Ratlarda Deneysel Olarak Oluşturulan Kolit Modelinde N-Asetil Sistein ve β-Glukanın Etkileri

Murat İspiroğlu; Bülent Kantarçeken; Harun Çıralık; Ertan Bulbuloglu

doi:10.17517/ksutfd.817235

EN TR

Effects of N-Acetyl Cystein And Beta Glucan in Experimental Colitis Model in Rats

Abstract

Introduction and Aim Ulcerative colitis is an idiopathic and chronic disease characterized by diffuse mucosal inflammation. Oxidant and antioxidant balance is impaired in tissue. The aim of the present study was to evaluate effects of N-acetylcysteine and β-glucan against colitis. Materials and Methods 220-250 g average, 50 male Wistar-Albino rats, were used. Single dose rectal acetic acid was applied for colitis. β-glucan 100 mg/kg/day and N-acetylycteine 200mg/kg/day were given with oral probe. Group I (Control): Single dose rectal saline+normal diet. Group II (Colitis): Single dose rectal acetic acid+normal diet Group III (β-Glucan+Colitis): Single dose β-glucan 100 mg/kg (1 hour before rectal asetic acid) + Rectal acetic acid + β-glucan 100 mg / kg / day (during next 6 days). Group IV(N-asetilsistein+Colitis): Single dose N-acetylycteine 200 mg/kg (one hour before rectal asetic acid) + Rectal acetic acid + N-acetylycteine 200 mg/kg/day (during next 6 days). Group V (N-asetilsistein+β-glucan+Colitis): Single dose N-acetylycteine 200 mg/kg and beta-glucan 100 mg/kg/day (one hour before rectal asetic acid) + Rectal acetic acid + N-acetylycteine 200 mg/kg/day and beta-glucan 100 mg/kg/day (during next 6 days). End of the study, distal 8 cm colon was removed. Histopathologic and biochemical assesments were carried out. Results MDA and MPO levels were significantly low, SOD and KAT levels were significantly higher in monotherapy and combined therapy groups. MPO and MDA levels were not statistically different between treatment groups. For other anti-oxidant enzymes (SOD, KAT), there were no difference between NAC and BG group, but in combination group anti-oxidant defence was lower than NAC group. Microscobic evaluation revealed that damage score was lowest in NAC group, but no statistical significance was found between treatment groups. Conclusions This study suggest that N-asetilsistein and/or b-glukan treatment modalities have beneficial effect in colitis. But combined therapy compared with monotherapy did not show additional beneficial effect.

Keywords

Beta-glucan , N-Acetylcysteine , oxidative stress , experimental colitis

Ratlarda Deneysel Olarak Oluşturulan Kolit Modelinde N-Asetil Sistein ve β-Glukanın Etkileri

Öz

Giriş-Amaç Ülseratif kolit, kolonu diffüz tutan mukozal inflamasyonla karakterize rekürren, idiyopatik ve kronik bir hastalıktır. Kolitde, oksidan/antioksidan dengenin bozulduğu gözlenmiştir. N-asetilsistein ve β-glukan ise antioksidan, anti-inflamatuvar özellikte olan maddeler olup kolitde yararlı etkilerini araştırmayı amaçladık. Materyal-Metod 220-250 gr 50 adet erkek Wistar Albino rat kullanıldı. Grup I (Kontrol):Tek doz rektal saline uygulandı, sonrası 6 gün normal besin verildi. Grup II (Kolit):Tek doz rektal asetik asit uygulandı, sonrası 6 gün normal besin verildi. Grup III (β-Glukan+Kolit):Tek doz oral 100mg/kg β-glukan verildikten 1 saat sonra rektal asetik asit uygulandı. Sonraki 6 gün oral 100/mg/kg/gün β-glukan verildi. Grup IV (N-asetilsistein+Kolit):Tek doz oral 200mg/kg N-asetilsistein verildikten 1 saat sonra rektal asetik asit uygulandı. Sonraki 6 gün oral 200mg/kg/gün N-asetilsistein verildi. Grup V (N-asetilsistein+β-glukan+Kolit): Tek doz oral 200mg/kg N-asetilsistein+100 mg/kg β-glukan verildi. 1 saat sonra rektal asetik asit uygulandı. Sonraki 6 gün oral 100 mg/kg/gün β-glukan + 200 mg/kg/gün N-asetilsistein verildi. Çalışmanın sonunda kalın barsak distal 8 cm’lik kısmı çıkarıldı. Histopatolojik ve biyokimyasal inceleme için örnekler alındı. Bulgular Tedavi alanlar da almayanlara göre MDA ve MPO düzeyleri anlamlı düşük; SOD, KAT düzeyleri ise anlamlı yüksek olduğu gözlendi. Tedavi grupları arasında, MDA ve MPO düzeylerinde anlamlı fark yoktu. Diğer antioksidan enzimler (SOD, KAT); N-asetilsistein grubuyla β-glukan grubu arasında anlamlı fark yok iken kombinasyon verilen grupta antioksidan savunma, N-asetilsistein grubundan anlamlı olarak düşük gözlendi. Histopatolojik skor ortalamaları değerlendirildiğinde kontrol ve tedavi grupları arasında istatiksel olarak anlamlı fark izlenmedi. Sonuçlar N-Asetilsistein ve β-glukan’ın kolitde faydalı olduğu izlendi. N-asetilsistein ve β-glukan ‘ın kombine verilmesinin ek bir fayda sağlamadığı gözlendi.

Anahtar Kelimeler

Beta-glukan , N-Asetilsistein , oksidatif stres , deneysel kolit

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Murat İspiroğlu ^*
0000-0002-0655-7235
Türkiye

Bülent Kantarçeken
0000-0003-4214-817X
Türkiye

Harun Çıralık
0000-0001-5100-3277
Türkiye

Ertan Bulbuloglu
0000-0001-7798-5010
Türkiye

Yayımlanma Tarihi

1 Kasım 2021

Gönderilme Tarihi

27 Ekim 2020

Kabul Tarihi

10 Kasım 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 16 Sayı: 3

DOI

https://doi.org/10.17517/ksutfd.817235

IZ

https://izlik.org/JA46TZ36LH

AMA

1.İspiroğlu M, Kantarçeken B, Çıralık H, Bulbuloglu E. Ratlarda Deneysel Olarak Oluşturulan Kolit Modelinde N-Asetil Sistein ve β-Glukanın Etkileri. KSÜ Tıp Fak Der. 2021;16(3):357-364. doi:10.17517/ksutfd.817235

Cited By

Deneysel Sıçan Kolit Modelinde Alfa Lipoik Asit Tedavisinin SOD ve NLRP3 Seviyesi Üzerine Etkisi

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi

https://doi.org/10.62425/jlasp.1565052

Effects of N-Acetyl Cystein And Beta Glucan in Experimental Colitis Model in Rats

Abstract

Keywords

Ratlarda Deneysel Olarak Oluşturulan Kolit Modelinde N-Asetil Sistein ve β-Glukanın Etkileri

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Deneysel Sıçan Kolit Modelinde Alfa Lipoik Asit Tedavisinin SOD ve NLRP3 Seviyesi Üzerine Etkisi