Effects of Yucca Schidigera and Zeolite Supplementation to Diet on Blood Cytokines and Thyroid Hormones Concentrations in Sheep

Öz

This study is aimed to compare the effects of combined supplementation of Yucca schidigera (YS) and clinoptilolite as a zeolite source to diet on the blood IL-1β, IL-6 and TNF-α and thyroid hormones concentrations in sheep. A total of 24 sheep were divided four groups as the Control, YS group, Zeolite and YS + Zeolite group and each group was contained 6 animals. The basal diet was given to animals in control group, while the animals in the Zeolite, YS and YS + Zeolite groups were fed with the experimental diet contained the basal diet plus 3% clinoptilolite, 1500 ppm YS and 1500 ppm YS + 3% clinoptilolite, respectively. The blood samples were taken from the animals in the end of experimental period lasted 30 days. The plasma IL-1β, IL-6, TNF-α, T3 and T4 concentrations were determined by using the commercial ELISA. The supplementation of YS decreased the plasma IL-1β concentration and the combined supplementation of Zeolite and YS lowered the plasma TNF-α concentration. The plasma IL-6, T3 and T4 concentrations were not affected by the treatments. As a result, it was concluded that the combined supplementation of zeolite and YS, which are two natural feed additives, instead of adding them separately to the feed, may affect the proinflammatory cytokines production in sheep blood. 

Anahtar Kelimeler

• zeolite
• cytokines
• Thyroid hormones
• sheep
• saponins

Koyunlarda Yeme Yucca Schidigera ve Zeolit Katılmasının Kan Sitokin ve Tiroid Hormon Düzeylerine Etkileri

Öz

Bu çalışmanın amacı, koyunlarda yeme steroidal saponin içeren Yucca schidigera (YS) ve zeolit kaynağı olarak klinoptilolitin birlikte ilavesinin kan IL-1β, IL-6, TNF-α ve tiroid hormon düzeylerine etkilerini karşılaştırmaktır. Çalışmada, toplam 24 koyun kullanıldı ve koyunlar her biri 6 hayvan içeren Kontrol, YS, Zeolit ve YS + Zeolit olmak üzere dört gruba ayrıldı.Kontrol grubundaki hayvanlar temel yemle beslenirlerken, YS gruptaki hayvanlar yeme 1500 ppm düzeyinde YS ilave edilmiş yemle, Zeolit gruptaki hayvanlar %3 düzeyinde klinoptilolit ilave edilmiş yemle ve YS+Zeolit gruptakiler ise 1500 ppm YS ile %3 klinoptilolit ilave edilmiş yemle beslendiler. Toplam 30 gün süren deneme döneminin sonunda, hayvanlardan kan örnekleri alındı. IL-1β, IL-6, TNF-α, T3 ve T4'ün plazma düzeyleri, ticari kitler kullanılarak ELİSA cihazında belirlendi. Yeme YS ilavesi plazma IL-1β düzeiyini azalttırken, YS+Zeolitin birlikte yeme ilavesi ise plazma TNF-α düzeyini düşürdü. Buna karşın, çalışmada, uygulamaların IL-6, T3 ve T4 düzeylerine etkisinin olmadığı tespit edildi. Sonuç olarak, iki doğal yem katkı maddesi olan zeolit ve YS’nın yeme ayrı ayrı katılması yerine birlikte ilavesinin koyun kanında proinflamatuar sitokinlerin üretimini etkileyebileceği kanaatine varıldı.

Anahtar Kelimeler

• zeolit
• sitokinler
• tiroid hormonları
• koyun
• saponinler

Kaynakça

1. Bartko, P., Vrzgula, L., Prosbova, M., Blazovsky, J. 1983.The effect of feeding zeolite (clinoptilolite) on the health status of sheep. Vet.Med. (Praha). 1983; 28: 481-492.
2. Bodas, R., Prieto, N., Garcia-Gonzalez, R., Andres, S., Giraldez, F.J., Lopez, S. Manipulation of rumen fermentation and methane production with plant secondary metabolites. Anim Feed Sci Technol., 2012; 176:78-93.
3. Cammack, KM., Austin, K J., Lamberson, WR., Conant, GC., Cunningham, HC. RUMINNAT NUTRITION SYMPOSIUM: Tiny but mighty: the role of the rumen microbes in livestock production. J. Anim. Sci., 2018; 96:752–770. doi:10.1093/jas/skx053
4. Chattopadhyay, M.K. Use of antibiotics as feed additives: a burning question. Front. Microbiol., 2014; doi.org/10.3389/fmicb.2014.00334
5. Chen, F., Lv Y., Zhu, P., Cui, C., Wu, C., Chen, J., Zhang, S., Guan, W. Dietary Yucca schidigera Extract supplementation during late gestating and lactating sows improves animal performance, nutrient digestibility, and manure ammonia emission. Frontiers Vet.Sci., 2021; 8: 676324. DOI:10.3389/fvets.2021.676324
6. Çolpan, İ. Kanatlı beslenmesinde zeolitin etkileri. Vet.Tavuk.Dern.Derg.Mektup, Ankara, 2013; 11:12-22. de Sousa, O.A., Cooke, R.F., Brandão, A.P., Schubach, M.K., Schumaher, T.F., Bohnert, D.W., Marques, R.S. Productive and physiological responses of feeder cattle supplemented with Yucca schidigera extract during feedlot receiving. J Anim. Sci., 2019; 97:208–219.
7. Durmuş, İ., Evcimen, M., Salim, M.N., Küçükkurt, İ., İnce, S., Eryavuz, A. Koyunlarda yemlemeden sonra kan oksidan-antioksidan denge ile bazı biyokimyasal parametrelerdeki değişimlerin belirlenmesi. Kocatepe Vet.J., 2017; 10:1-6.
8. EFSA Panel on Additives and Products or Substances used in Animal Feed. Scientific Opinion on the safety and efficacy of clinoptilolite of sedimentary origin for all animal species. EFSA J., 2013; 11: 1–14.

9. El-Nile, A., Elazab, M., El-Zaiat, H., El-Azrak, K., Elkomy, A., Sallam, S., Soltan, Y. In vitro and in vivo assessment of dietary supplementation of both natural or nano-zeolite in goat diets: Effects on ruminal fermentation and nutrients digestibility. Animals, 2021; 11: 2215. https://doi.org/10.3390/ani11082215
10. Elsabahy, M., Wooley, K.L. Cytokines as biomarkers of nanoparticle immunotoxicity, Chem. Soc. Rev., 2013; 42: 5552–5576.
11. Eryavuz A., Küçükkurt İ., İnce S., Fidan AF., Avcı G., Bülbül T. Kuzularda Rasyona Yucca Schidigera Tozu Katılması ve Günlük Dozunun Rumen Fermentasyonu ile Verime Etkilerinin Araştırılması. Kocatepe V.J., 2015; 8: 1-10.
12. Huszenicza, GY., Kulcsar, M., Rudas, P. Clinical endocrinology of thyroid gland function in ruminants. Vet. Med. (Czech), 2002; 47: 199–210.
13. Kandır, S., Keskin, E. Serum IL-1β, IL-6, IL-10 and TNF-α Levels in Thyroidectomized Rats. Kafkas Univ Vet Fak Derg., 2016; 22: 297-300.
14. Klasing, K. C. Nutrition and the immune system. Br. Poult.Sci., 2007; 48:525–537.
15. Kucukkurt I, Akkol EK, Karabag F, Ince S, S€untar I, Eryavuz A, Sozbilir NB. Determination of the regulatory properties of Yucca schidigera extracts on the biochemical parameters and plasma hormone levels associated with obesity. Rev Bras Farmacogn., 2016; 26: 246–250.
16. Kutlu HR, Serbester U. Ruminant beslemede son gelişmeler. Türk Tarım – Gıda Bil.Tek, Derg., 2014; 2(1): 18-37.
17. Lai X, Pei QS, Song X, Zhou X, Yin ZQ, Jia RY, Zou YF, Li LX, Yue GZ, Liang XX, et al. The enhancement of immune function and activation of NF-jB by resveratrol-treatment in immunosuppressive mice. Int Immunopharmacol., 2016; 33:42–47. Marchiori, R.C., Pereira, L.A., Naujorks, A.A., Rovaris, D.L., Meinerz, D.F., Duarte, M.M., Rocha, J.B. Improvement of blood inflammatory marker levels in patients with hypothyroidism under levothyroxine treatment. BMC Endocr. Disord., 2015; 15:32 DOI 10.1186/s12902-015-0032-3.
18. Oelschlager, M.L., Rasheed, M.S.A., Smith, B.N., Rincker, M.J., Dilger, R.N. Effects of Yucca schidigera-derived saponin supplementation during a mixed Eimeria challenge in broilers. Poultry Science, 2019; 98: 3212–3222.
19. Piacente S, Pizza C, Oleszek W. Saponins and phenolics of Yucca schidigera Roezl: chemistry and bioactivity. Phytochem Rev., 2005; 4:177–190.
20. Shaaban, M.M., Kholif, E.A., El Tawab, A.M., Radwan, M. Thyme and celery as potential alternatives to ionophores use in livestock production: their effects on feed utilization, growth performance and meat quality of Barki lambs.Small Rum.Res., 2021; 200: 106400. DOI:10.1016/j.smallrumres.2021.106400
21. Su JL, Shi BL, Zhang PF, Sun DS, Li TY, Yan SM. Effects of yucca extract on feed efficiency, immune and antioxidative functions in broilers. Braz Arch Biol Technol., 2016; 59:e16150035.
22. Suwa T, Hogg JC, English D, van Eeden SF. Interleukin-6 induces demargination of intravascular neutrophils and shortens their transit in marrow. Am. J. Physiol. Heart Circ. Physiol., 2000; 279: H2954–60.
23. Tayde, P., Bhagwat, N., Sharma, P., Sharma, B., Dalwadi, P., Sonawane, A., Subramanyam, A., Chadha, M., Varthakavi, P. Hypothyroidism and depression: are cytokines the link? Indian J. Endocrinol. Metab., 2017; 21:886-892.
24. Tirtaatmadja, N.; Mortimer, G.; Ng, E.-P.; Ahmad, H.; Mintova, S.; Serpooshan, V.; Minchin, R.; Mahmoudi, M. Nanoparticles-induced inflammatory cytokines in human plasma concentration manner: An ignored factor at the nanobio-interface. J. Iran. Chem. Soc. 2015; 12: 317-323.
25. Wu Y., Wu Q., Zhou Y., Ahmad H., Wang T. Effects of Clinoptilolite on Growth Performance and Antioxidant Status in Broilers. Biol. Trace Elem. Res., 2013; 155: 228-235.
26. Zhang CY, Tian YD, Yan FB, Kang XT, Han RL, Sun GR, Zhang HR. Modulation of growth and immunity by dietary supplementation with resveratrol in young chickens receiving conventional vaccinations. Am. J. Vet. Res., 2014; 75:752–759.