Gökkuşağı Alabalığı Dokularında Myxobolus Cerebralis Üzerine Detaylı Bir Histopatolojik Araştırma

Yıl 2025, Cilt: 15 Sayı: 1, 182 - 190, 15.03.2025

Ezgi Dinçtürk , Ttansel Tanrıkul

https://doi.org/10.31466/kfbd.1521196

Öz

Dönme hastalığı etkeni olan Myxobolus cerebralis, salmonid balıklarını olumsuz etkileyerek önemli ekonomik kayıplara neden olmaktadır. Bu çalışmada, dönme hastalığının klinik belirtilerini sergileyen enfekte gökkuşağı alabalığı (Oncorhynchus mykiss) örneklenmiş ve kapsamlı bir histopatolojik incelemeye tabi tutulmuştur. Sonuçlar beyinde, dalakta ve solungaç dokularında M. cerebralis sporlarının iltihaplanma, nekroz ve kist oluşumu gibi dikkate değer patolojik değişikliklerle birlikte olduğunu göstermektedir. Çalışma, M.cerebralis enfeksiyonunun seyrini detaylandırarak, konakçıdaki önemli doku hasarını ve bağışıklık tepkilerini ortaya koymaktadır. Sonuçlar, patojenin balık sağlığı üzerindeki etkilerini anlamak ve konakçı doku tepkisine ilişkin veri sağlamak adına histopatolojik yöntemlerle tanımlamanın önemini göstermektedir.

Anahtar Kelimeler

Myxozoa, Dönme hastalığı, Parazit, Histopatoloji

A Detailed Histopathological Research on Myxobolus cerebralis in Rainbow Trout Tissues

Öz

The causative agent of whirling disease, Myxobolus cerebralis, has a negative impact on salmonid fish and causes significant economic losses. Infected specimens of rainbow trout (Oncorhynchus mykiss) exhibiting clinical signs of whirling disease were sampled and subjected to a thorough histopathological examination in this study. The results showed M. cerebralis spores in brain, spleen, and gill tissues, with notable pathological changes such as inflammation, necrosis, and cyst formation. The study details the progression of M.cerebralis infections, highlighting significant tissue damage and immune responses in the host. The results demonstrate the importance of identifying the pathogen with histopathological methods to understand the impacts on fish health and provide insight into the host tissue response.

Anahtar Kelimeler

Myxozoa, Whirling disease, Parasite, Histopathology

Kaynakça

• Akram, N., El-Matbouli, M., and Saleh, M. (2023). The immune response to the myxozoan parasite Myxobolus cerebralis in salmonids: A review on whirling disease. International Journal of Molecular Sciences, 24(24), 17392.
• Antonio, D. B., El-Matbouli, M., and Hedrick, R. P. (1999). Detection of early developmental stages of Myxobolus cerebralis in fish and tubificid oligochaete hosts by in situ hybridization. Parasitology Research, 85, 942-944.
• Antonio, D. B., Andree, K., McDowell, T. S., and Hedrick, R. P. (1998). Detection of Myxobolus cerebralis in rainbow trout and oligochaete tissues by using a nonradioactive in situ hybridization (ISH) protocol. Journal of Aquatic Animal Health, 10(4), 338-347.
• Arsan, E. L., Atkinson, S. D., Hallett, S. L., Meyers, T., and Bartholomew, J. L. (2007). Expanded geographical distribution of Myxobolus cerebralis: first detections from Alaska. Journal of Fish Diseases, 30(8), 483-491.
• Baldwin, T. J., Vincent, E. R., Silflow, R. M., and Stanek, D. (2000). Myxobolus cerebralis infection in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta) exposed under natural stream conditions. Journal of Veterinary Diagnostic Investigation, 12(4), 312-321.
• Blazer, V. S., Densmore, C. L., Schill, W. B., Cartwright, D. D., and Page, S. J. (2004). Comparative susceptibility of Atlantic salmon, lake trout and rainbow trout to Myxobolus cerebralis in controlled laboratory exposures. Diseases of Aquatic Organisms, 58(1), 27-34.
• Dar, S. A., Kaur, H., and Chishti, M. Z. (2017). First record of myxozoan parasites from fresh water fishes of Jammu and Kashmir and their pathogenecity. Microbial pathogenesis, 105, 138-144.
• El-Matbouli, M., Hoffmann, R.W., Schoel, H. McDowell, T.S., and Hedrick, R.P. (1999). Whirling disease:host specificity and interaction between the actinosporean stage of Myxobolus cerebralis and rainbow trout Oncorhynchus mykiss. Diseases of Aquatic Organisms, 35, 1–12.
• El-Matbouli, M. and Hoffmann, R. (1998). Light and electron microscopic studies on the chronological development of Myxobolus cerebralis to the actinosporean stage in Tubifex tubifex. International Journal for Parasitology, 28, 195–217.
• El-Matbouli, M., Hoffmann, R.W., and Mandok, C. (1995). Light and electron microscopic observations on the route of the triactinomyxon- sporoplasm of Myxobolus cerebralis from epidermis into rainbow trout cartilage. Journal of Fish Biology, 46, 919–935.
• El-Matbouli, M., and Hoffmann, R. (1989). Experimental transmission of two Myxobolus spp. developing bisporogeny via tubificid worms. Parasitology Research, 75(6), 461-464.
• Feist, S. W., and Longshaw, M. (2006). Phylum Myxozoa. In P. T. K. Woo (Ed.), Fish Diseases and Disorders (Vol. 1, pp. 230-296). CAB International.
• Gbankoto, A., Pampoulie, C., Marques, A., Sakiti, G. N., and Dramane, K. L. (2003). Infection patterns of Myxobolus heterospora in two tilapia species (Teleostei: Cichlidae) and its potential effects. Diseases of aquatic organisms, 55(2), 125-131.
• Gilbert, M. A., and Granath Jr, W. O. (2001). Persistent infection of Myxobolus cerebralis, the causative agent of salmonid whirling disease, in Tubifex tubifex. Journal of Parasitology, 87(1), 101-107.
• Gupta, A., and Kaur, H. (2017). A new pathogen, Myxobolus holzerae (Myxosporea: Myxozoa) causing severe gill disease in an Indian major carp Labeo rohita in a cold water wetland, Punjab (India). Microbial Pathogenesis, 111, 244-251.
• Halliday, M. M. 1(976) The biology of Myxosoma cerebralis, the causative agent of whirling disease of salmonids. Journal of Fish Biology, 9, 339–357.
• Hedrick, R. P., McDowell, T. S., Gay, M., Marty, G. D., Georgiadis, M. P., and MacConnell, E. (1999). Comparative susceptibility of rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta to Myxobolus cerebralis, the cause of salmonid whirling disease. Diseases of Aquatic Organisms, 37(3), 173-183.
• Hedrick, R. P., Adkison, M. A., El‐Matbouli, M., and MacConnell, E. (1998). Whirling disease: re‐emergence among wild trout. Immunological Reviews, 166(1), 365-376.
• Hoffman, G. L. (1990). Myxobolus cerebralis, a worldwide cause of salmonid whirling disease. Journal of Aquatic Animal Health, 2(1), 30-37.
• Humason, G.L. (1972) Animal Tissue Techniques. 3rd edn. San Francisco: W. H. Freeman and Company.
• Karagouni, E., Athanassopoulou, F., Lytra, A., Komis, C., and Dotsika, E. (2005). Antiparasitic and immunomodulatory effect of innovative treatments against Myxobolus sp. infection in Diplodus puntazzo. Veterinary Parasitology, 134(3-4), 215-228.
• Kelley, G. O., Zagmutt-Vergara, F. J., Leutenegger, C. M., Myklebust, K. A., Adkison, M. A., McDowell, T. S., ... and Hedrick, R. P. (2004a). Evaluation of five diagnostic methods for the detection and quantification of Myxobolus cerebralis. Journal of Veterinary Diagnostic Investigation, 16(3), 202-211.
• Kelley, G. O., Zagmutt-Vergara, F. J., Leutenegger, C. M., Adkison, M. A., Baxa, D. V., and Hedrick, R. P. (2004b). Identification of a serine protease gene expressed by Myxobolus cerebralis during development in rainbow trout Oncorhynchus mykiss. Diseases of Aquatic Organisms, 59(3), 235-248.
• Kim, W. S., Kim, J. H., Jang, M. S., Jung, S. J., and Oh, M. J. (2013). Infection of wild mullet (Mugil cephalus) with Myxobolus episquamalis in Korea. Parasitology Research, 112, 447-451.
• Liu, Y., Whipps, C. M., Gu, Z. M., Huang, M. J., He, C., Yang, H. L., and Molnár, K. (2013). Myxobolus musseliusae (Myxozoa: Myxobolidae) from the gills of common carp Cyprinus carpio and revision of Myxobolus dispar recorded in China. Parasitology Research, 112, 289-296.
• Lom, J., and Dyková, I. (2006). Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitologica, 53(1), 1-36.
• Lom, J., and Dyková, I. (1994). Protozoan Parasites of Fishes, Elsevier Science Publishers B.V., Amsterdam, the Netherlands, 1994, pp. 159e235.
• Lom, J., and G. L. Hoffman. (1970). Redescription of the spores of Myxobolus cerebralis. Journal of Parasitology, 56, 213.
• Molnár, K., Eszterbauer, E., Marton, S., Székely, C., and Eiras, J. C. (2012). Comparison of the Myxobolus fauna of common barbel from Hungary and Iberian barbel from Portugal. Diseases of Aquatic organisms, 100(3), 231-248.
• Molnár, K., Marton, S., Székely, C., and Eszterbauer, E. (2010). Differentiation of Myxobolus spp.(Myxozoa: Myxobolidae) infecting roach (Rutilus rutilus) in Hungary. Parasitology Research, 107, 1137-1150.
• O'Grodnick, J.J. (1979). Susceptibility of various salmonids to whirling disease (Myxosoma cerebralis). Transactions of the American Fisheries Society, 108(2), 187-190.
• Roberts, R. J., and Shepherd, C. J. (1974). Handbook of trout and salmon diseases (pp. 168-pp).
• Rose, J. D., Marrs, G. S., Lewis, C., and Schisler, G. (2000). Whirling disease behavior and its relation to pathology of brain stem and spinal cord in rainbow trout. Journal of Aquatic Animal Health, 12(2), 107-118.
• Thoesen, J. C., editor. (1994). Suggested procedures for the detection and identification of certain finfish and shellfish pathogens, 4th edition. American Fisheries Society, Fish Health Section, Bethesda, Maryland.
• Zhang, J. Y., Yokoyama, H., Wang, J. G., Li, A. H., Gong, X. N., Ryu‐Hasegawa, A., ... and Ogawa, K. (2010). Utilization of tissue habitats by Myxobolus wulii Landsberg & Lom, 1991 in different carp hosts and disease resistance in allogynogenetic gibel carp: redescription of M. wulii from China and Japan. Journal of Fish Diseases, 33(1), 57-68.