RESVERATROL, PALMİTAT İLE İNDÜKLENEN ENDOPLAZMİK RETİKULUM STRESİ İNHİBE EDER: ANTİ-APOPTOTİK ETKİ

Yıl 2016, , 68 - 74, 31.08.2016

Aslı F. Ceylan , Sinem Aslan Erdem

https://doi.org/10.24938/kutfd.252680

Öz

Giriş: Endoplazmik retikulum (ER) stresin obezite
patofizyolojisinde yeri bilinmesine karşın, obezite ile indüklenen kardiyovasküler
hastalıklarda (ER) stresin rolü tam olarak bilinmemektedir. Çalışmamız, doğal
bir polifenolik bileşik olan resveratrolün H9c2 hücre dizisinde serbest yağ
asidi olan palmitik asid (PA) ile indüklenen ER-stres üzerine olan etkilerini
incelemek amacıyla gerçekleştirilmiştir.

Gereç ve Yöntem: H9c2 hücre dizisi çeşitli konsantrasyon ve
zaman aralıklarında PA ile inkübe edilerek, ER-stres oluşumu sağlanmıştır.
Resveratrolün çeşitli konsantrasyonları kullanılarak, ER-stres yolağı ve bu
yolağın indükledği apoptotik yolak incelenmiştir.

Bulgular: PA doz ve süre bağımlı olarak
ER-stres yolağını aktive etmiştir. Bu yolak, apoptotik bir yolağın da
aktivasyonuna neden olmuştur. Resveratrol, PA ile indüklenen ER-stresi
azaltmakla birlikte, apoptozu da inhibe etmiştir. Bununla birlikte, PA hücresel
oksidatif stresin artmasına neden olurken, resveratrol antioksidan etkinik
sergilemiştir.

Sonuç: Sonuçlarımız, H9c2 hücrelerinde PA ile indüklenen ER-stresin
azaltılmasında ve apoptozun inhibe edilmesinde resveratrolün etkili olduğunu
göstermiştir. 

Anahtar Kelimeler

Apoptoz, endoplazmik retikulum stres, H9c2 hücre dizisi, palmitik asid, resveratrol

Resveratrol Inhibits Palmitic Acid Induced Endoplasmic Reticulum Stres: Anti-Apoptotic Effect

Öz

Introduction: Endoplasmic reticulum (ER) stress is involved in the pathophysiology of obesity but little is known about the role of ER-stress on obesity-induced cardiovascular diseases. Material and Methods: This study was designed to examine the effect of resveratrol, a natural polyphenolic compound, on palmitic acid-induced ER-stress in H9c2 cell lines. ER-stress was induced by various concentration of PA in different time manner in H9c2 cell lines. Effects of resveratrol on ER-stress and apoptosis pathways were examined in this model. Results: PA activated ER-stress pathway in a dose- and time-dependent manner. Indeed, apoptotic pathway was activated in the cells by PA. Resveratrol not only alleviated ER-stress but also inhibited apoptosis. On the other hand, PA increased oxidative stress in the cells which was alleviated by resveratrol. Conclusion: Our results suggest that, resveratrol play a role in alleviating ER-stress and inhibiting apoptosis in H9c2 cell lines.

Anahtar Kelimeler

Apoptosis, endoplasmic reticulum stress, H9c2 cell, palmitic acid, resveratrol

Kaynakça

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