ACINETOBACTER CALCOACETICUS- ACINETOBACTER BAUMANNII (ABC) AND NEW SPECIES

Öz

The Acinetobacter genus had 12 genomic species. In the last 30 years based on DNA-DNA hybridization the taxonomic differences has been reported. Currently 49 species are recognized. The biochemically indistinguishable strains, A. baumannii (genomik species 2), Acinetobacter nosocomialis (genomik species 13TU), Acinetobacter pittii (genomik species 3) and Acinetobacter calcoaceticus (genomik species 1) are the clinical important species in the A.calcoaceticus- A.baumannii complex (ABC). Two nowel pathogenic species, Acinetobacter seifertii and Acinetobacter djikshoorniae has been included to A. calcoaceticus- A.baumannii complex by DNA-DNA hybridizaition method, rpoB gene sequence analysis and multilocus sequence typing (MLST) methods. Draft genome sequence of a pathogenic strain JVAP01T, model strain of A. djikshoorniae, which a conjugative plasmid with blaNDM-1 gene has been reported. RNA polimerase β-subunit (rpoB)gene, DNA gyrase B (gyrB) gene sequence analysıs and /or multilocus sequence analysis (MLSA) are known as gold standard molecular methods for the idenitfication at species level. Others are amplified ribosomal DNA restriction analysis (ARDRA), Fourier transform infrared spectroscopy (FTIR) and Matriks assisted lazer desorption ionization time of flight mass spectrometry (MALDI-TOF MS). There is critical importance in keeping the development of antimicrobial resistance of Acinetobacter species at lowest levels such as using relaible laboratory methods, continous infection control measures and rationale antibiotic strategies.

Anahtar Kelimeler

• A. calcoaceticus- A. baumannii complex,Acinetobacter seifertii,Acinetobacter djikshoorniae

ACINETOBACTER CALCOACETICUS- ACINETOBACTER BAUMANNII COMPLEX (ABC) VE YENİ TÜRLERİ

Öz

Acinetobacter cinsi’nin sınıflandırılmasında 12 farklı genomik tür bulunduğu gösterilmiş, taksonomisinde son 30 yılda DNA-temelli metotların yaygın kullanımı sayesinde değişiklikler kaydedilmiştir. Bugün için Acinetobacter cinsi içerisinde 49 tür bulunmaktadır. A. calcoaceticus- A.baumannii complex içinde (ABC) klinik olarak önemli türler olan A. baumannii (genomik tür 2), Acinetobacter nosocomialis (genomik tür 13TU), Acinetobacter pittii (genomik tür 3) ve Acinetobacter calcoaceticus (genomik tür 1) türleri biyokimyasal olarak ayırd edilemezler. DNA-DNA hibridizasyon, rpoB gen sekans analizi ve multilocus sequence typing (MLST) ile A. calcoaceticus- A. baumannii complex’e son yıllarda iki yeni patojenik tür olan Acinetobacter seifertii ve Acinetobacter djikshoorniae eklenmiştir. Ayrıca non-A.baumannii türleri arasında plazmide lokalize blaNDM-1 geni taşıyan ve A. djikshoorniae’nın model suşu olan JVAP01T suşunun taslak genom dizilimi de yayınlanmıştır. Bugün için; Acinetobacter cinsinin tür düzeyinde ayrımı için; RNA polimeraz β-subunit (rpoB) geni, DNA giraz B (gyrB) geni sekans analizi ve/veya multilocus sequence analysis (MLSA) altın standard moleküler metotlar olarak kabul edilmektedir. Diğer yöntemler; amplified ribosomal DNA restriction analysis (ARDRA), Fourier transform infrared spectroscopy (FTIR) , ve Matriks assisted lazer desorption ionization time of flight mass spectrometry (MALDI-TOF MS)’dur. Acinetobacter türlerine karşı antimikrobiyal direnç gelişimini önlemek için izolatların tür düzeyinde güvenilir yöntemler kullanılarak tanımlanmaları, uygun antibiyotiklerin kullanılması ve kesintisiz enfeksiyon kontrol önlemlerinin uygulanması önemlidir.

Anahtar Kelimeler

• A.calcoaceticus- A.baumannii complex,Acinetobacter seifertii,Acinetobacter djikshoorniae

Kaynakça

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