Assessing the Proliferative Impact of Alginate-Gelatin Composites on Mesenchymal Stromal Cells

Yıl 2025, Cilt: 15 Sayı: 1, 119 - 132, 15.03.2025

Mesude Biçer

https://doi.org/10.31466/kfbd.1496088

Öz

Traditional conducted on flat two-dimensional (2D) culture do not closely mirror the physiological and pathophysiological features of the in vivo environment including cell migration, soluble factor gradient and cell-matrix interactions. Three-dimensional (3D) cell culture overcomes these drawbacks by using 3D biomaterials, such as alginate hydrogels, to promote cell migration, vary biomaterial stiffness or permit cell-matrix interactions. In this study, it was aimed to propose a novel 3D platform including alginate-gelatin composites to address the need for tissue engineering techniques that support mesenchymal stromal cell viability and differentiation potential. In the first part, the absorbance spectra of different hydrogel-based biomaterials were examined using UV-visible light. After finding the best performing hydrogel, the work focused on cell proliferation using XTT viability assay and Live/Dead cytotoxicity assay. The cell viability of mesenchymal stromal cells in the best hydrogel biomaterial was compared to other scaffolds including cellulose, alginate-gelatin and only alginate. Alginate-gelatin hydrogel increased MSC viability, in comparison with other scaffolds such as alginate and cellulose. This increase also was significant compared to the cells grown in 2D culture. The findings of this study are consistent with the data of other studies in the literature. Thus, alginate-gelatin composites could be a promising candidate in tissue engineering to improve cell proliferation.

Anahtar Kelimeler

Mesenchymal stromal cells, Alginate-gelatin composite, Cell viability, Tissue engineering, 3D cell culture

Aljinat-jelatin Kompozitlerin Mezenkimal Kök Hücreleri Üzerindeki Proliferatif Etkisinin Değerlendirilmesi

Öz

İki boyutlu (2D) kültür üzerinde yapılan geleneksel çalışmalar, hücre göçü, çözünür faktör gradyanı ve hücre-matris etkileşimleri dahil olmak üzere in vivo ortamın fizyolojik ve patofizyolojik özelliklerini yakından yansıttmaz. 3-boyutlu (3D) hücre kültürü, hücre göçünü teşvik etmek, biyomateryal sertligini degiştirmek veya hücre-matris etkileşimlerine izin vermek için aljinat hidrojelleri gibi 3D biyomateryalleri kullanarak bu dezavantajların üstesinden gelmektedir. Bu çalışmada, mezenkimal kok hücre canlılığını ve farklılaşma potansiyelini destekleyen doku mühendisliği tekniklerine olan ihtiyacı karşılamak için aljinat-jelatin kompozitleri içeren yeni bir 3-boyutlu platformun önerilmesi amaçlanmıştır. İlk bölümde farklı hidrojel bazlı biyomateryallerin absorbans spektrumları görünür ışık kullanılarak incelenmiştir. En iyi performansı gösteren hidrojeli bulduktan sonra çalışma, XTT canlılık testi ve canlı/ölü sitotoksisite testi kullanılarak hücre çoğalmasına odaklanılmıştır. Hücre canlılığı aljinat-jelatin, aljinat ve selüloz gibi iskelelerle karşılaştırıldığında, aljinat-jelatin hidrojelin mezenkimal kök hücrelerinin canlılığını arttırdığı tespit edildi. Bu artış, 2D kültürde büyütülen hücrelerle karşılaştırıldığında da aynı şekilde gözlendi. Bu çalışmadan elde edilen bulgular, literatürdeki diğer çalışmaların verileriyle tutarlıdır. Bu nedenle, aljinat-jelatin kompozitleri doku mühendisliğinde hücre proliferasyonunu iyileştirmek için umut verici bir aday olabilir.

Anahtar Kelimeler

Mezenkimal kök hücreler, Aljinat-jelatin kompozit, Hücre canlılığı, Doku mühendisliği, 3D hücre kültürü

Kaynakça

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