Holştayn Irkı Düvelerde Pubertas Öncesinde Rasyona Rumen Korumalı Lizin Ve Metiyonin İlavesinin Bazı Kan Metabolizma Parametreleri Üzerine Etkileri

Öz

Bu çalışmada Holştayn ırkı düvelerde yaşamın 9. ayından başlanarak gebeliğin belirlendiği zamana kadar rasyona rumen korumalı lizin ve metiyonin ilavesinin bazı kan metabolizma parametreleri üzerine etkileri incelenmiştir. Bu amaçla 40 adet sağlıklı ve 9 aylık yaşı doldurmuş (9-10 ay arası) Holştayn ırkı düveler rastgele örnekleme metodu ile K (Kontrol) ve U (Uygulama) olmak üzere 2 gruba ayrılmıştır. Kontrol grubundaki düveler NRC (2001)’e göre hazırlanmış standart bir rasyonla, uygulama grubundaki düveler ise rumen korumalı amino asitler kullanılarak (Lysigem ve Methipearl, Kemin Ind., Belgium) lizin (MP’nin %7.1’i) ve metiyonin (MP’in %2.4’ü) düzeyleri artırılmış bir rasyonla ad-libitum olarak beslenmiştir. Tüm düvelerden çalışma başlangıcında, tohumlama zamanında ve gebeliğin tespit edildiği gün vena coccygea yolu ile kan numunesi alınmıştır. Alınan kan numuneleri ilgili kitler kullanılarak glukoz (GLU), betahidroksibütirat (BHBA), esterleşmemiş yağ asitleri (NEFA), total kolesterol (TK), trigliserit (TG), total protein (TP) ve kan üre nitrojeni (BUN) analizleri yapılmıştır. Çalışmada kan metabolizma parametrelerinden GLU, NEFA ve BHBA düzeylerinin prepubertas, tohumlama ve gebelik tespit edilen dönemlerde istatiksel olarak anlamlı şekilde değiştiği (p<0.0001), TK, TP ve BUN düzeylerinin ise anlamlı şekilde değişmediği (p>0.5) tespit edilmiştir. Ayrıca rumen korumalı lizin ve metiyonin kullanılarak rasyonun metabolik lizin ve metiyonin düzeyinin artırılmasının tohumlama zamanında kanda TK seviyesini artırdığı (p<0.005), TP seviyesini ise düşürdüğü belirlenmiştir (p<0.05).

Anahtar Kelimeler

• Holştayn
• Düve
• Serum Metabolites
• Prepuertal

Effects of Rumen Protected Lysine and Methionine Supplementation on Some Blood Metabolic Parameters in Prepubertal Holstein Heifers

Öz

The aim of this study was to investigate the effects of supplemental rumen protected lysine and methionine on some blood metabolic parameters in prepubertal Holstein heifers. Forty, healthy, 9-month-old heifers were divided into two groups as control (C) and treatment (T). C heifers were fed a standard diet which has been prepared according to NRC (2001), whereas T heifers were fed a lysine (7.1% of MP) and methionine (2.4% of MP) enriched (Lysigem and Methipearl, Kemin Ind., Belgium) diet. Blood samples were taken from all heifers at the beginning of the study, on the day of artificial insemination and on the day of pregnancy detection. All blood samples were analyzed for glucose (GLU), beta-hydroxybutyric acid (BHBA), non-esterified fatty acids (NEFA), total cholesterol (TCHOL), triglycerides, total protein (TP) and blood urea nitrogen (BUN). It was determined that the levels of GLU, NEFA and BHBA, which are blood metabolism parameters, changed statistically (p<0.001) in the periods when prepubertal, insemination and pregnancy were detected, while the levels of TKOL, TRIG and BUN did not change significantly. Moreover, increasing lysine and methionine content of the prepubertal diet caused an increase in serum TCHOL concentration (p<0.005) whereas decreased serum TP concentration (p<0.05)

Anahtar Kelimeler

• Holstein
• Heifer
• Serum Metabolites
• Prepubertal

Kaynakça

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