THE INVESTIGATION OF THE EFFECTS OF VITAMIN D AND OMEGA 3 ON VENA CAVA

Öz

Objective: Vitamin D has a protective role in the cardiovascular system and it affects blood pressure. Omega-3 fatty acids are dietary fats gained from fish and plant oils and involve in coronary heart disease and other cardiovascular complications. The aim of the study was to investigate the effects of Vitamin D and Omega 3 on the vascular structure at the cellular level. Material and Methods: In the current study, a total of 24 rats were divided into 4 groups. Each group contained 6 animals. The groups are as follows; control, vitamin D, Omega 3; and combined Vitamin D and Omega 3. Vena cava samples from all groups were obtained and stained with hematoxylin and eosin (H&E) for histological alternations. Additionally, endothelial and vascular functions were investigated immunohistochemically. Results: The H&E staining revealed that the treatment of either Vitamin D or Omega 3 did not cause histomorphological changes in the structure of the vena cava under normal conditions. The immunoexpression of inducible nitric oxide synthase was decreased and vascular endothelial growth factor was increased in the vena cava of rats with the combined treatment of Vitamin D and Omega 3. Conclusion: In conclusion, combined supplements of Vitamin D and Omega 3 did not have harmful effects on the blood vessel however further studies should be performed to determine the beneficial effects of these supplements.

Anahtar Kelimeler

• Vitamin D
• Omega 3
• histopathology
• vena cava
• inducible nitric oxide synthase
• vascular endothelial growth factor

Vitamin D ve Omega 3’ün Vena Kava Üzerine Etkilerinin Araştırılması

Öz

Amaç: D vitamini kardiyovasküler sistem üzerinde koruyucu bir role sahiptir ve kan basıncını etkiler. Omega-3 yağ asitleri balık ve bitki yağlarından elde edilir ve koroner kalp hastalığına ve diğer kardiyovasküler komplikasyonlarda önemli rolü vardır. Çalışmamızın amacı, D vitamini ve Omega 3'ün damar yapısı üzerindeki etkilerinin hücresel düzeyde araştırmaktır. Gereç ve Yöntemler: Mevcut çalışmada toplam 24 sıçan kullanıldı. Her biri 6 hayvandan oluşan kontrol, D vitamini, Omega 3; ve kombine D Vitamini ve Omega 3 tedavisi uygulanan olmak üzere 4 grup belirlendi. Deney sonunda tüm gruplardan vena cava örnekleri alındı. Dokular histolojik değişimler için analiz edildi. Endotelyal ve vasküler fonksiyonlar immünhistokimyasal yöntem ile incelendi. Bulgular: D Vitamini veya Omega 3 kullanımının normal koşullar altında vena cava yapısında histomorfolojik değişikliklere yol açmadığı tespit edildi. Vitamin D ve Omega 3'ün birlikte kullanımı sonucunda sıçanların vena cava örneklerinde indüklenebilir nitrik oksit sentazın immünoekspresyonu azaltırken, vasküler endotelyal büyüme faktörünün arttığı tespit edildi. Sonuç: Sonuç olarak, D vitamini ve Omega 3'ün kombine takviyelerinin hücresel düzeyde damar yapısı üzerine zararlı bir etkisi olmadığına ancak faydalarının tespit edilmesi için daha kapsamlı çalışmalar yapılması kanaatine varıldı.

Anahtar Kelimeler

• Vitamin D
• Omega 3
• histopatoloji
• vena kava
• indüklenebilir nitrik oksit sentaz
• vasküler endotelyal büyüme faktörü

Kaynakça

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