Determination of In vitro Antioxidant Activities and Macro and Micro Elements Level in Different Extracts of Cynara Scolymus L. leaf

Yıl 2022, , 412 - 422, 31.12.2022

Sümeyra Albayrak , Barış Denk , Büşra Karpuz , Esra Küpeli Akkol , Gülcan Avcı

https://doi.org/10.30607/kvj.1157121

Öz

Globe Artichoke (Cynara scolymus L.) belonging to the family of Astericaea, has antioxidant, hepatoprotective and hypoglycemic activities, its leaves have traditionally been used for diuretic and choleretic purposes. Therefore the main goal of this study is to determine the total phenolic content of artichoke leaf and its extracts with methanol, ethyl acetate and n- hexane, some in vitro antioxidant activities, selected macro (Na, K, Ca, Mg, P) and micro (Zn, Cu, Mn, Cr, Se, I) elements’ levels. Accordingly the total phenolic content values for the methanol, ethyl acetate and N-hexane extracts found 5,375 mg, 0,917 mg, 0,167 mg Gallic acid (GAE)/g respectively. Methanol extract showed highest DPPH free radical scavenging activity (87,73%), ethyl acetate extract possessed the highest Superoxide radical scavenging activity (SRSA) (49,02 %) whereas N-hexane extract contained high level metal chelating ability ( 289,32µM Fe). In terms of macro and micro elements (except I and Cr levels), the highest concentrations are recorded in its leaves, which are considered as a natural mineral source. Accordingly, it is evaluated that artichoke leaves provide a potential natural sources of K and Zn, while methanol and N-hexane extracts are good sources of P and Zn.

Anahtar Kelimeler

Antioxidant activity, artichoke, Cynara scolymus L, leafextracts, minerals

Destekleyen Kurum

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Proje Numarası

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Teşekkür

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Cynara scolymus L. Yaprağının Farklı Ekstraktlarda In vitro Aktioksidan Aktivitelerinin ve Makro ve Mikro Element Seviyelerinin Belirlenmesi

Öz

Papatyagiller familyasına ait olan Küre enginarın (Cynara scolymus L.) antioksidan, hepatoprotektif ve hipoglisemik etkilere sahiptir, yaprakları geleneksel olarak idrar söktürücü ve koleretik amaçlarla kullanılmaktadır. Bu nedenle bu çalışmanın temel amacı enginar yaprağının ve yaprağın metanollü, etil asetatlı ve n-hekzanlı ekstraktlarının toplam fenolik içeriğini bazı in vitro antioksidan aktiviteleri, seçilmiş makro (Na, K, Ca, Mg, P) ve mikro (Zn, Cu, Mn, Cr, Se, I) element düzeylerini, belirlemektir. Toplam fenolik içerik değerlerine göre metanol, etil asetatlı ve n-hekzan ekstraktları sırasıyla 5,375 mg, 0,917 mg, 0,167 mg Gallik asit (GAE)/g bulunmuştur. Metanol ekstraktı en yüksek DPPH serbest radikal süpürme aktivitesi (%87,73) gösterirken, etil asetat ekstraktı en yüksek süperoksit radikal süpürme aktivitesine (%49,02) sahip iken n-hekzan ekstraktı yüksek seviye metal şelatlama kapasitesi (289,32 µM Fe) içermektedir. Makro ve mikro elementler açısından (I ve Cr seviyeleri hariç) en yüksek konsantrasyonlar doğal mineral kaynağı olarak kabul edilen yapraklarında kaydedilmiştir. Buna göre enginar yapraklarının potansiyel bir doğal K ve Zn kaynağı sağladığı, metanol ve n-hekzan ekstraktlarının ise iyi P ve Zn kaynağı olduğu değerlendirilmektedir.

Anahtar Kelimeler

Antioksidan aktivite, Cynara scolymus L, enginar, mineraller, yaprak ekstraktları

Proje Numarası

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Kaynakça

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