MULTİPLE SKLEROZ HASTALARINDA APOE GEN EKSPRESYONUNUN BELİRLENMESİ

Öz

AMAÇ: Apolipoprotein E (ApoE), lipoprotein parçacıklarının reseptör aracılı endositozunda ligand olarak işlev gören bir glikoproteindir. ApoE'nin Alzheimer ve multipl skleroz gibi nörojeneratif hastalıklar ile ilişkisini gösteren çalışmalar vardır. Bu çalışmanın amacı ApoE gen ekspresyonu ile MS arasında bir ilişki olup olmadığını araştırmaktır.
GEREÇ VE YÖNTEM: ApoE gen ekspresyonunun multiple skleroz hastlığı üzerinde etkisini saptamak amacıyla yaptığımız çalışmamıza 35 hasta ve 20 sağlıklı birey dahil edilmiştir. ApoE mRNA ekspresyonunun seviyesi, Revers-Transkriptaz Polimeraz Zincir Reaksiyonu (qRT-PCR) ile belirlendi. İstatiksel analiz için SPSS paket programı kullanılarak,t testi, ki-kare testi, Kruskal-Wallis testi ve Mann-Whitney U testi yapıldı ve p <0,05 istatistiksel olarak anlamlı kabul edildi.
BULGULAR: Sonuç olarak, ApoE gen ekspresyonunun çoklu skleroz hastalarında ve sağlıklı kişilerin gerçek zamanlı PCR teknikleri kullanılarak karşılaştırılması, gruplar arasında istatistiksel olarak anlamlılık göstermedi (p =0.95).
SONUÇ: Çalışmamız ApoE gen ekspresyonunun MS hastalığı ile ilişkili olabileceğini desteklememektedir. ApoE'nin Multipl Skleroz hastalığında rolünü belirlemek için ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• APOE
• multiple sklerozis
• gen ekspresyonu

DETERMINATION OF APOE GENE EXPRESSION IN MULTIPLE SCLEROSIS PATIENTS

Öz

OBJECTIVE: Apolipoprotein E (ApoE) is a glycoprotein that functions as a ligand in receptor-mediated endocytosis of lipoprotein particles. There are studies showing the association of APOE with neurogenerative diseases such as Alzheimer's disease and multiple sclerosis (MS). The aim of this study was to investigate whether there is a relationship between APOE gene expression and MS.
MATERIAL AND METHODS: To determine the effect of APOE gene expression on multiple sclerosis patients, we have included 35 MS patients and 20 healthy subjects into the study. The level of APOE mRNA expression was determined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). For statistical analysis, t-test, chi-square test, Kruskal-Wallis test and Mann-Whitney U test were performed using SPSS package program and p <0.05 was considered as statistically significant.
RESULTS: As a result, the comparison of ApoE gene expression in multiple sclerosis patients and healthy people using real-time PCR technique did not show any statistical significance between the groups (p=0.95).
CONCLUSIONS: Our study does not support that ApoE gene expression may be related to MS. Further studies are needed to determine the role of ApoE in Multiple Sclerosis disease.

Anahtar Kelimeler

• APOE
• multiple sclerosis
• Gene expression

Teşekkür

We thank to the staff of the Neurology Unit of Çanakkale Onsekiz Mart University Research and Education Hospital for collecting blood samples from patients with MS.

Kaynakça

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