Araştırma Makalesi
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Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts

Yıl 2021, Cilt: 16 Sayı: 2, 231 - 235, 07.07.2021
https://doi.org/10.17517/ksutfd.788445

Öz

Objective: In Psephellus and Centaurea genuses a lot of species have important pharmacological activities like antipyretic, antiinflamatory, diuretic, cytotoxic etc. In Psephellus genus the endemic plant Psephellus pyrrhoblepharus (Boiss.) Wagenitz (Centaurea pyrrhoblephara) was collected from Elazığ, Turkey. Recently interest in use of plant-derived compounds for therapeutic purposes in cancer is increasing day by day. Liver cancer which is one of the cancer types is affecting millions of people all over the world. So any approach to treat liver cancer is extremely valuable. Materials and methods: We have purposed to determine the cytotoxic activity of methanol:water (1:1), n-hexane and chloroform extracts of the aerial parts of P. pyrrhoblepharus using human liver cancer cell (HepG2) by utilizing the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay in different concentrations (50, 100 ,200 µg/ml) and time points (6, 12, 24 h). Results: Regarding the cytotoxicity, cell viability was seen as decreased following extract exposures at three different time points. The highest cytotoxic activity was observed in 100 µg/ml concentrations of chloroform extract at 24 h. At 12 h chloroform extract of plant showed the highest cytotoxic activity in 200 µg/ml concentrations when compared to methanol:water and n-hexane extracts. Conclusion: It can be suggested that extracts of P. pyrrhoblepharus show dose-dependent and time-dependent cytotoxic effects in HepG2 carsinoma cells. In general, the results provide information about the threshold concentrations of P. pyrrhoblepharus extracts that might be used in different applications without the risk of toxicity.

Kaynakça

  • 1. Akkol EK, Arif R, Ergun F, Yesilada E. Sesquiterpene lactones with antinociceptive and antipyretic activity from two Centaurea species. J Ethnopharmacol 2009; 122(2): 210–215.
  • 2. Vajs V, Todorović N, Ristić M, Tešević V, Todorović B, Janaćković P, et al. Guaianolides from Centaurea nicolai: antifungal activity. Phytochemistry 1999; 52(3): 383–386.
  • 3. Bakr RO, Mohamed SA, Ayoub N. PHENOLIC PROFILE OF Centaurea aegyptiaca L. growing in Egypt and its cytotoxic and antiviral activities. African J Tradit Complement Altern Med 2016; 13(6): 135–143.
  • 4. Radan M, Carev I, Tesevic V, Politeo O, Culic VC. Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extracts. Chem Biodivers 2017; 14(9).
  • 5. Djeddi S, Chatter R, Karioti A, Argyropoulou C, Kharrat R, Skaltsa H. Analgesic activity of secondary metabolites from two Centaurea species. Planta Med 2008; 74(9): 37.
  • 6. Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food Chem Toxicol 2013; 55: 290–296.
  • 7. Pollock JL, Kogan LA, Thorpe AS, Holben WE. (±)-Catechin, A Root Exudate of the Invasive Centaurea Stoebe Lam. (Spotted Knapweed) Exhibits Bacteriostatic Activity Against Multiple Soil Bacterial Populations. J Chem Ecol 2011; 37(9): 1044–1053.
  • 8. Gülcemal D, Alankuş-Çalışkan Ö, Karaalp C, Örs AU, Ballar P, Bedir E. Phenolic Glycosides with antiproteasomal activity from Centaurea urvillei DC. subsp. urvillei. Carbohydr Res 2010; 345(17): 2529–2533.
  • 9. Csupor D, Widowitz U, Blazsõ G, Laczkõ-Zöld E, Tatsimo JSN, Balogh Á, et al. Anti-inflammatory activities of eleven centaurea species occurring in the carpathian basin. Phyther Res 2013; 27(4).
  • 10. Gürbüz I, Yesilada E. Evaluation of the anti-ulcerogenic effect of sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis by using various in vivo and biochemical techniques. J Ethnopharmacol 2007; 112: 284–291.
  • 11. Wagenitz G, Ertugrul K. A new species of Centaurea sect . Psephelloideae ( Compositae ) from SW Turkey. Willdonewia 1998; 28(1-2): 157–161.
  • 12. Wagenitz G, Hellwig FH. The genus Psephellus Cass. (Compositae, Cardueae) revisited with a broadened concept. Willdenowia 2000; 30(1), 29–44.
  • 13. Güner A, Aslan S, Ekim T, Vural M, Babaç M. Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul; 2012.
  • 14. GBD 2015 Disease and Injury Incidence and Prevalence, Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 310 diseases and injuries, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet 2016; 388(10053): 1545–1602.
  • 15. Ashraf A, Sarfraz RA, Rashid MA, Shahid M. Antioxidant, antimicrobial, antitumor, and cytotoxic activities of an important medicinal plant (Euphorbia royleana) from Pakistan. J Food Drug Anal 2015; 13, 109-115.
  • 16. Rashid MA, Akhtar MN, Ashraf A, Nazir S, Ijaz A, Omar NA, Noor S, Basit A, Tareen RB. Chemical composition and antioxidant, antimicrobial and haemolytic activities of Crambe cordifolia roots. Framacia 2018; 66(1), 165-171.
  • 17. Karikas GA. Anticancer and chemopreventing natural products: some biochemical and therapeutic aspects. Journal of BUON : official journal of the Balkan Union of Oncology 2010; 15(4): 627–638.
  • 18. Sonis ST. Oral mucositis. Anticancer Drugs 2011; 22: 607–612.

Psephellus pyrrhoblepharus Ekstrelerinin Sitotoksik Aktivitesi

Yıl 2021, Cilt: 16 Sayı: 2, 231 - 235, 07.07.2021
https://doi.org/10.17517/ksutfd.788445

Öz

Amaç: Birçok Psephellus ve Centaurea türü antipiretik, antiinflamatuar, diüretik ve sitotoksik gibi önemli farmakolojik aktivitelere sahiptir. Endemik bir bitki olan Psephellus pyrrhoblepharus (Boiss.) Wagenitz, Elazığ, Türkiye’den toplanmıştır. Son günlerde kanserde terapötik amaçlar için bitki kaynaklı bileşiklerin kullanımına olan ilgi artmaktadır. Bir kanser türü olan karaciğer kanseri de tüm dünyada milyonlarca insanı etkilemekteyken, karaciğer kanserini tedavi etmek için herhangi bir yaklaşım son derece değerlidir. Bu çalışmada, insan karaciğer kanseri hücresi (HepG2) kullanılarak P. pyrrhoblepharus bitkisinin topraküstü kısımlarından hazırlanmış olan metanol:su (1:1), kloroform ve n-hekzan ekstrelerinin sitotoksik potansiyelini değerlendirilmesi amaçlanmıştır. Gereç ve yöntemler: 3-(4,5-dimetiltiyazol-2-il)-2,5-difenltetrazolyumbromit (MTT) metodu kullanılarak, insan karaciğer kanser hücre hatlarında (HepG2) farklı konsantrasyonlarda (50, 100, 200 ug/ml) MTT, farklı zaman noktalarında (6, 12, 24 s) çalışmalar gerçekleştirildi. Bulgular: Sitotoksisite ile ilgili olarak, farklı zaman noktalarında ekstrelerin maruziyetlerinin ardından hücre canlılığının azaldığı gözlenmiştir. Bununla birlikte, en yüksek sitotoksik aktivite, 24 saatte 100 ug/ml konsantrasyondaki kloroform ekstresinde gözlenmiştir. Bitkinin kloroform ekstresi, metanol:su ve n-hekzan ekstreleri ile karşılaştırıldığında 12 saatte 200 ug/ml konsantrasyonda en yüksek sitotoksik etkinliği göstermiştir. Sonuç: P. pyrrhoblepharus ekstrelerinin HepG2 karsinoma hücrelerinde doza ve zamana bağlı bir şekilde sitotoksik etki gösterdiği ileri sürülebilir. Genel olarak elde edilen sonuçlar, toksisite tehlikesi olmadan farklı uygulamalarda kullanılabilecek P. pyrhoblepharus ekstrelerinin eşik konsantrasyonları hakkında bilgi sağlamıştır.

Kaynakça

  • 1. Akkol EK, Arif R, Ergun F, Yesilada E. Sesquiterpene lactones with antinociceptive and antipyretic activity from two Centaurea species. J Ethnopharmacol 2009; 122(2): 210–215.
  • 2. Vajs V, Todorović N, Ristić M, Tešević V, Todorović B, Janaćković P, et al. Guaianolides from Centaurea nicolai: antifungal activity. Phytochemistry 1999; 52(3): 383–386.
  • 3. Bakr RO, Mohamed SA, Ayoub N. PHENOLIC PROFILE OF Centaurea aegyptiaca L. growing in Egypt and its cytotoxic and antiviral activities. African J Tradit Complement Altern Med 2016; 13(6): 135–143.
  • 4. Radan M, Carev I, Tesevic V, Politeo O, Culic VC. Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extracts. Chem Biodivers 2017; 14(9).
  • 5. Djeddi S, Chatter R, Karioti A, Argyropoulou C, Kharrat R, Skaltsa H. Analgesic activity of secondary metabolites from two Centaurea species. Planta Med 2008; 74(9): 37.
  • 6. Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food Chem Toxicol 2013; 55: 290–296.
  • 7. Pollock JL, Kogan LA, Thorpe AS, Holben WE. (±)-Catechin, A Root Exudate of the Invasive Centaurea Stoebe Lam. (Spotted Knapweed) Exhibits Bacteriostatic Activity Against Multiple Soil Bacterial Populations. J Chem Ecol 2011; 37(9): 1044–1053.
  • 8. Gülcemal D, Alankuş-Çalışkan Ö, Karaalp C, Örs AU, Ballar P, Bedir E. Phenolic Glycosides with antiproteasomal activity from Centaurea urvillei DC. subsp. urvillei. Carbohydr Res 2010; 345(17): 2529–2533.
  • 9. Csupor D, Widowitz U, Blazsõ G, Laczkõ-Zöld E, Tatsimo JSN, Balogh Á, et al. Anti-inflammatory activities of eleven centaurea species occurring in the carpathian basin. Phyther Res 2013; 27(4).
  • 10. Gürbüz I, Yesilada E. Evaluation of the anti-ulcerogenic effect of sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis by using various in vivo and biochemical techniques. J Ethnopharmacol 2007; 112: 284–291.
  • 11. Wagenitz G, Ertugrul K. A new species of Centaurea sect . Psephelloideae ( Compositae ) from SW Turkey. Willdonewia 1998; 28(1-2): 157–161.
  • 12. Wagenitz G, Hellwig FH. The genus Psephellus Cass. (Compositae, Cardueae) revisited with a broadened concept. Willdenowia 2000; 30(1), 29–44.
  • 13. Güner A, Aslan S, Ekim T, Vural M, Babaç M. Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul; 2012.
  • 14. GBD 2015 Disease and Injury Incidence and Prevalence, Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 310 diseases and injuries, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet 2016; 388(10053): 1545–1602.
  • 15. Ashraf A, Sarfraz RA, Rashid MA, Shahid M. Antioxidant, antimicrobial, antitumor, and cytotoxic activities of an important medicinal plant (Euphorbia royleana) from Pakistan. J Food Drug Anal 2015; 13, 109-115.
  • 16. Rashid MA, Akhtar MN, Ashraf A, Nazir S, Ijaz A, Omar NA, Noor S, Basit A, Tareen RB. Chemical composition and antioxidant, antimicrobial and haemolytic activities of Crambe cordifolia roots. Framacia 2018; 66(1), 165-171.
  • 17. Karikas GA. Anticancer and chemopreventing natural products: some biochemical and therapeutic aspects. Journal of BUON : official journal of the Balkan Union of Oncology 2010; 15(4): 627–638.
  • 18. Sonis ST. Oral mucositis. Anticancer Drugs 2011; 22: 607–612.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Pelin Taştan 0000-0003-0913-5369

Güliz Armağan 0000-0001-6466-2263

Taner Dağcı Bu kişi benim 0000-0002-2786-6141

Bijen Kıvçak 0000-0001-7645-1930

Yayımlanma Tarihi 7 Temmuz 2021
Gönderilme Tarihi 31 Ağustos 2020
Kabul Tarihi 23 Aralık 2020
Yayımlandığı Sayı Yıl 2021 Cilt: 16 Sayı: 2

Kaynak Göster

AMA Taştan P, Armağan G, Dağcı T, Kıvçak B. Psephellus pyrrhoblepharus Ekstrelerinin Sitotoksik Aktivitesi. KSÜ Tıp Fak Der. Temmuz 2021;16(2):231-235. doi:10.17517/ksutfd.788445