Efficacy of P-Coumaric acid and Ellagic acid added to preservation solutions in transplantation

Yıl 2021, , 198 - 207, 07.07.2021

Fatih Mehmet Yazar , Aykut Urfalıoğlu , Ömer Faruk Boran , Abdulkadir Bahar Hasan Dağlı , Mehmet Gül Fatma İnanç Tolun , Ertan Bulbuloglu

https://doi.org/10.17517/ksutfd.781757

Öz

OBJECTIVE: In this study, it was aimed to evaluate the protective efficacy of the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) preservation solutions enriched with P-coumaric acid and ellagic acid.
MATERIAL AND METHOD
Thirty-six Wistar albino male rats were divided into equal 6ng groups: (Group 1) UW; (Group 2) UW + P-coumaric; (Group 3) UW + Elagic; (Group 4) HTK ;. (Group 5) HTK + P-Coumaric; (Group 6) HTK + Elagic. After the liver perfusion was performed, samples were taken from the liver tissue and perfusion fluid at 0, 6 and 12 hours. At the sixth hour, liver biopsies were performed for histopathological and electron microscopic examination.
RESULTS
In groups enriched with p-coumaric acid (group 2 and group 5), antioxidant enzyme activities, which were higher than the control groups, were decreased at the 0th hour and the difference disappeared at the 6th and 12th hour. In the groups enriched with Ellagic acid, the highest antioxidant enzyme activity was in group 6, when the groups were evaluated in terms of cytokine activity, which are inflammatory parameters, the group with the highest anti-inflammatory cytokines IL4 and IL10 was found to be in group 6, and in group 3, the results were found with group 6. it was found to be similar. In group 2 and group 5, although the results were lower than the control group, the difference was not significant (P> 0.05). It was observed that the results in group 6, in which the lowest values ​​in terms of inflammatory cytokines TNF-α, IL1 and IL6 were in group 3, were higher than in group 3, but significantly lower than the control group, especially in terms of IL6 values ​​(P <0.05). In the electron microscopic examination, it was seen that the groups to which ellagic acid was added had the lowest damage level at the cellular level compared to the control groups, although the cellular damage results in group 2 and group 5 were lower than the control groups, they were not statistically different.
RESULT
The addition of Elagic acid and P-Coumaric acid to preservation solutions caused protective effects on the transplanted liver tissue.

Anahtar Kelimeler

liver transplantation, preservation solution, antioxidant, histidine-tryptophan-ketoglutarate, P-coumaric acid, ellagic acid, solution of university of Wisconsin

Destekleyen Kurum

This project was supported by the KSU Research Projects Management Unit.

Proje Numarası

(Project number: 2015/1-59M)

Transplantasyonda koruma solüsyonlarına eklenen P-Coumaric asit ve Ellagic asit etkinliği

Öz

AMAÇ: Bu çalışmada, P-kumarik asit ve ellagik asit ile zenginleştirilmiş Wisconsin Üniversitesi (UW) ve histidin-triptofan-ketoglutarat (HTK) koruma solüsyonlarının koruyucu etkinliğininin değerlendirilmesi amaçlanmıştır.
MATERYAL ve METOD: Otuz altı Wistar albino erkek sıçan eşit 6ngruba ayrıldı: (Grup 1) UW; (Grup 2) UW + P-coumaric; (Grup 3) UW+Elagic; (Grup 4) HTK;. (Grup 5) HTK + P-Coumaric; (Grup 6) HTK + Elagic. Karaciğer perfüzyonu yapıldıktan sonra 0, 6 ve 12. saatte karaciğer dokusundan ve perfüzyon sıvısından örnekler alındı. Altıncı saatte histopatolojik ve elektron mikroskopik inceleme için KC biyopsileri yapıldı.
BULGULAR: P-coumaric asit ile zenginleştirilmiş gruplarda (grup 2 ve grup5) 0. saatte kontrol gruplarına göre yüksek olan antioksidan enzim aktivitelerinin azaldığı ve 6 ve 12. saatte farkın ortadan kalktığı görüldü. Ellagic asit ile zenginleştirilen gruplarda ise en yüksek en yüksek antioksidan enzim aktivitesinin grup 6’da olduğu, gruplar inflamatuar parametreler olan sitokin aktivitesi açısından değerlendirildiğinde antiinflamatuar sitokinler olan IL4 ve IL10 değerinin en yüksek olduğu grubun grup 6 olduğu, grup 3 de de sonuçların grup 6 ile benzer olduğu görüldü. Grup 2 ve grup 5’de ise sonuçların kontrol grubuna göre düşük olmasına rağmen farkın anlamlı olmadığı tespit edildi (P>0.05). İnflamatuar sitokinler olan TNF- α, IL1 ve IL6 açısından en düşük değerlerin grup 3’de olduğu grup 6 daki sonuçların ise grup 3’e göre yüksek olmakla birlikte özellikle IL6 değerleri açısından kontrol grubuna göre anlamlı oranda düşük olduğu görüldü (P<0.05). Elektron mikroskobik incelemede, ellagic asit eklenen grupların kontrol gruplarına göre hücresel düzeyde en düşük hasar düzeyine sahip olduğu, grup 2 ve grup 5’deki hücresel hasar sonuçlarının kontrol gruplarına göre düşük olmasına rağmen istatistiksel olarak farklı olmadığı görüldü
SONUÇ: Elagic asit ve P-Coumaric asitin prezervasyon solusyonlarına eklenmesi transplante edilecek KC dokusu üzerine koruyucu etkilere neden olmuştur.

Anahtar Kelimeler

karaciğer nakli, koruma solüsyonları, histidin-triptofan-ketoglutarat, Wisconsin Üniversitesi solüsyonu, antioksidan, ellagik asit, p-kumarik asit

Proje Numarası

(Project number: 2015/1-59M)

Kaynakça

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