Pages 24-29 The Comparision between the Modified Auramine Rhodamine Flourochrome Staining and Ziehl-Neelsen Staining Method from Sputum Specimens in the Presence with Radiometric Bactec Tb System

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Anahtar Kelimeler

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The Comparision between the Modified Auramine Rhodamine Flourochrome Staining and Ziehl-Neelsen Staining Method from Sputum Specimens in the Presence with Radiometric Bactec Tb System

Öz

Amaç: Akciğer tüberkülozunun teşhisi balgamın bakteriyolojik tetkiki ile mümkündür. Balgam yaymaların mikroskobik incelemesi etkilidir ve hastalığı doğrular. Fakat Balgam yaymaların mikroskobik incelemesinin duyarlılığı düşüktür. Çalışmamızda balgamdan Direkt yaymayla (DS) yapılan preparatların Modifiye– auramine flourokrom boyama (MR-AFS) yöntemiyle aside dirençli basillerin (AFB) boyanmasının kullanışlı olduğunu göstermeyi amaçladık. Hastalar ve Yöntem: Toplam 218 hastadan 10 pozitif kontrol ve 10 negatif kontrolden alınan AFB’in MR-AFS ve Ziehl-Neelsen Boyama ZNS yöntemleri ile BACTEC TB sistem altın standard olarak kabul edilerek incelendi ve kıyaslandılar. Bulgular: BACTEC TB sistemi ile 16 örnek, 13 örnek MR-AFS yöntemi ile ve 11 örnek ZNS AFS yöntemi ile pozitif olarak tespit edilmiştir. Pozitif olarak tespit edilen örneklerin oranları ZNS yönteminde MR-AFS yöntemine gore belirgin olarak yüksek olarak bulunmuştur (p < 0.01). BACTEC TB sistem altın standard olarak kabul edildiğinde MR-AFS’nin sensitivite ve spesivitesi sırasıyla %84 ve %100, ZNS’nin sensitivite ve spesivitesi sırasıyla %68.8 ve %100 olarak tespit edilmiştir. Sonuç: Klinik örneklerden AFB araştırılmasında MR-AFS yönteminin ZNS yöntemine göre daha hızlı pratik ve güvenli olduğu tespit edilmiştir. Ayrıca gelişmekte olan floresan mikroskobunun olduğu ülkelerde MR-AFS’nin kullanışlı bir yöntemdir

Anahtar Kelimeler

• Auramin, boyama, tuberküloz, balgam, kültür

Kaynakça

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29. Coresponding author: Teoman Zafer Apan
30. Kırıkkale Üniversitesi
31. Department of Microbiology and Clinical
32. Microbiology, Faculty of Medicine Kırıkkale, Türkiye Tel: [phone] Fax: [phone]
33. E-mail: [e-mail]