The effects of hydrogen sulfide, carbon monoxide and nitric oxide on pentylenetetrazole-induced seizures in rats

Ezgi Eroğlu; Çiğdem Çengelli Ünel; Nuşin Harmancı; Zuhal Kaltuş; Cansu Kılıç; Hatice Doğan; Rabiye Altınbaş; Kevser Erol

doi:10.17517/ksutfd.681282

EN TR

The effects of hydrogen sulfide, carbon monoxide and nitric oxide on pentylenetetrazole-induced seizures in rats

Abstract

Objective: It is aimed to investigate the anti-convulsant effects of bioactive gas mediators hydrogen sulfide (H2S), carbon monoxide (CO) and nitric oxide (NO) in pentylenetetrazole-induced convulsions in rats. Material Methods: Eighty male Wistar-Albino rats randomly divided into ten groups. Convulsions was induced by administering 60 mg/kg pentylentetrazole intraperitonally. Diazepam, NaHS, CORM-2, L-arginine and L-NAME were administred 60 minutes after pentylentetrazole. Seizure latency, seizure duration and seizure score were evaluated. Data were analyzed by the Kruskal Wallis test followed by posthoc analyses with Tukey. P <0.05 was considered statistically significant. Results: Diazepam (2 mg/kg) extended the seizure latency and no significant difference was found in other groups. Diazepam (1 and 2 mg/kg) and NaHS (10 mg/kg) reduced seizure duration. NaHS (5 mg/kg), CORM-2 (5 and 10 mg/kg), L-arginine (30 and 100 mg/kg) and L-NAME (30 mg/kg) increased the seizure score significantly as compared to diazepam (1 and 2 mg/kg) groups. Conclusion: NaHS (10 mg/kg) may be protective in pentylentetrazole-induced seizures.

Keywords

epilepsy , hydrogen sulfide , carbon monoxide , nitric oxide , seizures

Hidrojen sülfür, karbon monoksit ve nitrik oksidin sıçanlarda pentilentetrazol indüklü nöbetler üzerindeki etkileri

Öz

Amaç: Sıçanlarda pentilentetrazol indüklü konvülsiyonlarda biyoaktif gaz mediyatörler hidrojen sülfür (H2S), karbon monoksid (CO) ve nitrik oksid (NO)'in anti-konvülsan etkilerinin araştırılması amaçlanmıştır. Gereç Yöntem: Seksen erkek Wistar-Albino sıçan rastgele on gruba ayrılmıştır. Konvülsiyonlar, intraperitonal olarak verilen 60 mg/kg pentilentetrazol ile indüklendi. Pentilentetrazolden 60 dakika sonra Diazepam, NaHS, CORM-2, L-arginin ve L-NAME uygulandı. Nöbet latensi, nöbet süresi ve nöbet skoru değerlendirildi. Veriler Kruskal Wallis testi ve ardından Tukey ile analiz edildi. P <0.05 istatistiksel olarak anlamlı kabul edildi. Bulgular: Diazepam (2 mg/kg) nöbet latensini uzattı, diğer gruplarda ise anlamlı bir fark bulunmadı. Diazepam (1 ve 2 mg/kg) ve NaHS (10 mg/kg) nöbet süresini azalttı. NaHS (5 mg/kg), CORM-2 (5 ve 10 mg/kg), L-Arjinin (30 ve 100 mg / kg) ve L-NAME (30 mg / kg), nöbet skorunu diazepam ile karşılaştırıldığında önemli ölçüde artırdı. Sonuç: NaHS (10 mg/kg) pentilentetrazole bağlı nöbetlerde koruyucu olabilir.

Anahtar Kelimeler

epilepsi , hidrojen sülfür , karbon monoksid , nitrik oksid , nöbetler

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Ezgi Eroğlu ^*
0000-0002-9878-3431
Türkiye

Çiğdem Çengelli Ünel Bu kişi benim
0000-0001-7680-0141
Türkiye

Nuşin Harmancı
0000-0002-5777-2162
Türkiye

Zuhal Kaltuş Bu kişi benim
0000-0003-2281-1712
Türkiye

Cansu Kılıç Bu kişi benim
0000-0003-1100-9417
Türkiye

Hatice Doğan
0000-0002-9616-8910
Türkiye

Rabiye Altınbaş
0000-0003-2535-0480
Türkiye

Kevser Erol
0000-0002-8808-6616
Türkiye

Yayımlanma Tarihi

16 Ekim 2020

Gönderilme Tarihi

29 Ocak 2020

Kabul Tarihi

13 Mart 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 15 Sayı: 3

DOI

https://doi.org/10.17517/ksutfd.681282

IZ

https://izlik.org/JA57PL39TR

AMA

1.Eroğlu E, Çengelli Ünel Ç, Harmancı N, vd. The effects of hydrogen sulfide, carbon monoxide and nitric oxide on pentylenetetrazole-induced seizures in rats. KSÜ Tıp Fak Der. 2020;15(3):1-6. doi:10.17517/ksutfd.681282

The effects of hydrogen sulfide, carbon monoxide and nitric oxide on pentylenetetrazole-induced seizures in rats

Abstract

Keywords

Hidrojen sülfür, karbon monoksit ve nitrik oksidin sıçanlarda pentilentetrazol indüklü nöbetler üzerindeki etkileri

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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