., & Gkelis, S. (2022). Uncovering new diversity of photosynthetic microorganisms from the Mediterranean region. Microorganisms, 10(8), 1571.
- Maraşlıoğlu, F., & Gönülol, A. (2023). Turkish algae electronic publication, Çorum, Turkey. Retrieved from http://turkiyealgleri.hitit.edu.tr
- Moon-van der Staay, S. Y., De Wachter, R., & Vaulot, D. (2001). Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature, 409, 607-610.
- Ometto, F., Quiroga, G., Pšenička, P., Whitton, R., Jefferson, B., & Villa, R. (2014). Impacts of microalgae pre-treatments for improved anaerobic digestion: Thermal treatment, thermal hydrolysis, ultrasound, and enzymatic hydrolysis. Water Research, 65, 350-361. https://doi.org/10.1016/j.watres.2014.07.040
- Rowan, R., & Powers, A. D. (1991). Molecular genetic identification of symbiotic dinoflagellates (Zooxanthellae). Marine Ecology Progress Series, 71, 65-73.
- Smith, A. G., Tredici, M. R., Boussiba, S., Verdelho, V., Cadoret, J. P., Davey, M. P., Huete-Ortega, M., Acien, F. G., Schmid-Staiger, U., Rodriguez, H., Benemann, J., Leu, S., Rodolfi, L., Biondi, N., & Meinerz, L. (2021). What are algae? EABA Websites, Retrieved from https://www.what-are-algae.com/
- Soylu, E. N., & Gönülol, A. (2012). Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. Turkish Journal of Biology, 36, 247-254. https://doi.org/10.3906/biy-1001-19
- Tringe, S. G. (2008). A renaissance for the pioneering 16S rRNA gene. Current Opinion in Microbiology, 11(5), 442-446.
Chlorolilaea pamvotia (Lortou&Gkelis) Lortou & Gkelis 2023: A new record of freshwater green algae (Chlorophyta) in Türkiye
Yıl 2025,
Cilt: 15 Sayı: 1, 16 - 23, 15.03.2025
Sibel Altürk Karaca
,
Elif Soylu
Öz
Green algae (Chlorophyta) are photosynthetic organisms, the majority of which inhabit freshwater environments, with only a small proportion found in saltwater. This group represents a diverse range of species. Therefore, due to the morphological similarities and small size of different species, molecular characterization is essential for accurate identification. Molecular techniques, which have become increasingly widespread in recent years, allow for the identification of species that are morphologically indistinguishable or rare. In this study, 18S rRNA molecular sequencing was performed following the morphological identification of green algae isolated from Batlama Stream. The results confirmed that the isolated species was Chlorolilaea pamvotia (Lortou & Gkelis) Lortu & Gkelis, 2023. This marks the first record of this species in the freshwater algal flora of Türkiye.
Etik Beyan
The authors declare that this study complies with Research and Publication Ethics.
Teşekkür
The authors would like to thank BM Software Consulting and Laboratory Systems Limited Company for their contribution to this research.
Kaynakça
- Agger, S. A., Lopez-Gallego, F., Hoye, T. R., & Schmidt-Dannert, C. (2008). Identification of sesquiterpene synthases from Nostoc punctiforme PCC 73102 and Nostoc sp. strain PCC7120. Journal of Bacteriology, 190(12), 6084-6096.
- Bermejo, E., Filali, R., & Taidi, B. (2021). Microalgae culture quality indicators: A review. Critical Reviews in Biotechnology, 41(4), 457-473. https://doi.org/10.1080/07388551.2020.1854672
- Bortolus, A. (2008). Error cascades in the biological sciences: The unwanted consequences of using bad taxonomy in ecology. Ambio, 37(2), 114-118.
- Demiriz, T. (2008). Bazı Alg Türlerinin Antibakteriyel Etkileri. Yüksek Lisans Tezi, Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
- Dorigo, M., & Blom, C. (2005). Colony optimization theory: A survey. Theoretical Computer Science, 344, 243-278.
- Fawley, M. W., Fawley, K. P., & Buchheim, M. A. (2004). Molecular diversity among communities of freshwater microchlorophytes. Microbial Ecology, 48, 489-499.
- Forney, S. D. (2004). Introduction to entertainment media. Retrieved from https://onlinelibrary.wiley.com/doi/full/10.1002/ss.137
- Friedl, T., & O’Kelly, C. J. (2002). Phylogenetic relationship of green algae assigned to the genus Planophila (Chlorophyta): Evidence from 18S rDNA sequence data and ultrastructure. European Journal of Phycology, 37 (3), 373-384.
- Graham, L. E., & Wilcox, L. W. (2000). Algae. Prentice Hall, Upper Saddle River.
- Guiry, M. D., & Guiry, G. M. (2023). AlgaeBase. World-wide electronic publication. National University of Ireland. Retrieved from http://www.algaebase.org
- Khaw, Y. S., Khong, N. M. H., Shaharuddin, N. A., & Fatimah, M. Y. (2020). A simple 18S rDNA approach for the identification of cultured eukaryotic microalgae with an emphasis on primers. Journal of Microbiological Methods, 172, 105890.
- Krienitz, L., & Bock, C. (2012). Present state of the systematics of planktonic coccoid green algae of inland waters. Hydrobiologia, 698, 295-326.
- Krienitz, L., Bock, C., Dadheech, P. K., & Pröschold, T. (2011). Taxonomic reassessment of the genus Mychonastes (Chlorophyceae, Chlorophyta) including the description of eight new species. Phycologia, 50, 89-106.
- Lee, X. J., Ong, H. C., Gan, Y. Y., Chen, W. H., & Mahlia, T. M. I. (2020). State of art review on conventional and advanced pyrolysis of macroalgae and microalgae for biochar, bio-oil, and bio-syngas production. Energy Conversion and Management, 210, 112707.
- Leliaert, F., Verbruggen, H., Vanormelingen, P., Steen, F., López-Bautista, J. M., Zuccarello, G. C., & De Clerck, O. (2014). DNA-based species delimitation in algae. European Journal of Phycology, 49, 179-196.
- Lortou, U., Panteris, E., & Gkelis, S. (2022). Uncovering new diversity of photosynthetic microorganisms from the Mediterranean region. Microorganisms, 10(8), 1571.
- Maraşlıoğlu, F., & Gönülol, A. (2023). Turkish algae electronic publication, Çorum, Turkey. Retrieved from http://turkiyealgleri.hitit.edu.tr
- Moon-van der Staay, S. Y., De Wachter, R., & Vaulot, D. (2001). Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature, 409, 607-610.
- Ometto, F., Quiroga, G., Pšenička, P., Whitton, R., Jefferson, B., & Villa, R. (2014). Impacts of microalgae pre-treatments for improved anaerobic digestion: Thermal treatment, thermal hydrolysis, ultrasound, and enzymatic hydrolysis. Water Research, 65, 350-361. https://doi.org/10.1016/j.watres.2014.07.040
- Rowan, R., & Powers, A. D. (1991). Molecular genetic identification of symbiotic dinoflagellates (Zooxanthellae). Marine Ecology Progress Series, 71, 65-73.
- Smith, A. G., Tredici, M. R., Boussiba, S., Verdelho, V., Cadoret, J. P., Davey, M. P., Huete-Ortega, M., Acien, F. G., Schmid-Staiger, U., Rodriguez, H., Benemann, J., Leu, S., Rodolfi, L., Biondi, N., & Meinerz, L. (2021). What are algae? EABA Websites, Retrieved from https://www.what-are-algae.com/
- Soylu, E. N., & Gönülol, A. (2012). Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. Turkish Journal of Biology, 36, 247-254. https://doi.org/10.3906/biy-1001-19
- Tringe, S. G. (2008). A renaissance for the pioneering 16S rRNA gene. Current Opinion in Microbiology, 11(5), 442-446.