Karaman İli Yöresi Bitkilerinden Arum Cinsine Ait Türlerinden Arum dioscoridis’in İskelet Kası Mekanik Aktivitesi Üzerindeki Yarı Maksimum Etkin Konsantrasyon (EC50) Değerlerinin Belirlenmesi (Faz 0 – Preklinik Çalışma)

Yıl 2021, Cilt: 3 Sayı: 2, 86 - 109, 30.12.2021

Erkan Özbay Çağatay Han Türkseven Sami Karagöz Belgin Büyükakıllı

Öz

Arum dioscoridis sadece Anadolu'da değil dünyanın çeşitli yerlerinde fitoterapötik olarak kullanılan bu bitkilerden biridir. Bu bitkinin kas mekanik aktivitesi üzerine etkileri konusunda literatürde herhangi bir çalışma bulunmamaktadır. Bu pilot çalışmada, Arum dioscoridis'in iskelet kasının kasılma aktivitesi üzerindeki yarı maksimum etkili konsantrasyon (EC50) değerleri belirlendi. Maksimum toksik olmayan konsantrasyon (MnTK) değerine göre farklı konsantrasyonlarda hazırlanan AD ekstraktına maruz bırakılan Wistar albino sıçan ekstansör digitorum longus kasının mekanik tepkileri 1, 10, 20, 40, 80 ve 100 Hz frekanslarında kaydedildi. Sonuç olarak, MnTK değerinin 1/16 ve 1/32 konsantrasyonlarında kontrole göre sırasıyla 20, 40, 80 Hz ve 40, 80 Hz frekanslarında mekanik tepkide artış ve daha yüksek konsantrasyon değerlerinde bir düşüş gözlemlendi.

Anahtar Kelimeler

Arum dioscoridis, kas mekanik aktivitesi, EC50, MnTK

Determination of Half Maximal Effective Concentration (EC50) Values on Skeletal Muscle Mechanic Activityof Arum dioscoridis, a Species Belonging to Arum Genus, One of the Plants of Karaman Province Region (Phase 0 – Preclinical Studies).

Öz

Arum dioscoridis (AD) is one of the plants that is used phytotherapeutically not only in Anatolia but also in various parts of the world. There is no study in the literature on the effects of this planton on muscle mechanic activity. In this pilot study, half maximal effective concentration values (EC50) of AD on contractile activity of skeletal muscle were determined. The mechanical responses of the Wistar albino rat extensor digitorumlongus muscle, which were exposed to AD extract prepared at different concentrations according to the maximum non-toxic concentration (MnTC) value, were recorded at frequencies of 1, 10, 20, 40, 80 and 100 Hz. As a result, an increase in mechanical response was observed, at 1/16 and 1/32 concentrations of the MnTC value compared to the control in the frequencies 20, 40, 80 Hz and 40, 80 Hz, respectively, and a decrease was observed at higher concentration values.

Anahtar Kelimeler

Arum dioscoridis, muscle mechanical activity, EC50, MnTC

Kaynakça

• Referans1 Afifi F. U., Kasabri V. & Abu-Dahab R. Medicinal plants from Jordan in the treatment of cancer: traditional uses vs. in vitro and in vivo evaluations–Part 1. Planta medica, 77(11), 1203-1209, (2011).
• Referans2 Afifi F. U., Kasabri V., Litescu S. C. & Abaza I. M. In vitro and in vivo comparison of the biological activities of two traditionally and widely used Arum species from Jordan: Arum dioscoridis Sibth & Sm. and Arum palaestinum Boiss. Natural product research, 30(16), 1777–1786, (2016).
• Referans3 Ali-Shtayeh M. S., Al-Assali A. A. & Jamous R. M. Antimicrobial activity of Palestinian medicinal plants against acne-inducing bacteria. African Journal of Microbiology Research, 7(21), 2560-2573, (2013).
• Referans4 Alı-Shtayeh M. S., Yanıv Z. & Mahajna J. Ethnobotanical surveys in the Palestinian area: A classification of the healing potential of medicinal plants. J. Ethnopharm. 73, 221–232, (2000).
• Referans5 Azab A. Arum: a plant genus with great medicinal potential. European Chemical Bulletin, 6(2), 59-68, (2017).
• Referans6 Balos M., & Akan H. Zeytinbahçe-Akarçay (Birecik, Şanlıurfa) Arasında Kalan Bölgenin Etnobotanik Özellikleri. Selçuk Üniversitesi, Fen Fakültesi, Fen Dergisi, 2 (29), 155-171, (2007).
• Referans7 Baytop T. Türkiye’de Bitkilerle Tedavi. İstanbul: Nobel Yayınları, (1999).
• Referans8 Baytop T., & Kadıoğlu D. İstanbul Bölgesi‟nin tıbbi bitkileri. In: 14. Bitkisel İlaç Hammaddeleri Toplantısı, Eskişehir, (2002).
• Referans9 Ben Ramadan L., Zwawi A., Almaghour H., Saad M., Alfalah A., Ben Amer L. & Auzi A. Egypt J. Forensic Sci. Appl. Toxicol., 12, 31, (2012).
• Referans10 Cordell G. A. & Colvard, M. D. Some thoughts on the future of ethnopharmacology. Journal of Ethnopharmacology 100, 5–14, (2005).
• Referans11 Croteau R., Kutchan T. M. & Lewis N. G. Natural products (secondary metabolites. In B.B. Buchannan, W. Gruissem and R.L. Jones (eds), Biochemistry and Molecular Biology of Plant. American Society of Plant Physiologists, 1250–1318, (2000).
• Referans12 Dewick P. M. Medicinal natural products: a biosynthetic approach. John Wiley & Sons, 0-471, (2002).
• Referans13 Doğan H. H., Karagöz S. & Duman, R. In vitro evaluation of the antiviral activity of some mushrooms from Turkey. International journal of medicinal mushrooms, 20(3), (2018).
• Referans14 Ebrahiminezhad A., Zare-Hoseinabadi A., Berenjian A. & Ghasemi Y. Green synthesis and characterization of zero-valent iron nanoparticles using stinging nettle (Urticadioica) leaf extract. Green Processing and Synthesis, 6(5), 469-475, (2017).
• Referans15 El-Desouky S. K., Kim K. H., Ryu S. Y., Eweas A. F., Gamal-Eldeen A. M. & Kim Y-K., Arch. Pharm. Res., 30, 927, (2007).
• Referans16 El-Desouky S. K., Ryu S. Y. & Kim Y. K. Tetrahedron Lett., 48, 4015, (2007).
• Referans17 Espindola A., Buerki S., Bedalov M., Kupfer P. & Alvarez N., Bot. J. Linn. Soc., 163, 14, (2010).
• Referans18 Evans W.C. Trease and Evans Pharmacognosy, 15th ed. Saunders, London, UK, (2002).
• Referans19 Feınbrun-Dothan N. Flora Palestina, 4. Text, pp. 46–47, The Israel Academy of Science and Humanity, Jerusalem, Israel, (1986).
• Referans20 Gazzaneo L., de Lucena R. & de Albuquerque U. Knowledge and use of medicinal plants by local specialists in a region of Atlantic forest in the state of Pernambuco (Northeastern Brazil). Journal of Ethnobiology and Ethnomedicine 1, 1–8, (2005).
• Referans21 Gilani A. H. & Rahman A. U. Trends in ethnopharmacology. Journal of Ethnopharmacology 100, 43–49, (2005).
• Referans22 Gunes S., Buyukakilli B., Yaman S., Turkseven C. H., Ballı E., Cimen B., Bayrak G. & Celikcan H. D. Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats. Toxicology and industrial health, 36(2), 119–131, (2020).
• Referans23 Gunther R T. The Greek Herbal of Dioscorides. London and New York: Hafner Publishing Company, (1968).
• Referans24 Heinrich M., Ankli A., Frei B., Weimann C. & Sticher O. Medicinal plants in Mexico: healers’ consensus and cultural importance. Social Science and Medicine 47, 1859–1871, (1998).
• Referans25. Janakat S. & Al‐Thnaıbat O. Antilipoperoxidative effect of three edible plants extracts: Viscum album, Arum dioscoridis and Eminium spiculatum. Journal of food quality, 31(1), 1-12, (2008).
• Referans26 Jaradat N. & Abualhasan M. Comparison of phytoconstituents, total phenol contents and free radical scavenging capacities between four Arum species from Jerusalem and Bethlehem. Pharmaceutical Sciences, 22(2), 120-125, (2016).
• Referans27 Jaradat N. A., Al-Ramahi R., Zaid A. N., Ayesh O. I. & Eid A. M. Ethnopharmacological survey of herbal remedies used for treatment of various types of cancer and their methods of preparations in the West Bank-Palestine. BMC complementary and alternative medicine, 16(1), 1-12, (2016).
• Referans28 Ivanova D., Gerova D., Chervenkov T. & Yankova T. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. Journal of ethnopharmacology, 96(1-2), 145–150, (2005).
• Referans29 Karahan F., Kulak M., Urlu E., Gözüacik H. G., Böyümez T., Şekeroğlu N. & Doganturk I. H. Total phenolic content, ferric reducing and DPPH scavenging activity of Arum dioscoridis. Natural product research, 29(17), 1678-1683, (2015).
• Referans30 Kuang Y., Wang Q., Chen Z., Megharaj M. & Naidu R. Heterogeneous Fenton-like oxidation of monochlorobenzene using green synthesis of iron nanoparticles. J Colloid Interface Sci., 410, 67–73, (2013).
• Referans31 Kumar D., Arya V., Kaur R., Bhat Z. A., Gupta V. K. & Kumar V. A review of immunomodulators in the Indian traditional health care system. Journal of microbiology, immunology, and infection, 45(3), 165–184, (2012).
• Referans32 Machado S., Grosso J. P., Nouws H., Albergaria J. T. & Delerue-Matos C. Utilization of food industry wastes for the production of zerovalent iron nanoparticles. Sci Total Environ. 496, 233–240, (2014).
• Referans33 Mandal V., Mohan Y. & Hemalatha S. Microwave assisted extraction - an innovative and promising extraction tool for medicinal plant research. Pharmacognosy Reviews, 1(1), (2007).
• Referans34 Microsoft Academic. EC50. https://academic.microsoft.com/topic/96529984/publication/search?q=EC50&qe=And(Composite(F.FId%253D96529984)%252CTy%253D%270%27)&f=&orderBy=0 (date of access: 04.11.2021)
• Referans35 Nahrstedt A. Arum: A plant genus with great medicinal potential. Phytochemistry, 14, 1870, (1975).
• Referans36 Obeidat M., Shatnawi M., Al-alawi M., Al-Zubi E., Al-Dmoor H., Al-Qudah M. & Otri I. Antimicrobial activity of crude extracts of some plant leaves. Research Journal of Microbiology, 7(1), 59-67, (2012).
• Referans37 Pekoglu E., Buyukakilli B., Turkseven C. H., Balli E., Bayrak G., Cimen B. & Balci S. Effects of Trans-Cinnamaldehyde on Reperfused Ischemic Skeletal Muscle and the Relationship to Laminin. Journal of investigative surgery : the official journal of the Academy of Surgical Research, 1–10, (2020).
• Referans38 Sajem A. L. & Gosai K. Traditional use of medicinal plants by the Jaintia tribes in North Cachar Hills district of Assam, northeast India. Journal of ethnobiology and ethnomedicine, 2(1), 1-7, (2006).
• Referans39 Türkmen F. U., Takcı H. A. M., Onalan F. E. S. & Saglam H. Arum dioscoridis ekstraktlarının toplam fenolik, flavonoid içerikleri ile antioksidan ve antibakteriyel aktivitelerinin araştırılması. Harran Üniversitesi Mühendislik Dergisi, 4(1), 102-108, (2019).
• Referans40 Tunçtürk M. & Özgökçe F. Chemical composition of some Apiaceae plants commonly used in herby cheese in Eastern Anatolia. Turkish Journal of Agriculture and Forestry, 39(1), 55-62, (2015).
• Referans41 Turkseven C. H., Buyukakilli B., Balli E., Yetkin D., Erdal M. E., Yilmaz S. G. & Sahin L. Effects of Huperzin-A on the Beta-amyloid accumulation in the brain and skeletal muscle cells of a rat model for Alzheimer's disease. Life sciences, 184, 47–57, (2017).
• Referans42 Tuzlacı E. Şifa Niyetine. İstanbul: Alfa Yayınları, (2006).
• Referans43 Uguzlar H., Maltas E. & Yıldız S. Screening of phytochemicals and antioxıdant activity of Arum dioscoridis seeds. Journal of Food Biochemistry, 1745-4514, (2011).
• Referans44 U.S. National Plant Germplasm System: https://npgsweb.ars-grin.gov/gringlobal/taxon/taxonomysimple.aspx (date of access:: 21.12.2019)]
• Referans45 Wang T., Lin J., Chen Z., Megharaj M. & Naidu R. Green synthesized iron nanoparticles by green tea and eucalyptus leaves extracts used for removal of nitrate in aqueous solution. Journal of cleaner production. 83, 413-419, (2014).
• Referans46 World Health Organization. WHO global report on traditional and complementary medicine 2019. World Health Organization, (2019).
• Referans47 Yabalak E. Radical scavenging activity and chemical composition of methanolic extract from Arum dioscoridis SM. var. dioscoridis and determination of its mineral and trace elements. JOTCSA. 5(1): 205-218, (2017).