Sisplatinin Neden Olduğu Hepatotoksisite Üzerine β-glukanın Koruyucu Rolünün Histopatolojik Olarak Değerlendirilmesi

Öz

Sisplatin pek çok kanserin tedavisinde sıklıkla kullanılan kemoterapötik bir ajandır. En önemli doz sınırlayıcı yan etkisi hepatotoksisitedir. Bu çalışmada, sisplatin tedavisinin karaciğerde neden olduğu histolojik hasar üzerinde antioksidan β-glukanın etkilerinin araştırılması amaçlandı. Wistar ratlar sacrifiye edilecek zamana göre rastgele dört gruba ayrıldılar. Bu gruplar 1. gün, 2. gün, 7. ve 14. gün (n=20 her grupta 20 rat) olarak belirlendi. Her grupta kendi içinde Kontrol, Sisplatin (10 mg/kg), β-glukan (100 mg/kg) ve Sisplatin+β-glukan (n=5 her grupta) olmak üzere dört alt gruba ayrıldı. Ratlar son enjeksiyondan sonraki 1. gün, 2. gün, 7. gün ve 14. günde sakrifiye edildi. Histolojik prosedürün ardından karaciğer kesitleri ışık mikroskobik incelendi. Sisplatinin farklı günlerde oluşturduğu histolojik hasar sinüzoidal konjesyon, hidropik dejenerasyon, hepatik kordlarda düzensizlik ve karaciğerde mononükleer hücre infiltrasyonu olarak değerlendirildi. Sisplatin ile birlikte β-glukan uygulandığında farklı gün gruplarında sisplatinin karaciğerde neden olduğu hücresel hasarın önemli ölçüde azaldığı belirlendi. Işık mikroskobik incelemede antioksidan β-glukanın serbest radikal temizleyici etkisi ile sisplatinin neden olduğu hepatotoksisiteye karşı koruma sağladığı görüldü. Sonuç olarak β-glukan, sisplatinin karaciğer üzerindeki toksisitesini azaltarak hastaların yaşam kalitesini arttırabilir.

Anahtar Kelimeler

• Sisplatin
• β-glukan
• karaciğer
• histopatoloji

Histological Evaluation of the Protective Role of β-glucan Against Cisplatin-Induced Hepatotoxicity

Abstract

Cisplatin is a commonly used chemotherapeutic agent in the treatment of many cancers. The most important dose-limiting side effect is hepatotoxicity. Some studies have shown that antioxidant treatment with cisplatin reduces the toxic effect. In the present study, we were aimed to investigate the protective effects of antioxidant β-glucan on histological injury caused by cisplatin treatment in the liver. Wistar rats were randomly divided into three groups according to time of sacrifice, 7th day and 14th day (n=20 rats each). Both groups were then divided into four sub-groups Control, Cisplatin (10 mg/kg bw), β-glucan (100 mg/kg bw) and cisplatin+β-glucan (n=5 in each group). The rats were sacrificed at the 7th day and 14th day after the last injection. The liver sections were evaluated under a light microscope after the histological procedure. Histological injury caused by cisplatin in different days were evaluated as as sinusoidal congestion, hydropic degeneration, disorganization of hepatic cords, and mononuclear cellular infiltration in liver. When β-glucan was administered with cisplatin, it was determined that cellular damage caused by cisplatin decreased considerably in the liver in the different days groups. The light microscopic examination showed that the antioxidant beta-glucan protects against hepatotoxicity caused by cisplatin with its free radical scavenging effect. In conclusion, β-glucan may improve patients' quality of life by reducing cisplatin's toxicity on the liver.

Keywords

• Cisplatin
• β-glucan
• liver
• histopathology

Kaynakça

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