Karaman İli Yöresi Bitkilerinden Arum Cinsine Ait Türlerinden Arum dioscoridis’in İskelet Kası Mekanik Aktivitesi Üzerindeki Yarı Maksimum Etkin Konsantrasyon (EC50) Değerlerinin Belirlenmesi (Faz 0 – Preklinik Çalışma)

Öz

Arum dioscoridis sadece Anadolu'da değil dünyanın çeşitli yerlerinde fitoterapötik olarak kullanılan bu bitkilerden biridir. Bu bitkinin kas mekanik aktivitesi üzerine etkileri konusunda literatürde herhangi bir çalışma bulunmamaktadır. Bu pilot çalışmada, Arum dioscoridis'in iskelet kasının kasılma aktivitesi üzerindeki yarı maksimum etkili konsantrasyon (EC50) değerleri belirlendi. Maksimum toksik olmayan konsantrasyon (MnTK) değerine göre farklı konsantrasyonlarda hazırlanan AD ekstraktına maruz bırakılan Wistar albino sıçan ekstansör digitorum longus kasının mekanik tepkileri 1, 10, 20, 40, 80 ve 100 Hz frekanslarında kaydedildi. Sonuç olarak, MnTK değerinin 1/16 ve 1/32 konsantrasyonlarında kontrole göre sırasıyla 20, 40, 80 Hz ve 40, 80 Hz frekanslarında mekanik tepkide artış ve daha yüksek konsantrasyon değerlerinde bir düşüş gözlemlendi.

Anahtar Kelimeler

• Arum dioscoridis
• kas mekanik aktivitesi
• EC50
• MnTK

Determination of Half Maximal Effective Concentration (EC50) Values on Skeletal Muscle Mechanic Activityof Arum dioscoridis, a Species Belonging to Arum Genus, One of the Plants of Karaman Province Region (Phase 0 – Preclinical Studies).

Öz

Arum dioscoridis (AD) is one of the plants that is used phytotherapeutically not only in Anatolia but also in various parts of the world. There is no study in the literature on the effects of this planton on muscle mechanic activity. In this pilot study, half maximal effective concentration values (EC50) of AD on contractile activity of skeletal muscle were determined. The mechanical responses of the Wistar albino rat extensor digitorumlongus muscle, which were exposed to AD extract prepared at different concentrations according to the maximum non-toxic concentration (MnTC) value, were recorded at frequencies of 1, 10, 20, 40, 80 and 100 Hz. As a result, an increase in mechanical response was observed, at 1/16 and 1/32 concentrations of the MnTC value compared to the control in the frequencies 20, 40, 80 Hz and 40, 80 Hz, respectively, and a decrease was observed at higher concentration values.

Anahtar Kelimeler

• Arum dioscoridis
• muscle mechanical activity
• EC50
• MnTC

Kaynakça

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