Astımlı Çocuklarda Atak Sırasında Plazma Paraoksonaz Düzeylerinin ve PON-1 gen Polimorfizminin Önemi

Yıl 2021, , 322 - 326, 01.11.2021

İrfan Şahin , Osman Gülsever

https://doi.org/10.17517/ksutfd.813729

Öz

Amaç: Paraoksonaz 1 (PON-1) enzimi önemli bir antioksidandır. Astım atakları sırasında oksidatif stres ve antioksidan savunma mekanizmaları arasındaki denge bozulur. PON-1 enziminin aktivitesi, PON-1 gen polimorfizmi ile ilişkilidir. Bu çalışma astımlı çocuklarda PON-1 enzimi ve Q192R polimorfizminin aktivitesini değerlendirmeyi amaçlamaktadır.
Gereç ve yöntemler: Çalışma 2013-2014 yılları arasında 34 astımlı (çalışma grubu) ve 34 sağlıklı (kontrol grubu) çocuğun prospektif olarak değerlendirilmesiyle yapıldı. Serum PON-1 aktivitesi, çalışma grubunda astım atağının başlangıcında ve sonunda ve ayrıca kontrol grubunda değerlendirildi. Astımlı çocuklarda PON-1 enzim aktivitesini etkilediği bilinen Q192R gen polimorfizmi de araştırıldı.
Bulgular: Astımlı çocuklar ve sağlıklı kontroller arasında serum PON-1 enzim aktivitesi ve PON-1 gen polimorfizmi açısından anlamlı fark yoktu. Bununla birlikte, astımlı çocukların PON-1 enzim aktivitesinin astım atağı sonunda astım atağı başlangıcına göre önemli derecede arttığı gözlendi.
Sonuç: Astımlı çocuklarda PON-1 enzim aktivitesi ve Q192R genetik polimorfizmi sağlıklı çocuklardan farklı değildir, ancak PON-1 enzim aktivitesi astım atağının sonunda atağın başlangıcına göre daha yüksektir. Antioksidan mekanizmanın bir üyesi olan PON-1 enzimi, hem astım patofizyolojisini anlamada hem de yeni tedavilerin geliştirilmesinde önemli olabilir.

Anahtar Kelimeler

Paraoksonaz, Genetik Polimorfizm, Astım, Çocuklar

Paraoxonase 1: Could it be important for asthma in children?

Öz

Objective: The paraoxonase 1 (PON-1) enzyme is an important antioxidant. The balance between oxidative stress and antioxidant defense mechanisms is disturbed during asthma attacks. The activity of the PON-1 enzyme is related to PON-1 gene polymorphism. This study aimed to evaluate the activity of PON-1 enzyme and Q192R polymorphism in asthmatic children.
Material and methods: During the 2013-2014 period, we performed a prospective study with 34 asthmatic (study group) and 34 healthy (control group) children. Serum PON-1 activity was evaluated in the study group at the onset of an asthma attack and at the end, and also in the control group. Q192R gene polymorphism of asthmatic children, which is known to affect the PON-1 enzyme activity was also investigated.
Results: There was no significant difference in terms of serum PON-1 enzyme activity and PON-1 gene polymorphism between the asthmatic children and healthy controls. However, a significant increase was observed at the PON-1 enzyme activity of asthmatic children at the end of the asthma attack compared to its onset.
Conclusion: The PON-1 enzyme activity and Q192R genetic polymorphism in asthmatic children are not different from those of healthy children, but PON-1 enzyme activity is higher at the end of an asthma attack compared to the onset. PON-1 enzyme, as a member of the antioxidant mechanism, may be important both in understanding the pathophysiology of asthma and in the development of new medications for asthma.

Anahtar Kelimeler

Paraoxonase, Genetic Polymorphism, Asthma, Children

Kaynakça

• 1. Soriano JB, Abajobir AA, Abate KH, Abera SF, Agrawal A, Ahmed MB, et al. Global, regional, and national deaths, prevalence, disability adjusted life years, and years lived with disability for chronic obstructive pulmonary disease and asthma, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet Respir Med 2017;5:691–706.
• 2. Jesenak M, Zelieskova M, Babusikova E. Oxidative Stress and Bronchial Asthma in Children—Causes or Consequences? Front Pediatr 2017;5:162.
• 3. El-Alameey IR, Fathy GA, Shady MMA, Ali A, Fathy HA, Youness ER, et al. Relationship of Oxidant and Antioxidant Markers to Asthma Severity in Egyptian Asthmatic Children. Maced J Med Sci 2017;15(5):645-650.
• 4. Litvinov D, Mahini H, Garelnabi M. Antioxidant and anti-inflammatory role of paraoxonase 1: implication in arteriosclerosis diseases. N Am J Med Sci. 2012;4(11):523-32.
• 5. Chiesa ST, Charakida M. High-Density Lipoprotein Function and Dysfunction in Health and Disease. Cardiovasc Drugs Ther 2019;33(2):207-219.
• 6. Lüersen K, Schmelzer C, Boesch-Saadatmandi C, Kohl C, Rimbach G, Döring F. Paraoxonase 1 polymorphism Q192R affects the pro-inflammatory cytokine TNF-alpha in healthy males. BMC Res Notes 2011;4:141.
• 7. Bijanzadeh M, Mahesh PA, Ramachandra NB. An understanding of the genetic basis of asthma. Indian J Med Res 2011;134:149-161.
• 8. Phaniendra A, Jestadi DB, Periyasamy L. Free radicals: properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem 2015;30(1):11-26.
• 9. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5(1):9-19.
• 10. Shunmoogam N, Naidoo P, Chilton R. Paraoxonase (PON)-1: a brief overview on genetics, structure, polymorphisms and clinical relevance. Vasc Health Risk Manag 2018;14:137-143.
• 11. Abuhandan M, Cakmak A, Taskın A, Karakaya E, Kocyigit A, Kılıc H. The effect of age on the paraoxonase and arylesterase activity of pregnant mothers and their infants. J Clin Lab Anal 2012;26(4):302-6.
• 12. Levy D, Reichert CO, Bydlowski SP. Paraoxonases Activities and Polymorphisms in Elderly and Old-Age Diseases: An Overview. Antioxidants 2019;8(5):118.
• 13. Karakaya P, Ozdemir B, Mert M, Okuturlar Y. Relation of Paraoxonase 1 Activity with Biochemical Variables, Brachial Artery Intima-Media Thickness in Patients with Diabetes with or without Obesity. Obes Facts 2018;11(1):56-66.
• 14. Cakmak A, Zeyrek D, Atas A. Oxidative status and paraoxonase activity in children with asthma. Clin Invest Med 2009;32:327-334.
• 15. Sarioglu N, Hismiogullari AA, Erel F, Demir D, Gencer N. Paraoxonase 1 phenotype and paraoxonase activity in asthmatic patients. Iran J Allergy Asthma Immunol 2015;14:60-66.
• 16. Gürbüz Ş, Yıldız M, Kara M, et al. Paraoxonase-1 gene in patients with chronic obstructive pulmonary disease investigation Q192R and L55M polymorphisms. World J Emerg Med 2015;6(3):201-206.
• 17. Luo JQ, Ren H, Liu MZ, Fang PF, Xiang DX. European versus Asian differences for the associations between paraoxonase-1 genetic polymorphisms and susceptibility to type 2 diabetes mellitus. J Cell Mol Med 2018;22(3):1720-1732.