Galanthus elwesii Hook.f.’nin Farklı Ekstraktlarının Serbest Radikal Yakalama ve Metal Şelatlama Aktivitelerinin Tespit Edilmesi

Öz

İnsan vücudunu oksidatif strese karşı koruyan antioksidanlar içerisinde doğal bileşikler önemli rol oynamaktadır. Birçok bitki taşıdıkları bileşikler aracılığı ile yüksek seviyede antioksidan kapasiteye sahip olabilir. Bu bilgiler doğrultusunda, mevcut çalışmada, Galanthus elwesii Hook.f.’nin antioksidan özelliklerini değerlendirmeyi amaçladık. Bu kapsamda, bu bitkinin soğan kısmından elde ettiğimiz metanol ve su ekstraktlarının farklı konsantrasyonlarının (12,5-400 mg/L) 1,1-difenil-2-pikrilhidrazil (DPPH) yakalama ve metal şelatlama aktivitelerini test ettik. Her iki ekstrakt için de konsantrasyona bağlı aktivite artışı görülmüştür. 400 mg/L konsantrasyonlu uygulamaların gösterdiği DPPH yakalama (%87,10) ve metal şelatlama (%72,99) aktiviteleri ön plana çıkmıştır. Ayrıca bu verilerin diğer uygulamaların ortaya çıkardığı aktivite oranlarından önemli derecede (p < 0,05) yüksek olduğu belirlenmiştir. IC50 değerleri göz önüne alındığında, DPPH yakalama ve metel şelatlama aktiviteleri için düşük değerler (sırasıyla, 110,88 mg/L ve 132,40 mg/L) su ekstraktlarında tespit edilmiştir. Sonuç olarak, antioksidanlar bakımından zengin bir kaynak olabilme potansiyeline sahip olan bitkiler içerisinde G. elwesii’nin önemi bu çalışma ile ortaya çıkarılmıştır.

Anahtar Kelimeler

• Antioksidan
• bitkisel ürün
• doğal bileşik

Determination of Free Radical Scavenging and Metal Chelating Activities of Different Extracts of Galanthus elwesii Hook.f.

Abstract

Natural compounds play an important role among antioxidants that protect the human body against oxidative stress. Many plants may have high levels of antioxidant capacity through the compounds they contain. In line with this information, in the current study, we aimed to evaluate the antioxidant properties of Galanthus elwesii Hook.f. In this context, we tested the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and metal chelating activities of different concentrations (12.5-400 mg/L) of methanol and water extracts obtained from the bulb part of this plant. A concentration-dependent increase in activity was observed for both extracts. DPPH scavenging (87.10%) and metal chelating (72.99%) activities demonstrated by 400 mg/L concentration applications came to the fore. In addition, it was determined that these data were significantly (p < 0.05) higher than the activity rates revealed by other applications. Considering the IC50 values, low values for DPPH scavenging and metal chelating activities (110.88 mg/L and 132.40 mg/L, respectively) were detected in water extracts. As a result, the importance of G. elwesii among plants that have the potential to be a rich source of antioxidants was revealed in this study.

Keywords

• Antioxidant
• herbal product
• natural compound

Kaynakça

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